JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 20-25.doi: 10.6040/j.issn.1671-7554.0.2014.203

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Juices of cherry and strawberry confer protection of human bronchial epithelial cells against arsenic-induced toxicity via activation of Nrf2 signaling pathway

KONG Degang1, WANG Lili2, ZHOU Honglei1, REN Dongmei3, LOU Hongxiang3, SHEN Tao3   

  1. 1. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;
    2. Division 1, Department of Paediatrics, Peopel's Hospital of Rizhao, Rizhao 276800, Shandong, China;
    3. Key Lab of Chemical Biology Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-04-08 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 沈涛。E-mail:shentao@sdu.edu.cn E-mail:shentao@sdu.edu.cn

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Abstract: Objective To investigate the effects of cherry and strawberry juices on NF-E2-related factor 2(Nrf2) signaling pathway, and to evaluate their protection effects on arsenic-induced toxicity in human bronchial epithelial (HBE) cells. Methods Human breast cancer 231 cells were randomly divided into the control group, sulforaphen treated group, and juice treated groups (juice dilution ratios were 1:10,1:20,1:40 and 1:80, respectively), and then antioxidant response element(ARE) luciferase reporter gene assay and Western blotting analysis were adopted for screening and identification of juice on Nrf2 signaling pathway; HBE cells were randomly divided into the control group, sul-foraphen treated group, and juice treated groups (juice dilution ratios were 1:10,1:20, and 1:40, respectively), and then the QuantiChrom glutathione (GSH) assay kit were used to detect the intracellular GSH levels; MTT assay and cell transfection were carried out to evaluate their protection effects against arsenic-induced toxicity in HBE cells. Results Juices of cherry and strawberry increased the luciferase activity in a dose-dependent manner, enhanced the protein expression of Nrf2 and its downstream genes NADPH:quinone oxidoreduefase Ⅰ(NQO1) and gamma glutamyl cysteine synthetase (γGCS) in MDA-MB-231 cells, and up-regulated the intracellular GSH levels in HBE cells. Co-treatment with juice and arsenic evidently improved HBE cell survival in response to arsenic treatment alone, while the juices no longer protected the HBE cells whose Nrf2 expression was koncked down by Nrf2-siRNA. Conclusion Juices of cherry and strawberry confer the protection effects on HBE cells against arsenic-induced toxicity in a Nrf2-dependent manner.

Key words: Nrf2 signaling pathway, Cherry, Strawberry, Arsenic, Human bronchial epithelial cells

CLC Number: 

  • R966
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