JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 34-38.doi: 10.6040/j.issn.1671-7554.0.2014.102

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Inhibiting Effect of ginsenoside Re on the expression of PARP-1 in human umbilical vein endothelial cell

CHEN Jianhua1, ZHU Banghao2   

  1. 1. Department of Pharmacology, School of Pharmacy, North Sichuan Medical University, Nanchong 637000, Sichuan, China;
    2. Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510000, Guangdong, China
  • Received:2014-02-27 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 朱邦豪。E-mail:zhubh@mail.sysu.edu.cn E-mail:zhubh@mail.sysu.edu.cn

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Abstract: Objective To screen herbal monomers with potential inhibition on poly(ADP-ribose)polymerase-1 (PARP-1), and to study their inhibiting effects on PARP-1 expression in human umbilical vein endothelial cell (HUVEC). Methods Six herbal monomers which might have potential inhibiting effect on PARP-1 were screened by computer visual screening in Chinese Natural Product Database. The cells were divided into the following groups:high (1×10-4 mol/L) and low (1×10-6 mol/L) doses groups for the herbal monomers, the 3-aminobenzamide(3-AB) positive control group and model group. Western blotting was used to test the expression of PARP-1. When the tested drugs exhibited inhibiting effects on PARP-1 expression, the drug-dependent experiments would be carried out with the drug concentration being 1×10-4 to 1×10-8 mol/L. Results 1×10-4 and 1×10-6 mol/L ginsenoside Re and 1×10-4 mol/L ginsenoside Rb1 had inhibiting effect on PARP-1 activated by ONOO-. 1×10-4 to 1×10-8 mol/L ginsenoside Re had obvious inhibiting effect on PARP-1 expression in a dose-dependent manner (P<0.05). Conclusion Ginsenoside Re can inhibit protein expression of PARP-1 activated by ONOO- on HUVECs and has protecting effect on ONOO--induced injury in a dose-dependent manner.

Key words: Human umbilical vein endothelial cell, Herbal monomer, Poly(ADP-ribose)polymerase-1, ONOO-

CLC Number: 

  • R965.1
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