JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 26-29.doi: 10.6040/j.issn.1671-7554.0.2014.236

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Impact of aging on the nigro-strital oxidative stress in a mice model of Parkinson's disease

ZHANG Zhongxia, MA Xiaowei, WANG Yanyong, LI Xiaoli, WANG Mingwei   

  1. Department of Neurology, the First Hospital of Hebei Medical University, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei, China
  • Received:2014-03-21 Revised:2014-06-23 Online:2014-09-10 Published:2014-09-10
  • Contact: 王铭维。E-mail:wmw@hebmu.edu.cn E-mail:wmw@hebmu.edu.cn

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Abstract: Objective To observe the age-related changes of nigro-strital oxidative stress in a mice model of Parkinson's disease(PD) at different ages and detect the differential expression of oxidative stress related genes in aged PD mice by using PCR Array. Methods Forty-two healthy female senescence-accelerated mouse prone 8 (SAMP8) mice aged 3, 6 and 10-month were averagely and randomly divided into 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) group and control group, which were subcutaneously injected with MPTP or the same volum of 0.9% NaCl, respectively. After the first injection for 72 hours, behavioral changes in mice were examined by the open field test. The levels of dopamine (DA) in nigro-striatal system was measured by a high performance liquid chromatography with electochemical detector (HPLC-ECD). The activities of superoxide dismutase 1(SOD1) and the content of malondialdehyde(MDA) were detected by the spectrophotometer. The injuries of nigraldopamine system and striatal oxidative stress related indexes were compared among mice at different ages. The expression of striatal oxidative stress related gene expression in 10-month mice was detected by PCR Array. Results Compared with control group, the levels of DA andSOD 1, the performance in the open field test all decreased in MPTP group at three ages, while the content of MDA in tissue remarkably increased (P<0.01). Moreover, the above changes in 10-month mice were more obvious than 3- and 6-month mice (P<0.05). Compared with control group, the PCR Array results of MPTP group showed that COX-2 was up-regulated, while glutathione peroxidase 3, 6 and 8, lactoperoxidase, nucleoredoxin, myoglobin, neuroglobin, peroxiredoxin 1, eosinophil peroxidase were all down-regulated (fold change>2). Conclusion Aging plays an important role in nigro-striatal system injury of PD model. Up- or down- regulation of oxidative stress related genes may participate in the early phase of PD.

Key words: Oxidative stress, Gene differential expression, Parkinson’s disease, Aging, Senescence-accelerated mouse prone 8

CLC Number: 

  • R741
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