JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.1340

    Next Articles

Brain-derived neurotrophic factor modulates intestinal barrier integrity in mice

ZHAO Dongyan, YU Yanbo, ZUO Xiuli   

  1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-12-25 Online:2016-07-10 Published:2016-07-10

PDF (PC)

380

Abstract: Objective To investigate the effects of brain-derived neurotrophic factor(BDNF)on intestinal barrier integrity in mice. Methods Ileal tissues and colonic tissues from BDNF+/+ mice and BDNF+/- mice were prepared. The intestinal epithelial structure was detected by transmission electron microscopy. Expressions and distributions of tight junction proteins(occludin and ZO-1)in the colonic mucosa were evaluated by immunohistochemistry. Expressions of tight junction proteins(claudin-1 and claudin-2)in the colonic mucosa were evaluated by Western blotting. Results Compared with BDNF+/+ mice, BDNF+/- mice displayed integral ileal epithelial barrier and impaired colonic mucosa integrity which was characterized by diminished microvilli, widened intercellular space and bacteria invasion in the colonic mucosa. BDNF+/- mice also exhibited increased expression of claudin-2 and decreased expression of occludin, ZO-1 and claudin-1 in the colonic mucosa. Conclusion BDNF may play a role in regulating the colonic epithelial barrier in mice.

Key words: Brain-derived neurotrophic factor, Intestinal barrier, Mice, Tight junction proteins

CLC Number: 

  • R574
[1] Yu YB, Zuo XL, Zhao QJ, et al. Brain-derived neurotrophic factor contributes to abdominal pain in irritable bowel syndrome[J]. Gut, 2012, 61(5): 685-694.
[2] Donovan MJ, Lin MI, Wiegn P, et al. Brain derived neurotrophic factor is an endothelial cell survival factor required for intramyocardial vessel stabilization[J]. Development, 2000, 127(21): 4531-4540.
[3] Matsuda S, Fujita T, Kajiya M, et al. Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1β and tumor necrosis factor-α[J]. J Periodontal Res, 2015, 50(4): 444-451.
[4] Quadri SK. Cross talk between focal adhesion kinase and cadherins: Role in regulating endothelial barrier function[J]. Microvasc Res, 2012, 83(1): 3-11.
[5] Cunningham KE, Turner JR. Myosin light chain kinase: pulling the strings of epithelial tight junction function[J]. Ann N Y Acad Sci, 2012, 1258(1): 34-42.
[6] Shen L. Tight junctions on the move: molecular mechanisms for epithelial barrier regulation[J]. Ann N Y Acad Sci, 2012, 1258(1): 9-18.
[7] 陈飞雪, 于岩波, 王鹏, 等. 脑源性神经营养因子对小鼠回肠及结肠平滑肌收缩活动的影响及其机制[J]. 山东大学学报(医学版), 2012, 50(4): 42-46. CHEN Feixue, YU Yanbo, WANG Peng, et al. Brain-derived neurotrophic factor influences contractile activity in the isolated ileum and colon of mice[J]. Journal of Shandong University(Health Science), 2012, 50(4): 42-46.
[8] Vijendravarma RK, Narasimha S, Chakrabarti S, et al. Gut physiology mediates a trade-off between adaptation to malnutrition and susceptibility to food-borne pathogens[J]. Ecol Lett, 2015, 18(10): 1078-1086.
[9] Musch MW, Clarke LL, Mamah D, et al. T cell activation causes diarrhea by increasing intestinal permeability and inhibiting epithelial Na+/K+-ATPase[J]. J Clin Invest, 2002, 110(11): 1739-1748.
[10] Gecse K, Roka R, Ferrier L, et al. Increased faecal serine protease activity in diarrhoeic IBS patients: a colonic lumenal factor impairing colonic permeability and sensitivity[J]. Gut, 2008, 57(5): 591-599.
[11] Rauhavirta T, Lindfors K, Koskinen O, et al. Impaired epithelial integrity in the duodenal mucosa in early stages of celiac disease[J]. Transl Res, 2014, 164(3): 223-231.
[12] Annaházi A, Ferrier L, Bézirard V, et al. Luminal cysteine-proteases degrade colonic tight junction structure and are responsible for abdominal pain in constipation-predominant IBS[J]. Am J Gastroenterol, 2013, 108(8): 1322-1331.
[13] Erickson JT, Conover JC, Borday V, et al. Mice lacking brain-derived neurotrophic factor exhibit visceral sensory neuron losses distinct from mice lacking NT4 and display a severe developmental deficit in control of breathing[J]. J Neurosci, 1996, 16(17): 5361-5371.
[14] Amasheh S, Fromm M, Günzel D. Claudins of intestine and nephron-a correlation of molecular tight junction structure and barrier function[J]. Acta Physiol, 2011, 201(1): 133-140.
[15] Shen L, Weber CR, Raleigh DR, et al. Tight junction pore and leak pathways: a dynamic duo[J]. Annu Rev Physiol, 2011, 73: 283-309.
[16] Zhang YG, Wu S, Xia Y, et al. Salmonella infection upregulates the leaky protein claudin-2 in intestinal epithelial cells[J]. PLoS one, 2013, 8(3): e58606. doi: 10.1371/journal.pone.0058606.
[17] Rosenthal R, Milatz S, Krug SM, et al. Claudin-2, a component of the tight junction, forms a paracellular water channel[J]. J Cell Sci, 2010, 123(11): 1913-1921.
[18] Alan SL, Cheng MH, Coalson RD. Calcium inhibits paracellular sodium conductance through claudin-2 by competitive binding[J]. J Biol Chem, 2010, 285(47): 37060-37069.
[19] Martínez C, Lobo B, Pigrau M, et al. Diarrhoea-predominant irritable bowel syndrome: an organic disorder with structural abnormalities in the jejunal epithelial barrier[J]. Gut, 2013, 62(8): 1160-1168.
[1] DUAN Chu-Hong, LIU Kai, YIN Hai-Yan, ZHAO Shi-Dou, LIU Feng-Tao. Effects of lectin receptors of WGA,RCA and ECL on the development of cleft palate induced by excess retinoid acid in KM mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 47-.
[2] ZHANG Wenxue, XU Lidong, ZHANG Mingming, GUO Chuanguo, ZUO Xiuli. NR2B contributes to visceral hypersensitivity in irritable bowel syndrome via mBDNF [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 17-22.
[3] JIAO Fengping, WANG Yu, YU Ailian, TIAN Zhaoju, YANG Shulin. Immunogenicity of the eukaryotic expression vector pcDNA3.1(-)-P6-gBCTL-TBK-1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 75-78.
[4] ZHENG Qianqian, LIU Ti, XIE Keqin, LIN Yi, LI Zhong, WANG Xianjun, BI Zhenqiang, WEN Hongling, SONG Yanyan, ZHAO Li. Anti-influenza virus activities of garlic oil in mice model in vivo [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 23-27.
[5] WEI Xiujuan, WU Xiuyin, TONG Dongdong, LI Jing, YANG Xiaolu, ZHANG Fenghe. Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 50-54.
[6] YUAN Xiuyu, DONG Yuanjun, LIANG Xia, HU Min, ZHANG Guiqing. Effect of post-traumatic stress on hippocampal BDNF expression in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 37-41.
[7] LI Maoyu, TANG Shi, DU Yifeng. Expression and significance of TRB3 in the brain of APP/PS1 transgenic mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 1-5.
[8] YANG Qianqian, SUN Gaoying, CAO Zhixin, YIN Haiyan, WANG Haibo, LI Jianfeng. Expression of NLRX1 in cochlear hair cells of C57BL/6 mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 11-16.
[9] WANG Jinghan, LI Xiaofei, ZHANG Zhibing, WANG Haibo, LI Jianfeng. Prestin expression in outer hair cells of Spag6 gene knockout mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 16-22.
[10] LIANG Yu, XIN Tao, LIU Qian, ZHENG Xiangrong, YANG Yihang, PANG Qi. Effects of theanine on the proliferation and differentiation of neural stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 49-54.
[11] ZHOU Xiaoyan, LI Ming, GUO Jing, ZUO Xiuli, LI Yanqing. Mucus fills the intestinal epithelial gaps and maintains the barrier function [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 62-66,80.
[12] CAO Jing, CHEN Feixue, WANG Tengfei, ZHAO Hongyu, ZHAO Dongyan, ZUO Xiuli, LI Yanqing. Effects of brain-derived neurotrophic factor on colon smooth muscle cells and the mechnism in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 1-6.
[13] OUYANG Bing, PENG Zhongtian, WANG Pei. Expression and significance of cannabinoid receptor 1 and FAK in hepatic tissues of schistosomal hepatic fibrosis mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 11-14.
[14] DUAN Yuwei, XI Yanwei, YANG Xiaoye, DU Hongliang, ZHAI Guangxi . Preparation of curcumin-loaded bile salt/phospholipid mixed micelles and its physicochemical properties [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 63-67.
[15] XU Yihuai1, SUN Shuzhen1, ZHEN Junhui2, LI Qian1, WANG Cong1. Roles of CXCL16 and ox-LDL in mice with adriamycin-induced nephropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 68-72.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd