JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 16-20.doi: 10.6040/j.issn.1671-7554.0.2015.1029

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Effect of umbilical cord mesenchymal stem cells on microglial polarization and proliferation

WANG Na, CHEN Naiyao   

  1. Department of Hematology, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei, China
  • Received:2015-10-29 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To observe the effect of umbilical cord mesenchymal stem cells(hUC-MSCs)on the microglia proliferation and activation in normal or inflammatory conditions, in order to explore their immunomodulation effect on the central nervous system. Methods After BV2 cells were co-cultured with hUC-MSCs-CM and/or lipopolysaccharide(LPS)for 24 or 48 h, the proliferation was analyzed by MTT colorimetric method, TNF-α and IL-6 in the supernatant were measured by ELISA kit, Arginase 1(Arg1)protein and mRNA levels were examined by Western blotting, TNF-α and IL-6 mRNA expression level were tested by real-time PCR. Results (1) After long-time LPS stimulation, BV2 cells obviously proliferated, while hUC-MSCs-CM inhibited the proliferation. (2) BV2 cultured with LPS expressed high levels of TNF-α and IL-6, while in both hUC-MSCs-CM and LPS conditions, the TNF-α and IL-6 levels were lower than in LPS stimulation. (3) The level of Arg1 was increased in BV2 cells co-cultured with hUC-MSCs-CM for 48 h. Conclusions Human UC-MSCs inhibit microglia proliferation caused by inflammatory stimulation. Human UC-MSCs induce microglial cells to M2 polarization and reduce the expression of proinflammatory factors via paracrine mechanism, and this may be the mechanism for its neuroprotective effect.

Key words: Neuroprotection, Immunomodulation, Microglia, Umbilical cord mesenchymal stem cells

CLC Number: 

  • R392.1
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