JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effects of enhanced expression of RbAp48 mediated by recombinant adenovirus on proliferation and growth of cervical cancer cells

ZHANG Wen1, FENG Tingting1, ZHENG Lin1, WANG Hong1, LU Yi2, QI Mei1, YU Xiuping1, TANG Wei1, ZHAO Weiming1   

  1. 1. Department of Medical Microbiology;  2. Department of Biochemistry and Molecular Biology,
     School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2013-12-20 Online:2014-04-10 Published:2014-04-10

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Abstract: Objective  To construct the recombinant adenovirus carrying RbAp48 gene, and to study its effect on the behaviors of human cervical cancer cell line SiHa. Methods  The shuttle plasmid pDC316-RbAp48 was constructed and co-transfected into the HEK293 cells with genomic plasmids pBHGlox ΔE1, 3Cre to produce the replication-deficient recombinant adenovirus Ad5-RbAp48. Then the SiHa cells were infected with Ad5-RbAp48, and RbAp48 expression was examined by immunoblotting. WST-8, cell number counting, and soft agar assay were carried out to examine the effects of Ad5-RbAp48 infection on cell proliferation and anchorage-independent growth of SiHa cells. Results  Ad5-RbAp48 was successfully constructed. Ad5-RbAp48 infection significantly enhanced the expression of RbAp48 in SiHa cells, and effectively inhibited cell proliferation and anchorage-independent growth. Conclusion  Recombinant adenovirus carrying RbAp48 gene can mediate RbAp48 expression and inhibit behaviors of cervical cancer cells.

Key words: RbAp48, Cervical cancer, Adenovirus, Gene expression

CLC Number: 

  • R37
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