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Role of tumor-associated macrophages in epithelial-mesenchymal transition of human hepatocellular carcinoma cells

WANG Hao1, LI Xia2, WANG Chao3, LI Guosheng1, GUO Chun1, ZHU Faliang1, ZHANG Lining1,  SHI Yongyu1   

  1. 1. Institute of Immunology, School of Medicine, Shandong University, Jinan 250012， Shandong， China;
    2. Department of Oncology, Jinan First People’s Hospital, Jinan 250011， Shandong， China;
    3. Department of General Surgery, Jinan First People’s Hospital, Jinan 250011, Shandong， China
  • Received:2013-09-11 Online:2014-04-10 Published:2014-04-10

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Abstract: Objective  To explore the effect and mechanism of tumor-associated macrophages on  human hepatocellular carcinoma (HCC) cells. Methods  The HCC cells were cocultured with macrophages from PMA-treated THP-1 cells and cell migration was detected by transwell migration test and wound-healing assay. The expressions of E-cadherin and N-cadherin were measured by RT-PCR. Results  The ability of cell migration was significantly increased after being cocultured with mocrophages from PMA-treated THP-1 cells. A transition from epithelial morphology to mesenchymal morphology was observed. The expression of E-cadherin decreased and the expression of N-cadherin increased. Conclusion  Our findings suggest that tumor-associated macrophages may promote the cell migration through epithelial-mesenchymal transition.

Key words: Coculture, Epithelial-mesenchymal transition, Hepatocellular carcinoma, Tumor-associated macrophages

CLC Number: 

  • R392.11
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