Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (1): 34-39.doi: 10.6040/j.issn.1671-7554.0.2021.0274

Previous Articles     Next Articles

Expressions and significance of CDC7 and MCM4 in 105 cases of cervical lesions

CHEN Quancai1,2, HAN Sai1, LIU Lu1,2, SUN Yu1,2, YOU Xuewu1,2, ZHANG Junhua1,2, ZHANG Youzhong1,2   

  1. 1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-01-08

PDF (PC)

274

Abstract: Objective To explore the expressions and clinical significance of cell division cycle 7(CDC7)and minichromosome maintenance protein 4(MCM4)in cervical cancer and precancerous lesions. Methods The expressions of CDC7 and MCM4 in 30 cases with normal cervix, 30 cases with grade I cervical intraepithelial neoplasia(CINⅠ), 25 cases with CIN Ⅱ-Ⅲ, and 50 cases with cervical cancer were detected with immunohistochemistry. The expressions were compared with Ki67, and their correlation with the clinicopathological characteristics of cervical cancer was analyzed. Results (1) The positive expression rate of CDC7 in normal cervical tissues, CINⅠ, CINⅡ-Ⅲ, and cervical cancer tissues was 6.7%, 26.7%, 56.0% and 78.0%, respectively, which was moderately and positively correlated with the lesion degree(rs=0.625, P<0.001). The positive expression rate of MCM4 in the 4 groups was 26.7%, 36.7%, 64.0% and 88.0%, respectively, which was moderately and positively correlated with the lesion degree(rs=0.680, P<0.001). The positive expression rate of Ki67 in the 4 groups was 10.0%, 26.7%, 60.0% and 84.0%, respectively, which was moderately and positively correlated with the lesion degree(rs=0.660, P<0.001). (2) The positive expression level of CDC7 was correlated with the pathological grade, lymphatic metastasis and depth of muscular invasion of cervical cancer(P=0.024, P=0.033, P=0.023). The positive expression level of MCM4 was correlated with the pathological grade and depth of muscular invasion of cervical cancer(P=0.010, P=0.003). The positive expression level of Ki67 was correlated with the pathological grade, lymphatic metastasis and depth of muscular invasion of cervical cancer(P=0.009, P=0.016, P=0.021). (3) The expression levels of CDC7 and MCM4 were positively correlated in cervical cancer tissues(P=0.001). (4) After multivariate adjustment, the positive expressions of CDC7, MCM4 and Ki67 were risk factor for the severity of cervical lesions. Conclusion CDC7 and MCM4 are highly expressed in CIN and cervical cancer, which suggests that they have potential clinical value as reference indicators for the diagnosis and treatment of CIN and cervical cancer.

Key words: Cervical cancer, Cervical intraepithelial neoplasia, Cell division cycle 7, Minichromosome maintenance protein 4, Antigen Ki67

CLC Number: 

  • R737.33
[1] 余艳琴, 郝金奇, 徐慧芳, 等. 宫颈癌综合预防和控制的应用和前景[J]. 中国医学科学院学报, 2020, 42(4): 535-539. YU Yanqin, HAO Jinqi, XU Huifang, et al. Multidisciplinary prevention and control of cervical cancer: application and prospects [J]. Acta Academiae Medicinae Sinicae, 2020, 42(4): 535-539.
[2] 包克勇, 陶晓玉, 张雪, 等. KLF12在宫颈癌中的表达及其调控IL-6/STAT3通路影响宫颈癌细胞增殖和凋亡的机制研究[J]. 中国免疫学杂志, 2021, 37(16): 1979-1983. BAO Keyong, TAO Xiaoyu, ZHANG Xue, et al. Research on KLF12 expression in cervical cancer and its mechanism of effect on proliferation and apoptosis of cervical cancer cells by regulating IL-6/STAT3 pathway [J]. Chinese Journal of Immunology, 2021, 37(16): 1979-1983.
[3] Li XM, Luo XG, He JF, et al. Induction of apoptosis in human cervical carcinoma HeLa cells by active compounds from Hypericum ascyron L [J]. Oncol Lett, 2018, 15(3): 3944-3950.
[4] 仲君, 张彭南. 阴道微生态与宫颈HPV感染、宫颈病变的关系[J]. 现代妇产科进展, 2021, 30(9): 703-705, 709.
[5] Basu P, Banerjee D, Mittal S, et al. Sensitivity of APTIMA HPV E6/E7 mRNA test in comparison with hybrid capture 2 HPV DNA test for detection of high risk oncogenic human papillomavirus in 396 biopsy confirmed cervical cancers [J]. Journal of Medical Virology, 2016, 88(7): 1271-1278.
[6] 孙泽雨, 陈颖, 林家香, 等. 磷脂酰肌醇-4-磷酸酶Ⅱ型对宫颈癌细胞增殖及凋亡的影响[J]. 山东大学学报(医学版), 2017, 55(11): 1-6. SUN Zeyu, CHEN Ying, LIN Jiaxiang, et al. Influence of inositol polyphosphate-4-phosphatase type Ⅱ on the proliferation and apoptosis of cervical cancer cells [J]. Journal of Shandong University(Health Science), 2017, 55(11): 1-6.
[7] 李帆, 郝崔培. HPV分型检查配合TCT检测在宫颈病变早期筛查效果[J]. 中国计划生育学杂志, 2021, 29(5): 1069-1071, 1076. LI Fan, HAO Cuipei. Efficacy of HPV typing test combined with TCT for screening cervical lesions early [J]. Chinese Journal of Family Planning, 2021, 29(5): 1069-1071, 1076.
[8] 王海燕, 丁燕. CDC7蛋白在宫颈病变组织中的阳性表达率和临床意义[J]. 中国妇幼保健, 2020, 35(12): 2323-2325.
[9] Wang X, Yukio Ishimi. Function of the amino-terminal region of human MCM4 in helicase activity [J]. J Biochem, 2018, 164(6): 449-460.
[10] 舒锦, 周琦. FIGO 2018子宫颈癌新分期变化与思考[J]. 中国实用妇科与产科杂志, 2019, 35(12): 1395-1400.
[11] 贾宇, 陈彦, 刘建生. MCM 基因家族在肝细胞癌中生存预测价值的分析[J]. 中国普外基础与临床杂志, 2021, 28(7): 898-903. JIA Yu, CHEN Yan, LIU Jiansheng. Analysis of the survival prediction value of MCM gene family in hepatocellular carcinoma [J]. Chinese Journal of Bases and Clinics in General Surgery, 2021, 28(7): 898-903.
[12] Sasi NK, Coquel F, Lin YL, et a1. DDK has a primary role in processing stalled replication forks to initiate downstream checkpoint signaling [J]. Neoplasia, 2018, 20(10): 985-995.
[13] Wang YF, Chen HR, Zhang JL, et al. MCM family in gastrointestinal cancer and other malignancies: From functional characterization to clinical implication [J]. Biochim Biophys Acta Rev Cancer, 2020, 1874(2): pp188415. doi: 10. 1016/J.bbcan. 2020. 188415.
[14] 李晨红, 宋长丰, 林志康, 等. 蛇葡萄素对结肠癌细胞增殖抑制、自噬诱导及自噬对增殖的影响研究[J]. 四川师范大学学报(自然科学版), 2021, 44(3): 390-397.
[15] 毛红美, 孙毅. 原纤毛及其在肿瘤发生发展中的作用[J]. 浙江大学学报(医学版), 2021, 50(2): 245-260. MAO Hongmei, SUN Yi. Primary cilium and its role in tumorigenesis [J]. Journal of Zhejiang University(Medical Sciences), 2021, 50(2): 245-260.
[16] 张从义, 崔逸峰, 李宝宝, 等. 细胞分裂周期7蛋白在肝癌组织的表达及其对肝癌细胞增殖和侵袭的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 150-155. ZHANG Congyi, CUI Yifeng, LI Baobao, et a1. Expression of cell division cycle 7 protein in hepatocellular carcinoma tissues and its effect on proliferation and invasion of hepatocellular carcinoma cells [J]. Practical Oncology Journal, 2020, 34(2): 150-155.
[17] Jaafari-Ashkavandi Z, Ashraf MJ, Abbaspoorfard AA. Overexpression of CDC7 in malignant salivary gland tumors correlates with tumor differentiation [J]. Brazi Otorhinolaryngol, 2019, 85(2): 144-149.
[18] Cao JX, Lu Y. Targeting CDC7 improves sensitivity to chemotherapy of esophageal squamous cell carcinoma [J]. Onco Targets Ther, 2019, 12: 63-74. doi: 10.2147/OTT.S183629.
[19] Zheng RN, Lai GW, Li RF, et al. Increased expression of MCM4 is associated with poor prognosis in patients with hepatocellular carcinoma [J]. J Gastrointes Oncol, 2021, 12(1):153-173.
[20] 刘宾, 刘辉, 李宝玉. 微小染色体维持蛋白4在胰腺导管腺癌中的表达及其与预后相关性研究 [J]. 国际生物医学工程杂志, 2021, 44(1): 28-33. LIU Bin, LIU Hui, LI Baoyu. Expression of minichromosome maintenance protein 4 in pancreatic ductal adenocarcinoma and its correlation with prognosis [J]. International Journal of Biomedical Engineering, 2021, 44(1): 28-33.
[21] Li ZG, Chen JH, Zhao SB, et al. Discovery and validation of novel biomarkers for detection of cervical cancer [J]. Cancer Medicine, 2021, 10(6): 2063-2074.
[22] Ramesh A, Chander RV, Srinivasan C, et al. Prevalence of angiogenesis, proliferation, and apoptosis markers of cervical cancer and their correlation with clinicopathological parameters [J]. J Oncol, 2020, 2020:8541415. doi: 10.1155/2020/8541415.
[23] 蒋朋朋, 赵向荣, 柯小宁. Ki-67、ToPo IIα在宫颈病变中表达的比较及检出HSIL的价值分析 [J]. 医学研究杂志, 2020, 49(12): 75-78. JIANG Pengpeng, ZHAO Xiangrong, KE Xiaoning. Comparison of Ki-67, Topo IIα expressed in cervical lesions and the value analysis of HSIL detection[J]. Journal of Medical Research, 2020, 49(12): 75-78.
[24] 徐瑞云, 严晓丽, 石建芹, 等. CDC7和MCM4在子宫内膜腺癌中的表达及意义[J]. 中国妇幼保健, 2016, 31(5): 1048-1051. XU Ruiyun, YAN Xiaoli, SHI Jianqin, et a1. Expressions and significance of CDC7 and MCM4 in endometrial adenocarcinoma [J]. Maternal and Child Health Care of China, 2016, 31(5): 1048-1051.
[1] XIU Dejian, GAO Zhengwen, SONG Tingting, CUI Nan, CUI Jing, SUN Jianping. Genes associated with cervical cancer by integrated bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2022, 60(10): 99-109.
[2] LIANG Tingting, YANG Yongxia, HOU Congzhe, HUANG Taisheng, WANG Huali, ZHU Lin. Correlation among PAX1 methylation, high-grade cervical intraepithelial lesions and HR-HPV classification [J]. Journal of Shandong University (Health Sciences), 2021, 59(11): 48-52.
[3] ZHOU Huimei, YANG Jiaxin, CAO Dongyan, SHEN Keng, XIANG Yang, WU Ming, PAN Lingya, HUANG Huifang, LANG Jinghe. Fertility-sparing radical trachelectomy for young women with early stage cervical cancer: surgical and obstetrical outcomes [J]. Journal of Shandong University (Health Sciences), 2018, 56(5): 18-22.
[4] YANG Jiaxin, SHEN Keng, WANG Yao. Status and prospect of fertility preservation treatment for gynecologic malignancies [J]. Journal of Shandong University (Health Sciences), 2018, 56(5): 1-7.
[5] CHEN Ying, ZHOU Xiaoqing, QI Mei, ZHANG Weifang, LIU Juan, ZHAO Weiming. Effects of PRPS2 knockdown on the biological property of cervical cancer cells [J]. Journal of Shandong University (Health Sciences), 2018, 56(2): 9-13.
[6] WANG Yong, JIANG Chen, ZHOU Shiying, YANG Xiaomei. Effect of IQ motif-containing GTPase activating protein 3 on the proliferation and migration of cervical cancer cells [J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 103-109.
[7] LIU Jingkang, YANG Jianyong, MENG Lihua, JIANG Jie. The value of serum miR-17-92 cluster in early diagnosis of HPV-positive cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 86-90.
[8] SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.
[9] WANG Yao, CUI Guoying, WANG Fei, LI Changzhong. Expression and significance of human leucocyte antigen-E in cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 59-63.
[10] WANG Yafen, YANG Yongxia, LIU Ya, MA Demei, ZHU Lin. Use of Cervista HPV HR A9 group virus to identify patients with negative cytological but positive high-risk HPV [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 69-71.
[11] PENG Hui, HE Junmei, SU Xuelian. Expression of serum miR-375 and its clinical significance in the diagnosis and postoperative monitoring of cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 68-71.
[12] TIAN Xinli, JIAO Jun, ZHANG Teng, MA Daoxin, CUI Baoxia. Expression and significance of Th22 and Th17 cells in the peripheral blood of cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 43-47.
[13] YUAN Shoudao, WANG Fengqin, ZHANG Jiao, ZHAO Weiming, Jason J. Chen. Mass spectrometry analysis of proteins differentially expressed between adenocarcinoma and squamous carcinoma of the cervix [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 14-21.
[14] ZHANG Yanliang1, ZHUANG Zirong2, ZHU Lin1, LIANG Tingting1 . Quality of life after laparoscopic surgery for cervical cancer patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 62-66.
[15] ZHANG Lu, YAN Lei, ZHANG Hui, ZHANG Xiaohui, LI Mingjiang, ZHAO Xingbo. Expression and clinical significance of sperm-associated antigen 9 in patients with cervical cancer and cervical intraepithelial neoplasia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 67-71.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] YU Hui1,2, CHEN Shaohua1, ZHAO Jiajun2, GAO Ling3
. Effects of ethanol on glycogen content, GSK3β，
and PAMPK of L02 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(04): 75 -78 .
[2] WANG Huan-liang,SUN Bao-zhu,DU Hong-mei,ZHOU Chang-qing,ZHANG Li. Controlling the depth of propofol anesthesia using the bispectral indexand the systolic blood pressure[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 471 -474 .
[3] ZHAO Zheng-bin1， XUE Shuang-lin2， ZHANG Li-ting1， LI Jun-feng1，ZHAO Rong-rong1， ZHOU Hai-lian3， CHEN Hong1. Effects of procyanidins on the proliferation of hepatic stellate cells and production of TIMP-1 induced by leptin[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(7): 46 .
[4] MENG Jing1，LIU Xiao-li2，GUO Chun3，MA Chun-hong3. Construction of the mouse T cell immunoglobulin mucin 3(Tim-3) eukaryotic expression vector[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(11): 37 -40 .
[5] MENG Tao1, ZHANG Xin-yu2, LI Xing-hua2, YU Jin-gui1. Comparison of morphine and fentanyl on systemic inflammatory reaction and myocardial function in cardio-valve  replacement surgery under CPB[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 102 -105 .
[6] CHEN Shan-liang1， XU Li2， WANG Xue-mei2， LIU Yu-xi3， LI Quan2， ZHANG Hui2， LU Ping2， LIU Tian-qi2. Comparison of mechanical properties between glutaraldehyde tanned
donkey pericardium and bovine pericardium in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 42 .
[7] ZENG Ji-ping,WANG Li-na,WANG Li-xiang,REN Xiao-hui,ZHANG Meng-ye,XIA Wen,CUI Xing. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 467 -470 .
[8] SHU Ya,QI Feng. Jaw thrust device (JTD) on obesity patients for maintaining the airway patency during general anesthesia induction[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 1072 -1074 .
[9] SONG Yong-hong,MA Chun-hong,LV Hong-juan,ZHU Chuan-fu,NIE Xiang-min,WANG Mei,LIU Yan,ZHANG Ping. HLA allele polymorphism in northern Hans of China[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(6): 546 -553 .
[10] HUANGQing,TIAN Hui,LI Lin,LIANG Fei,LIU Xi-xian. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(6): 556 -559 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd