JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (4): 50-54.doi: 10.6040/j.issn.1671-7554.0.2016.728

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Preparation and characteristics of novel nanoscaled ultrasound contrast agents

DUAN Sujuan, SHI Dandan, SHANG Mengmeng, GUO Lu, SUN Xiao, MENG Dong, LI Jie   

  1. Department of Ultrasound, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-06-21 Online:2017-04-10 Published:2017-04-10

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Abstract: Objective To prepare lipid nanoscaled ultrasound contrast agents(UCAs), and to explore their physiochemical properties. Methods Orthogonal test was used to screen out the optimal condition for preparation aiming at three mainly factors. Amalgamator was applied to fabricate the lipid nanoscaled UCAs. The morphology, diameter, polydispersity index(P.I), zeta potential and stability of nanoscaled UCAs were assessed. The enhancement effect of nanoscaled UCAs in vitro was also detected. Results The results of orthogonal test indicated that the oscillation time had a significant effect on the diameter of nanoscaled UCAs(P<0.05). The optimal condition of preparation was as follows: the amount of DPPA was 0.5 mL, oscillation time was 90 s, and centrifugation time was 3 min. The mean diameter and P.I of the nanoscaled UCAs fabricated under the optimal condition was(313.20±6.90)nm and 0.15, respectively. The Zeta potential was -12.40 mV. There was no significant difference in the enhancement effect between the nanoscaled UCAs and SonoVue in vitro(t=2.02, P>0.05). The basic physical characteristics of the nanoscaled UCAs remained unchanged after being stored in 4 ℃ refrigerator for 20 hours. Conclusion Nanoscaled UCAs with smaller diameter and good stability were successfully fabricated. All these characteristics indicate a promising future in targeted molecular imaging and drug or gene delivery.

Key words: Nanoscaled, Nanoparticle, Ultrasound contrast agents, Lipid, Enhancement effect

CLC Number: 

  • R445.1
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