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Effect of PDCD4 siRNA on the lipid accumulation in macrophages#br#

DONG Zhaojing, SHANG Qianwen, GUO Chun, ZHANG Lining, WANG Qun   

  1. Department of Immunology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2013-06-21 Online:2014-04-10 Published:2014-04-10

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Abstract: Objective  To investigate the effect of PDCD4 on lipid accumulation in macrophages. Methods  The cells were divided into PDCD4 siRNA group and negative control siRNA group. Gene silencing was performed in murine macrophages using PDCD4 siRNA and negative control siRNA; the mRNA and protein levels of PDCD4 were detected by RT-PCR and Western blotting; after treatment with ox-LDL, the lipid contents in macrophages were detected by Oil red O staining; the expressions of cholesterol efflux-related genes liver X receptor α (LXR-α), ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) were detected by Real-Time PCR. Results  PDCD4 siRNA effectively reduced the expression of PDCD4 on both mRNA and protein levels in macrophages; the lipid contents decreased significantly in PDCD4 siRNA group compared with those in negative control siRNA group (P＜0.001), and the expressions of LXR-α (P＜0.05), ABCA1 (P＜0.01) and ABCG1 (P＜0.001) were markedly  up-regulated. Conclusion  Silencing of PDCD4 gene can reduce the lipid accumulation in macrophages through promoting cholesterol efflux.

Key words: Macrophages, Programmed cell death 4, RNA interference, Lipid

CLC Number: 

  • R392.11
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