JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 1-6.doi: 10.6040/j.issn.1671-7554.0.2014.991

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Effects of brain-derived neurotrophic factor on colon smooth muscle cells and the mechnism in mice

CAO Jing, CHEN Feixue, WANG Tengfei, ZHAO Hongyu, ZHAO Dongyan, ZUO Xiuli, LI Yanqing   

  1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-24 Online:2015-12-10 Published:2015-12-10

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Abstract: Objective To investigate the effects of brain-derived neurotrophic factor (BDNF) on the intracellular Ca2+ concentration ([Ca2+]i) alterations and smooth muscle α-actin (α-SMA) expression of smooth muscle cells (SMCs) in mice. Methods The α-SMA expression of colonic SMCs in the BDNF+/- mice was measured by Western blotting, and was compared with that in BDNF+/+ mice. The expression of tropomyosin-related kinase B (TrkB) receptor was identified in the primary colonic SMCs of the mice by immunofluorescence staining. After administration of BDNF and TrkB receptor antagonists (K252a), the expressions of α-SMA and TrkB-PLC-Ca2+ pathway were measured by Western blotting. The alteration of[Ca2+]i was measured by[Ca2+]i imaging. Results The expression of α-SMA was obviously decreased in BDNF+/- mice compared with that in BDNF+/+ mice. The TrkB receptor was identified in the SMCs. After administration of BDNF, the expressions of α-SMA, TrkB-PLC-Ca2+ signal pathway and[Ca2+]i increased. K252a could reverse those changes. Conclusion BDNF might induce the alterations of[Ca2+]i and α-SMA expression of SMC by TrkB-PLC-Ca2+ signal pathway, which might be the mechanism to affect the gut motility.

Key words: TrkB-PLC-Ca2+ signal pathway, Gut motility, Brain-derived neurotrophic factor, Smooth muscle cells

CLC Number: 

  • R574
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