JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 1-6.doi: 10.6040/j.issn.1671-7554.0.2015.1162

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Combination of bone marrow mesenchymal stem cells, platelet gel and extrocorporeal shock wave on bone regeneration

SONG Ke, LIU Huan, WU Wenliang, LIU Haichun, LI Shangzhi, CHEN Yunzhen   

  1. Department of Orthopedics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-25 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To evaluate whether platelet gel(PG)and extracorporeal shock wave(ESW)could enhance the effect of bone marrow mesenchymal stem cell(BMSCs)in the treatment of nonunion and to select the best therapy on bone regeneration. Methods A total of 125 atrophic nonunion models of rats were established and randomly divided into 5 groups with 25 animals in each group: nonunion group, BMSCs group, BMSCs+PG group, BMSCs+ESW group, and BMSCs+PG+ESW group. Bone regeneration was evaluated via X-ray and micro-CT 4 and 8 weeks after treatment. All rats were sacrificed for histological analysis in week 8. Results X-ray images showed that the ratio of bone union in BMSCs+PG+ESW group was the highest(P<0.05). Micro-CT showed that BMSCs+PG+ESW group contained significantly larger bone volume fraction, thicker and higher number of bone trabecular, and smaller ratio of bone surface / bone volume compared with the other 4 groups(P<0.05). Histomorphometry showed that the BMSCs+PG+ESW group had the largest number of osteoblasts, vascular buds and bone areas fraction among all groups(P<0.05). Conclusion PG and ESW can enhance the effect of BMSCs on bone regeneration. Combination of BMSCs, PG and ESW can produce better results than the combination of two items and monotherapy.

Key words: Nonunion, Extrocorporeal shock wave, Mesenchymal stem cell, Micro-CT, Platelet gel

CLC Number: 

  • R683.4
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