Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (12): 23-28.doi: 10.6040/j.issn.1671-7554.0.2020.0817

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miR-203 targeting Survivin to inhibit the proliferation, migration and invasion of ovarian cancer cells

WANG Baojin1, ZHAO Xinxin1, LI Xia1, MA Qian1, WANG Xinyue1, SUN Yang2, SHI Zhongna1   

  1. 1. Department of Gynecology, The Third Affiliated Hospital of Zhengzhou University, Henan International Joint Laboratory of Ovarian Malignancies, Zhengzhou 450052, Henan, China;
    2. Department of Gynecology, Fujian Provincial Hospital, Fuzhou 350001, Fujian, China
  • Published:2020-12-08

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Abstract: Objective To investigate the effects of miR-203 targeting Survivin on the proliferation, migration and invasion of ovarian cancer cells SKOV3 and OVCAR3. Methods After miR-203 over-expressing group, Survivin over-expressing group and black control group of lentiviral vectors were constructed, the SKOV3 and OVCAR3 cells were transfected. After puromycin screening, the blank control group, miR-203 over-expressing group, Survivin over-expressing group, and miR-203 over-expressing plus Survivin group were established. The expressions of Survivin and epithelial-mesenchymal transition(EMT)-related proteins in each group were detected with Western blotting. The proliferation of cells was detected with MTT and plate clone formation assay. The cell migration and invasion were detected with Transwell assay. Results miR-203 over-expression inhibited the expressions of Survivin and EMT(P<0.05). MTT, plate clone formation and Transwell assay illustrated that, compared with the blank control group, the miR-203 over-expressing group had inhibited cell proliferation, migration, and invasion(P<0.05), while the Survivin over-expressing group reversed the inhibitory effects of miR-203(P<0.05). Conclusion miR-203 can inhibit EMT by targeting Survivin to inhibit the proliferation, migration, and invasion of ovarian cancer cells. The miR-203/Survivin/EMT axis is expected to be a new target for the treatment of ovarian cancer.

Key words: Ovarian cancer, miR-203, Survivin, Epithelial-mesenchymal transition

CLC Number: 

  • R737.31
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