p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达

doi:10.6040/j.issn.1671-7554.0.2015.1223

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 12-16.doi: 10.6040/j.issn.1671-7554.0.2015.1223

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p38 MAPK signaling pathway regulates the expressions of type Ⅰ and Ⅲ collagens in human aortic smooth muscle cells

WANG Xiaolin¹, ZHOU Yuanli², SUN Wei³, LI Li¹

1. Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
2. Department of Health, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
3. School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China

Received:2015-12-03 Online:2016-08-10 Published:2016-08-10

Abstract

Abstract: Objective To investigate the effects of p38 MAPK on the expressions of type Ⅰ and type Ⅲ collagens in human aortic smooth muscle cells(HASMCs). Methods HASMCs were treated with inhibitors and siRNAs respectively. Inhibitor test cells were divided into 3 groups: (1) normal control group(cells were cultured in smooth muscle cell complete medium); (2) DMSO control group(cells were treated with 0.01% DMSO); (3) p38 MAPK inhibitor group(cells were treated with 0.01% p38 MAPK inhibitor(20 mg/mL). Gene silencing test cells were divided into 3 groups: (1) static control group(cells were cultured in smooth muscle cell complete medium); (2) negative control siRNA group(cells were transfected with negative control siRNAs for 48 h using MACSfectin reagent); (3) p38 MAPK siRNA group(cells were transfected with p38 MAPK siRNAs for 48 h using MACSfectin reagent). The mRNA expressions 山东大学学报 (医学版)54卷8期 -王晓琳,等.p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达 \=- of type Ⅰ and Ⅲ collagens were detected by qRT-PCR, and the protein expressions were detected by Western blotting. The siRNAs transfection efficiency was observed with fluorescence microscope. Results Compared with the normal control group, the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the DMSO control group(P>0.05), while the expressions decreased significantly in the p38 MAPK inhibitor group(P<0.05). Compared with the static control group, the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the negative control siRNA group(P>0.05), while the expressions decreased significantly in the p38 MAPK siRNA group(P<0.05). Conclusion p38 MAPK signaling pathway regulates the expressions of type Ⅰ and type Ⅲ collagens in HASMCs.

Key words: Type Ⅲ collagen, Human aortic smooth muscle cells, p38 MAPK, Type Ⅰ collagen

CLC Number:

R541.4

WANG Xiaolin, ZHOU Yuanli, SUN Wei, LI Li. p38 MAPK signaling pathway regulates the expressions of type Ⅰ and Ⅲ collagens in human aortic smooth muscle cells[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 12-16.

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