Abstract: Objective To investigate the immune effects of herpes simplex virus type 2(HSV-2 )DNA vaccine of pc(pcDNA3.1-)-P6-gBCTL-TBK-1 in BALB/c mice. Methods The eukaryotic expression plasmid of pc-P6-gBCTL-TBK-1 was constructed and transfected into HeLa cells, and the expressed protein was verified by Western blotting. The BALB/c mice were immunized by the plasmids through intramuscular injection for three times, in week 0, 2 and 4. In week 5, the levels of interferon(IFN-γ), interleukin-18(IL-18), interleukin-4(IL-4), interleukin-6(IL-6)and specific HSV-2gD-P6 IgG antibody were measured by ELISA. In week 8, spleen cells of vaccinated mice were separated and the proliferation of spleen lymphocytes were detected by MTT. The activity of cytotoxic T lymphocyte(CTL)was detected by lactic dehydrogenase(LDH)method. Results After being inoculated by the plasmid of pc-P6-gBCTL-TBK-1, the mice were induced to generate higher level of specific antibody, including IFN-γ, IL-18 and IL-4; the CTL kill cells activity and proliferation capability of splenic lymphocytes were improved. Conclusion The recombinant plasmid of pc-P6-gBCTL-TBK-1 has immunogenicity, which can induce the humoral and cellular immune responses, and TBK-1 may have good function of molecular chaperone.

Key words: Herpes simplex virus type 2, DNA vaccine, TANK Binding Kinase-1, Mice, Inbred BALB C