JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 7-11.doi: 10.6040/j.issn.1671-7554.0.2015.025

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Effects of peptide YY on apoptosis of human pancreatic cancer cell line Miapaca-2 in vitro

LI Hongxia1, DONG Lei2, JIANG Jiong2, WANG Xinyang3   

  1. 1. Department of Gastroenterology, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China;
    2. Department of Gastroenterology, the Second Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China;
    3. Urological Research Laboratory, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China
  • Received:2015-01-08 Online:2015-12-10 Published:2015-12-10

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Abstract: Objectives To observe the effects of peptide YY(PYY)on inducing apoptosis of human pancreatic cancer cell line Miapaca-2. Methods The pancreatic cancer cell line Miapaca-2 was treated with PYY of different concentrations(0.01, 0.005, 0.0025 and 0.00125 mmol/L) for 48 h.The apoptosis and cell cycle were evaluated by flow cytometry, the expression of survivin mRNA was detected with RT-PCR, and the expressions of survivin and Caspase-3 protein were detected with Western blotting. Results After intervention with PYY for 48 h, celluar apoptosis rate was significantly increased in a dose-dependent manner. The cells of G0/G1 stage were significantly increased, but the cells of S and G2/M stages were significantly decreased. The hypodiploid apoptotic peak before the G1 stage was detected. PYY inhibited survivin protein and survivin mRNA expressions but increased Caspase-3 protein expression notably in a dose dependent manner(P<0.01). Conclusion PYY can induce apoptosis in a dose-dependent manner. PYY maybe block cell cyclein G0/G1 stage, downregulate survivin mRNA and protein expressions, increase Caspase-3 protein expression and finally induce apoptosis.

Key words: Peptide YY, Apoptosis, Pancreatic cancer cell, Survivin, Caspase

CLC Number: 

  • R735.9
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