Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (2): 9-13.doi: 10.6040/j.issn.1671-7554.0.2017.952

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Effects of PRPS2 knockdown on the biological property of cervical cancer cells

CHEN Ying, ZHOU Xiaoqing, QI Mei, ZHANG Weifang, LIU Juan, ZHAO Weiming   

  1. Department of Pathogenic Biology, School of Basic Medical Sciences, Cheeloo College of Medical, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-28

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Abstract: Objective To evaluate the role of phosphoribosyl-pyrophosphate synthetase 2(PRPS2)on the proliferation, metastasis, cell cycle and cell apoptosis of cervical cancer cells. Methods The basal expressions of PRPS2 in cervical cancer cells(HeLa cells)and normal epithelial cells were detected with Western blotting. HeLa cells were transfected with PRPS2-specific siRNA(siPRPS2)via Lipofectamine 2000, and then divided into the knockdown group and control group. The knockdown efficiency of PRPS2 was determined with Western blotting. The cell proliferation, migration and invasion were detected with CCK-8, colony formation and Transwell assays. The effect of PRPS2 on cell cycle and apoptosis were examined with flow cytometry. Results PRPS2 was highly expressed in HeLa cells, and siPRPS2 effectively inhibited the expression of PRPS2(inhibition ratio 70%). CCK-8 and colony formation assays indicated that PRPS2 knockdown significantly inhibited the viability(P<0.001)and proliferation(P<0.01)of HeLa cells. Transwell assays showed that PRPS2 knockdown obviously reduced the number of migrated(P<0.05)and invaded(P<0.001)cells through the membrane. PRPS2 knockdown contributed to G2/M arrest(P<0.001)and apoptosis of cervical cancer cells(P=0.012). Conclusion PRPS2 can promote the proliferation, metastasis and cell cycle of cervical cancer cells.

Key words: Cervical cancer, Phosphoribosyl-pyrophosphate synthetase 2, Proliferation, Migration, Invasion

CLC Number: 

  • R737
[1] Hove-Jensen B, Andersen KR, Kilstrup M, et al. Phosphoribosyl diphosphate(PRPP): biosynthesis, enzymology, utilization, and metabolic significance[J]. MicrobiolMolBiol Rev, 2016, 81(1). pii: e00040-16. doi: 10.1128/MMBR.00040-16.
[2] de Brouwer AP, van Bokhoven H, Nabuurs SB, et al. PRPS1 mutations: four distinct syndromes and potential treatment[J]. Am J Hum Genet, 2010, 86(4): 506-518.
[3] Iizasa T. Increased activity of PRPP synthetase[J]. Nihon Rinsho, 2008, 66(4): 694-698.
[4] Cunningham JT, Moreno MV, Lodi A, et al. Protein and nucleotide biosynthesis are coupled by a single rate-limiting enzyme, PRPS2, to drive cancer[J]. Cell, 2014, 157(5): 1088-1103.
[5] Schiffman M, Doorbar J, Wentzensen N, et al. Carcinogenic human papillomavirus infection[J]. Nature reviews Disease primers, 2016, 2(1): 16086.
[6] Münger K, Baldwin A, Edwards KM, et al. Mechanisms of human papillomavirus induced oncogenesis[J]. J Virol, 2004, 78(21): 11451-11460.
[7] Zhang X, Chen H, Wang X, et al. Expression and transcriptional profiling of the LKB1 tumor suppressor in cervical cancer cells[J]. GynecolOncol, 2014, 134(2): 372-378.
[8] Cairns RA, Harris IS, Mak TW. Regulation of cancer cell metabolism[J]. Nat Rev Cancer, 2011, 11(2): 85-95.
[9] Mayers JR, Vander Heiden MG. Nature and nurture: what determines tumor metabolic phenotypes?[J]. Cancer Res, 2017, 77(12): 3131-3134.
[10] Martinez-Outschoorn UE, Peiris-Pages M, Pestell RG, et al. Cancer metabolism: A therapeutic perspective[J]. Nat Rev Clin Oncol, 2016, 14(2): 11-31.
[11] Xue C, Yu DM, Gherardi S, et al. MYCN promotes neuroblastoma malignancy by establishing aregulatory circuit with transcription factor AP4[J]. Oncotarget, 2016, 7(34): 54937-54951.
[12] Lei B, Wan B, Peng J, et al. PRPS2 expression correlates with sertoli-cell only syndrome and inhibits the apoptosis of TM4 sertoli cells[J]. J Urol, 2015, 194(5): 1491-1497.
[13] Mannava S, Grachtchouk V, Wheeler LJ, et al. Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells[J]. Cell Cycle, 2008, 7(15): 2392-2400.
[14] Wang J, Wang J, Zhang Y, et al. Proteomic analysis on infantile spasm and prenatal stress[J]. Epilepsy Res, 2014, 108(7): 1174-1183.
[15] zur Hausen H. Papillomaviruses and cancer: from basic studies to clinical application[J]. Nat Rev Cancer, 2002, 2(5): 342-350.
[16] zur Hausen H. Papillomaviruses in the causation of human cancers -a brief historical account[J]. Virology, 2009, 384(2): 260-265.
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