Abstract: Objective  To study the effect of heavy drinking on angiogenesis in ischemic rats and the effect of Rosuvastatin on angiogenesis after heavy drinking. Methods  A total of 24 male SD rats were randomly divided into 3 groups: control group, heavy-drinking group［50% ethanol, 5g/(kg·d)］ and Rosuvastatin pretreated group［Rosuvastatin (20mg/(kg·d)+50% ethanol, 5g/(kg·d)］. The three groups were given equal volume of ethanol, rosuvastatin and vehicle by gavage respectively for 4 weeks and then myocardial infarction models were constructed. The rats were killed on the 3rd day after myocardial infarction. The microvascular density (MVD) was observed by immunohistochemical SP method. The vascular endothelial growth factor (VEGF) and endostatin expressions in the ischemia myocardium tissue were tested with ELISA. Results  Compared to the control group, the MVD was significantly reduced (P<0.01) in the heavy-drinking group, the level of endostatin was significantly increased (P<0.01), and there was no difference in the expression of VEGF. MVD in the Rosuvastatin pretreated group was significantly increased compared to that in the heavy-drinking group (P<0.01), but the VEGF and endostatin expressions were not significantly different between the two groups(P＞0.05). Conclusion  Heavy drinking could inhabit angiogenesis by increasing the expression of endostatin after myocardial infarction. Rosuvastatin could reverse the damages of heavy drinking on angiogenesis.

Key words: Rosuvastatin, Myocardial infarction, Angiogenesis, Heavy drinking