新生小鼠缺氧缺血脑组织基质细胞衍生因子1α的表达及其作用

doi:10.6040/j.issn.1671-7554.0.2015.097

Abstract

Abstract: Objective To explore the expression of stromal cell derived factor-1α(SDF-1α) in the brain of mice after hypoxic ischemic brain damage (HIBD), and to discuss the effects of SDF-1α on the expression of ki67 and the improvement of neurological behavior. Methods Ninety KM newborn mice were devided into blank control group, HIBD model group and SDF-1α group, with 30 mice in each group. HIBD model group and SDF-1α group were intraperitoneal injected with 0.9% NaCl and SDF-1α(2.0 μg/d in each group) respectively at 30 min after HIBD modeling. Three subgroups were intraperitoneal injected with SDF-1α for 1, 3, 7 d, while blank control group were injected with equivalent 0.9% NaCl. SDF-1α expression at different time points were observed by immunofluorescence and Real-time PCR. ki67 expression was observed by immunofluorescence and Western blotting. Neurobehavioral improvement were recorded by elevated plus maze. Results SDF-1α expression in HIBD model group increased and reached a peak on the third day, and the difference between this group and blank control group was statistically significant (P<0.01). ki67 expression significantly increased in SDF-1α group, compared with that in HIBD model group (P≤0.01). Theneurological behavior of mice in SDF-1α group also was improved, compared with HIBD model group(P<0.05). Conclusion SDF-1α expression is upregulated in HIBD model group, and reaches a peak on the third day. Exogenous SDF-1α induces the expression of ki67, and improves the learning ability of mice with HIBD.

Key words: Brain hypoxic-ischemic, ki67, Neurobehavioral, Brain injuries, Stromal cell derived factor-1α

CLC Number:

R725

LI Yuanyuan, ZHANG Nan, XU Mi, QIN Mingming, DOU Dongdong, ZHU Weiwei. Expression of stromal cell derived factor-1α and its effects in hypoxic-ischemic brain tissue of neonatal mice[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(10): 26-31.

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Expression of stromal cell derived factor-1α and its effects in hypoxic-ischemic brain tissue of neonatal mice

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