JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.271

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RNAi-mediated silencing of CD147 inhibits the proliferation and invasion of leukemic cells SHI-1

TU Yan1,2, LI Zhenjiang1, TANG Aiping1, FEI Yan1, LI Huihui1, LI Jian1, HE Wenfeng1   

  1. 1. Department of Hematology, Second Hospital Affiliated to Nanchang University, Key Laboratory of Hematology in Jiangxi Province, Nanchang 330006, Jiangxi, China;
    2. Department of Hematology, Jinhua Hospital of Zhejiang University, Jinhua 321000, Zhejiang, China
  • Received:2014-04-14 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 李振江。E-mail:lzjdgh@163.com E-mail:lzjdgh@163.com

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Abstract: Objective To construct lentivirus vector for RNA interference (RNAi) targeting extracellular matrix metalloproteinase inducer (CD147) and to investigate the role of CD147 gene on the proliferation and infiltration of a human monocytic leukemic cell line SHI-1. Methods The complementary shRNA targeting CD147 was synthesized, and connected with pGCSIL-GFP vector. The 293T cells were transfected with recombined viral vector, packing plasmid pHelper 1.0 and pHelper 2.0 using Lipfectamine 2000. Then the recombinant lentiviruses were collected and infected the SHI-1 cells. The expressions of CD147, MMP-2 and MMP-9 in SHI-1 cells were detected by real time PCR. The protein of CD147 was detected by Western blotting. The capability of proliferation and infiltration of SHI-1 cell were examined by MTT and trans-matrigel invasion assay co-cultured with leukemia BMSCs in vitro. Results The lentivirus containing the CD147 shRNA was constructed successfully. The titer of recombined virus was 1×109 TU/mL. The mRNA and protein of CD147 in SHI-1/CD147i cells decreased by 86.7% and 91% respectively after the SHI-1 cells were infected by the lentivirus containing the CD147 siRNA. The proliferation capability of SHI-1/CD147i cells significantly decreased compared with that of SHI-1 and SHI-1/NC cells. The mRNA expressions of MMP-2 and MMP-9 in SHI-1/CD147i cells were significantly lower than that in SHI-1/NC and SHI-1 cells. The SHI-1/CD147i cells demonstrated significantly lower invasion rate than SHI-1 cells and SHI-1/NC cells when co-cultured with BMSCs. Conclusion CD147 plays important roles in the leukemia cell proliferation and infiltration. CD147 should be a potential target for the treatment of acute leukemia.

Key words: matrix metalloproteinase, Extracellular matrix metalloproteinase inducer, RNA interference, Cell invasion, Acute monocytic leukemia cell line, Cell proliferation

CLC Number: 

  • R733.7
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