JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 50-54.doi: 10.6040/j.issn.1671-7554.0.2015.1222

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Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma

WEI Xiujuan1, WU Xiuyin2, TONG Dongdong1, LI Jing1, YANG Xiaolu1, ZHANG Fenghe1   

  1. 1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Peoples Hospital of Laiwu City, Laiwu 271100, Shandong, China
  • Received:2015-12-03 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To investigate the expressions of brain-derived neurotrophic factor(BDNF)and its primary receptor tyrosine kinase receptor B(TrkB)in tongue squamous cell carcinoma(TSCC), and to explore the effect of BDNF on the proliferation of TSCC. Methods The expressions of BDNF and TrkB in 50 TSCC and 13 normal mucosa tissues were detected with immunohistochemical method. The expressions of BDNF and TrkB in human tongue squamous cell carcinoma cell lines(Tca8113)were examined with immunofluorescence technique. Cell viability of Tca8113 was detected with CCK8 assay. Results The positive rate of staining of BDNF and TrkB in TSCC was significantly higher than that in normal tongue mucosa(P<0.05). In addition, the rate of positive expression was related to infiltration depth and lymph node metastasis. BDNF(200 ng/mL)significantly promoted the proliferation of Tca8113. Conclusion BDNF and TrkB may be closely associated with the genesis and development of TSCC. Moreover, BDNF may promote cell proliferation of TSCC.

Key words: Brain-derived neurotrophic factor, Tyrosine kinase receptor B, Tongue squamous cell carcinoma, Proliferation ability

CLC Number: 

  • R782
[1] Lin CY, Chen HJ, Li TM, et al. β5 Integrin up-regulation in brain-derived neurotrophic factor promotes cell motility in human chondrosarcoma[J]. PLoS One, 2013, 8(7): e67990. doi: 10.1371/journal.pone.0067990.
[2] Zhang S, Guo D, Luo W, et al. TrkB is highly expressed in NSCLC and mediated BDNF-induced the activation of Pyk2 signaling and the invasion of A549 cells[J]. BMC Cancer, 2010, 10: 43. doi: 10.1186/1471-2407-10-43.
[3] 刘建英, 李坤霞, 李爱敏, 等. TrkB-BDNF信号传导通路对神经母细胞瘤细胞耐药和转移的影响[J]. 中华小儿外科杂志, 2013, 34(8): 607-612. LIU Jianying, LI Kunxia, LI Aimin, et al. Effects of TrkB-BDNF signaling pathway on chemoresistance and metastasis of neuroblastoma cells[J]. Chin J Pediatr Surg, 2013, 34(8):607-612.
[4] Li Z, Oh DY, Nakamura K, et al. Perifosine-induced inhibition of Akt attenuates brain-derived neurotrophin factor /TrkB-induced chemoresistance in neuroblastoma in vivo[J]. Cancer, 2011, 117(23):5412-5422.
[5] 郑家伟, 李金忠, 钟来平, 等. 口腔鳞状细胞癌临床流行病学研究现状[J]. 中国口腔颌面外科杂志, 2007, 5(2):83-90. ZHENG Jiawei, LI Jinzhong, ZHONG Laiping, et al.Clinical epidemiology and risk factors of oral squamous cell carcinoma: an overview[J]. China Journal of Oral and Maxillofacial Surgery, 2007, 5(2):83-90.
[6] Ng YK, Wong EY, Lau CP, et al. K252a induces anoikissensitization with suppression of cellular migration in Epstein-Barr virus(EBV)-associated nasopharyngeal carcinoma cells[J]. Invest New Drugs, 2012, 30(1):48-58.
[7] Sclabas GM, Fujioka S, Schmidt C, et al. Overexpression of tropomysin-related kinase B in metastatic human pancreatic cancer cells[J]. Clin Cancer Res, 2005, 11(2Pt1):440-449.
[8] Odate S, Nakamura K, Onishi H, et al. TrkB/BDNF signaling pathway is a potential therapeutic target for pulmonary larger cell neuroendocrine carcinoma[J]. Lung Cancer, 2013, 79(3):205-214.
[9] Au CW, Siu MK, Liao X, et al. Tyrosine kinase B receptor and BDNF expression in ovarian cancers-Effect on cell migration, angiogenesis and clinical outcome[J]. Cancer Lett, 2009, 281(2):151-161.
[10] Bronzetti E, Artico M, Forte F, et al. A possible role of BDNF in prostate cancer detection[J]. Oncol Rep, 2008, 19(4):969-974.
[11] 刘天卿, 任海军, 温爽, 等. 胃癌组织中Survivin、TrkB和BDNF的表达及意义[J]. 临床与实验病理学杂志, 2009, 25(3):291-294. LIU Tianqing, REN Haijun, WEN Shuang, et al. Expression and significance of survivin, TrkB and BDNF in gastric carcinoma[J]. Clin Exp Pathol, 2009, 25(3):291-294.
[12] Douma S, Van Laar T, Zevenhoven J, et al. Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB[J]. Nature, 2004, 430(3):1034-1039.
[13] Yang ZF, Ho DW, Lam CT, et al. Identification of brain-derived neurotrophic factor as a novel functional protein in hepatocellular carcinoma[J]. Cancer Res, 2005, 65(1):219-225.
[14] 孙春艳, 胡豫, 郭涛, 等. 脑源性神经营养因子激活其受体TrkB在多发性骨髓瘤发病机制中的作用[J]. 中华血液学杂志, 2008, 29(4):238-242. SUN Chunyan, HU Yu, GUO Tao, et al. Study of brain-derived neurotrophic factor activating TrkB signaling cascades in the pathogenesis of multiple myeloma[J]. Clin J Hematol, 2008, 29(4):238-242.
[15] Hu Y, Sun CY, Wang HF, et al. Brain-derived neurotrophic factor promotes growth and migration of multiple myeloma cells[J]. Cancer Genet Cytoqenet, 2006, 169(1):12-20.
[16] Serres F, Carney SL. Nicotine regulates SH-SY5Y neuroblastoma cell proliferation through the release of brain-derived neurotrophic factor[J]. Brain Res, 2006, 1101(1):36-42.
[17] Miknyoczki SJ, Wan W, Chang H, et al. The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice[J]. Clin Cancer Res, 2002, 8(6):1924-1931.
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