JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (10): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.1000

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Role of Caveolin-1 in postischemic angiogenesis in a rat model of ischemic stroke

ZHENG Juan1, LI Zheng2, ZHANG Wenjing1, YUAN Linran1, FAN Shubo1, LIU Yugang1, YUAN Zhongrui1   

  1. 1. Department of Pathophysiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. The Second Hospital of Kunming Medical University, Kunming 650101, Yunnan, China
  • Received:2014-12-26 Online:2015-10-10 Published:2015-10-10

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Abstract: Objective To investigate the expression and involvement of Caveolin-1(Cav-1) in postischemic angiogenesis using a rat model of ischemic stroke. Methods Normal male Wistar rats were randomly divided into four groups: Sham group(n=15), cerebral ischemia group (MCAO group, n=55), lentivirus-expressing Cav-1 siRNA treated group (siRNA group, n=20) and lentivirus-expressing nonsense siRNA treated group (misRNA group, n=20). Q-PCR and Western blotting analysis were used to detect the expression of Cav-1. FITC-dextran was used to detect the vessel volume, and Laser Doppler was used to measure the local cerebral blood flow in ischemic penumbral. Results The expressions of Cav-1 from hour 4 to day 3 post-MCAO significantly decreased in MCAO group (all P<0.05), compared with sham group, and the minimum level appeared on day 3 post-MCAO(P<0.01). Interestingly, the expression of Cav-1 on day 7 post-MCAO significantly restored (P<0.05), and reached the normal level on day 14 post-MCAO(P>0.05). As expected, in Cav-1 siRNA group, the expression of Cav-1 significantly decreased compared with MCAO or misRNA group (all P<0.05). Meanwhile, there were significantly weakened vessel volume and local cerebral blood flow in ischemic penumbral (P<0.05). Conclusion Cav-1 might be involved in postischemic angiogenesis. Further investigation of the regulation molecular machenism of Cav-1 in postischemic angiogenesis may be helpful for therapeutic angiogenesis for ischemic stroke.

Key words: Rats, Angiogenesis, Ischemic stroke, Caveolin-1

CLC Number: 

  • R614.2
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