JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 11-14.doi: 10.6040/j.issn.1671-7554.0.2014.097

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Expression and significance of cannabinoid receptor 1 and FAK in hepatic tissues of schistosomal hepatic fibrosis mice

OUYANG Bing1, PENG Zhongtian2, WANG Pei3   

  1. 1. Department of Infectious Diseases, Ninth Hospital of Nanchang, Nanchang 330002, Jiangxi, China;
    2. Department of Infectious Diseases, First Affiliated Hospital of University of South China, Hengyang 421001, Hu'nan, China;
    3. Department of Infectious Diseases, First People's Hospital of Huaihua City, Huaihua 418000, Hu'nan, China
  • Received:2014-02-26 Revised:2014-09-04 Online:2014-09-10 Published:2014-09-10
  • Contact: 彭忠田。E-mail:hncspzt@yahoo.com.cn E-mail:hncspzt@yahoo.com.cn

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Abstract: Objective To investigate the expression and significance of cannabinoid receptor 1 (CB1) and focal adhesion kinase (FAK) in hepatic tissues of schistosomal hepatic fibrosis mice. Methods A total of 32 Kunming mice were randomly divided into two groups: normal control group (n=10) and model group (n=22). The schistosome-induced liver fibrosis models were established by attaching cercaria to the skin on the ventral side of the mice and allowing infection to occur via direct penetration. All mice were raised normally, and at week 8 all mice were sacrificed to gain hepatic tissue samples. Mice in the model group were divided into 3 subgroups according to the severity of hepatic fibrosis. The pathological changes were determined by HE staining, the degrees of fibrosis were examined by Masson staining, the expression of CB1 was detected by immunohistochemical method, and the expressions of FAK mRNA and CB1 mRNA were tested by reverse transcription polymerase chain reaction (RT-PCR). Results In the model group, significant schistosome egg granuloma and hepatic fibrosis were observed, and CB1, CB1 mRNA and FAK mRNA were detected in all subgroups. Besides, with the aggravation of fibrosis, the expressions of CB1, CB1 mRNA and FAK mRNA increased. Conclusion CB1 and FAK play an important role in the genesis and progression of schistosomal hepatic fibrosis.

Key words: Focal adhesion kinase, Schistosomal hepatic fibrosis, Cannabinoid receptor-1, Mice

CLC Number: 

  • R381
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