Abstract:

bjective  To investigate the effects of Rosuvastatin on the expressions of SIRT1 and p-FOXO3a in the ApoE-/- mice cardiac myocytes.  Methods  A total of 10 C57BL/6J mice were randomly selected as the control group, which were given ordinary diet. Another 20 ApoE-/- mice were randomly divided into the model group (n=10) and treatment group (n=10), which were fed with high lipid diet. The treatment group underwent Rosuvastatin suspension gavage, whereas the model group received the same volume of normal saline for 16 weeks. Serum lipid was detected with full-automatic biochemical instrument. The expressions of SIRT1 and p-FOXO3a were evaluated with Western  blotting. The expressions of SIRT1, SOD and MDA were detetced with immunohistochemical method.  Results  TC, TG, LDL-C and MDA significantly increased in the model group than in the control group, but the levels of HDL-C and SOD were lower in the model group (P<0.05), and the myocytes were hypertrophic. Meanwhile, the expressions of SIRT1 and p-FOXO3a protein in cardiac myocytes of the model group significantly decreased (P<0.05).Compared with the model group, the levels of TC, TG, LDL-C and SOD significantly decreased whereas HDL-C and MDA increased in the treatment group (P<0.05). The expressions of SIRT1 and p-FOXO3a protein in cardiac myocytes markedly increased and myocardial cells arranged orderly.  Conclusion  ApoE-/-  mice fed with high-fat diet are prone to oxidative stress injury in cardiac myocytes, while Rosuvastatin can up-regulate the expressions of SIRT1 and p-FOXO3a, protecting the myocardium from such injury.

Key words: Oxidative Stress, Rosuvastatin, SIRT1, ApoE-/-, FOXO