Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (11): 48-52.doi: 10.6040/j.issn.1671-7554.0.2021.0795

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Correlation among PAX1 methylation, high-grade cervical intraepithelial lesions and HR-HPV classification

LIANG Tingting, YANG Yongxia, HOU Congzhe, HUANG Taisheng, WANG Huali, ZHU Lin   

  1. Department of Gynecology, The Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Published:2021-11-11

Abstract: Objective To analyze the correlation among PAX1 methylation, cervical high-grade intraepithelial neoplasia and the type of high-risk HPV. Methods From Aug. 2019 to Oct. 2020, women who underwent thinprep cytologic test(TCT)and high-risk human papillomavirus(HR-HPV)DNA examinations in The Second Hospital of Shandong University were screened, and 397 patients with abnormal TCT results: ≥atypical squamous cells of undetermined significance(ASCUS), and/or HR-HPV positive were selected. According to the consensus of Chinese experts on the application of colposcopy in 2020, those with abnormal colposcopy results should also undergo biopsy and RT-qPCR to detect PAX1 methylation. Results Pathological grade of 397 patients were CIN3+(55 cases), normal/cervicitis(72 cases), CIN1(36 cases), and CIN2(72 cases); their PAX1 methylation levels were 18.64(4.01, 20.83), 20.22(19.24, 20.84), 20.33(19.65, 21.00), and 20.43(15.31, 21.08). The PAX1 methylation level of CIN3+ patients was higher than that of normal/CIN1/CIN2 patients(Z=3.03, 2.65, 3.06; P=0.002, 0.008, 0.002). The PAX1 methylation levels of HPV16-positive patients(89 cases)and type 12 high-risk HPV(12HR-HPV)-positive patients(174 cases)were 19.72(10.00, 20.91)and 21.00(18.97, 21.02)(Z=2.736, P=0.006). Among ASCUS patients, the methylation levels of CIN2+(23 cases)and normal/CIN1(19 cases)were 20.47(19.33, 21.18)and 18.03(11.2, 20.82)(Z=2.45, P=0.014). Conclusion The degree of PAX1 methylation is related to high-grade cervical intraepithelial lesions and HPV types. PAX1 methylation detection would benefit the early identification of CIN3+ and high-grade cervical lesions in ASCUS, help the diversion of ASCUS, and reduce the referral rate of colposcopy.

Key words: PAX1 methylation, Cervical cancer, Cervical intraepithelial lesions, Atypical squamous cells of undetermined significance, High-risk HPV

CLC Number: 

  • R737.33
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