Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (8): 74-80.doi: 10.6040/j.issn.1671-7554.0.2020.0601

• Special Topic on Brain Science and Brain Like Intelligence • Previous Articles     Next Articles

Overview of glioblastoma models and development and application of brain organoids

Jian WANG1,2,3,*(),Wenjing ZHOU1,2,Zhiyi XUE1,2,Xiaofei LIU1,2   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, Shandong, China
    2. Shandong Key Laboratory of Brain Function Remodeling, Jinan 250012, Shandong, China
    3. Department of Biomedicine, University of Bergen, Bergen 5009, Vestland, Norway
  • Received:2020-04-15 Online:2020-08-01 Published:2020-08-07
  • Contact: Jian WANG E-mail:jian.wang@sdu.edu.cn

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Abstract:

Glioblastoma (GBM) is the most common primary brain tumor with high morbidity and high mortality. The primary goal of researches on GBM is to establish models to recapitulate the tumor invasive phenotype. In this review, we have systematically summarized the in vitro and in vivo models in GBM study by discussing the establishment and applications as well as their limitations. We acknowledge the development of various model systems in recent years which has substantially improved GBM research activities. However, largely due to difficulties of recapitulating the complicated microenvironment in GBM, it remains challenging to explore the tumor developmental mechanism and candidate drug efficacy in the treatment. Our team has extensively studied GBM for many years and established an ex-vivo model using rat brain organoids. Extensive data show that the model can highly mimic the process of brain development. Together with GBM patient-derived tumor spheroids and brain organoids, we have established co-culture model systems in order to in real time study the invasive GBM, which may fundamentally help us to understand the tumor development. Moreover, using the system, we are able to sort the invasive tumor cells from non-invasive tumor cells and analyze single cell RNA sequences to identify key molecules, which will be possible in turn to offer us new therapeutic candidates potentially targeting GBM.

Key words: Glioblastoma, Ex-vivo model, Brain organoid, Tumor spheroid, Co-culture

CLC Number: 

  • R739.4

Fig.1

A schematic overview of the establishment of rat brain organoids and co-culture system by confronting rat brain organoids with 3D patient-derived GBM spheroids."

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