Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 103-109.doi: 10.6040/j.issn.1671-7554.0.2018.590

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Effect of IQ motif-containing GTPase activating protein 3 on the proliferation and migration of cervical cancer cells

WANG Yong1, JIANG Chen2, ZHOU Shiying1, YANG Xiaomei3   

  1. 1. Department of Obstetrics, Jinan Maternity and Child Care Hospital, Jinan 250002, Shandong, China;
    2. Department of Gynaecology, Jinan Maternity and Child Care Hospital, Jinan 250002, Shandong, China;
    3. Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2018-05-20 Published:2022-09-27

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Abstract: Objective To investigate the expression of IQ motif-containing GTPase activating protein 3(IQGAP3)in the cervical cancer tissues and cell lines, and its subsequent influences on cell proliferation, apoptosis and migration. Methods The expression of IQGAP3 was analyzed by immunohistochemistry and real-time PCR in cervical cancer and adjacent normal tissues. The siRNA targeting IQGAP3 was used to knockdown the expression of IQGAP3. The influences of IQGAP3 silencing on cancer cell proliferation, apoptosis and migration were determined by MTT methods, flow cytometry and transwell migration assay, respectively. Results The expression of IQGAP3 was up-regulated in the cervical cancer tissues and cervical cancer cell lines. The up-regulation of IQGAP3 was associated with the poor prognosis of patients with cervical cancer(χ2=6.15, P=0.01). Down-regulation of IQGAP3 decreased proliferation and promoted apoptosis in CaSki and HeLa cells. Moreover, as compared with siScramble transfected cells, the number of migrating cell was significantly decreased in CaSki and HeLa cells due to si-IQGAP3 transfection. In addition, Western blotting analysis showed that knockdown of IQGAP3 increased the expression of epithelial marker E-cadherin and decreased the 山 东 大 学 学 报 (医 学 版)56卷10期 -王永,等.IQ结构域GTP酶激活蛋白3表达对宫颈癌细胞增殖和转移的影响 \=-expression of mesenchymal marker N-cadherin(all P<0.001). Conclusion IQGAP3 is up-regulated in cervical cancer tissues and cells, and its ver-expression promots cell proliferation and the migration capacity in cervical cancer cells. IQGAP3 plays a key role in cervical cancer progression and could be a potential therapeutic target for cervical cancer.

Key words: IQ motif-containing GTPase activating protein 3, Cervical cancer, Cell proliferation, Cell apoptosis, Epithelial-mesenchymal transition

CLC Number: 

  • R737.33
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