Abstract: Objective To explore the effect of miR-584 expression on the invasion and migration of glioblastoma U251 cells in vitro. Methods Chemosynthetic miR-584-3p was transfected into glioblastoma cells under the induction of Lipofectamine 2000. The cells were divided into control group(no transfection), mimics NC group(transfected with negative control sequence of miR-584-3p mimics), miR-584-3p mimics group(transfected with miR-584-3p mimics), inhibitor NC group(transfected with negative control sequence of miR-584-3p inhibitor), miR-584-3p inhibitor group(transfected with miR-584-3p inhibitor).The expression of miR-584-3p was detected by Real-time PCR. Cell proliferation was detected by CCK-8 proliferation assay. The invasive and migratory effects of glioblastoma cell were measured by Wound healing and Transwell assays. Results Compared with mimics NC group and control group, the expression of miR-584-3p in miR-584-3p mimics group was upregulated by 100 times(P< 0.05). Compared with mimics NC group and control group, the cell proliferation ability of miR-584-3p mimics group showed no significant change(P >0.05), but the cellular migratory and invasive activity of miR-584-3p mimics group were down-regulated significantly(P <0.05). Compared with inhibitor NC group and control group, the expression of miR-584-3p in miR-584-3p inhibitor group was down-regulated by 20 times(P <0.05). Compared with inhibitor NC group and control group, the cell proliferation ability of miR-584-3p inhibitor group showed no significant change(P >0.05), the cellular migration and invasive activity in miR-584-3p inhibitor group were up-regulated significantly(P <0.05). Conclusion The migration and invasion of glioblastoma cells can be inhibited by the over-expression of miR-584-3p, while the transfection of miR-584-3p inhibitor can enhance the migration and invasion of glioblastoma cells.

Key words: MiR-584, Glioblastoma, Invasion, Migration