JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 1-6.doi: 10.6040/j.issn.1671-7554.0.2015.1162

    Next Articles

Combination of bone marrow mesenchymal stem cells, platelet gel and extrocorporeal shock wave on bone regeneration

SONG Ke, LIU Huan, WU Wenliang, LIU Haichun, LI Shangzhi, CHEN Yunzhen   

  1. Department of Orthopedics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-25 Online:2016-06-20 Published:2016-06-20

Abstract: Objective To evaluate whether platelet gel(PG)and extracorporeal shock wave(ESW)could enhance the effect of bone marrow mesenchymal stem cell(BMSCs)in the treatment of nonunion and to select the best therapy on bone regeneration. Methods A total of 125 atrophic nonunion models of rats were established and randomly divided into 5 groups with 25 animals in each group: nonunion group, BMSCs group, BMSCs+PG group, BMSCs+ESW group, and BMSCs+PG+ESW group. Bone regeneration was evaluated via X-ray and micro-CT 4 and 8 weeks after treatment. All rats were sacrificed for histological analysis in week 8. Results X-ray images showed that the ratio of bone union in BMSCs+PG+ESW group was the highest(P<0.05). Micro-CT showed that BMSCs+PG+ESW group contained significantly larger bone volume fraction, thicker and higher number of bone trabecular, and smaller ratio of bone surface / bone volume compared with the other 4 groups(P<0.05). Histomorphometry showed that the BMSCs+PG+ESW group had the largest number of osteoblasts, vascular buds and bone areas fraction among all groups(P<0.05). Conclusion PG and ESW can enhance the effect of BMSCs on bone regeneration. Combination of BMSCs, PG and ESW can produce better results than the combination of two items and monotherapy.

Key words: Nonunion, Extrocorporeal shock wave, Mesenchymal stem cell, Micro-CT, Platelet gel

CLC Number: 

  • R683.4
[1] Arinzeh TL, Peter SJ, Archambault MP, et al. Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect[J]. J Bone Joint Surg Am, 2003, 85(10): 1927-1935.
[2] Kitoh H, Kitakoji T, Tsuchiya H, et al. Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis-a preliminary result of three cases[J]. Bone, 2004, 35(4): 892-898.
[3] Gandhi A, Bibbo C, Pinzur M, et al. The role of platelet-rich plasma in foot and ankle surgery[J]. Foot Ankle Clin, 2005, 10(4): 621-637.
[4] Kawasumi M, Kitoh H, Siwicka KA, et al. The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone[J]. J of Bone Joint Surg Br, 2008, 90(7): 966-972.
[5] Furia JP, Rompe JD, Cacchio A, et al. Shock wave therapy as a treatment of nonunions, avascular necrosis, and delayed healing of stress fractures[J]. Foot and Ankle Clinics, 2010, 15(4): 651-662.
[6] Borrelli J, Prickett WD, Ricci WM. Treatment of nonunions and osseous defects with bone graft and calcium sulfate[J]. Clin Orthop Relat Res, 2003, 14(411): 245-254.
[7] Rodriguez-Merchan EC, Forriol F. Nonunion: general principles and experimental data[J]. Clin Orthop Relat Res, 2004, 20(419): 4-12.
[8] Goel A, Sangwan SS, Siwach RC, et al. Percutaneous bone marrow grafting for the treatment of tibial non-union[J]. Injury, 2005, 36(1): 203-206.
[9] Ma HL, Chen TH, Hung SC. Development of a new method in promoting fracture healing: multiple cryopreserved bone marrow injections using a rabbit model[J]. Arch Orthop Trauma Surg, 2004, 124(7): 448-454.
[10] 刘亚云, 杨保华, 陈光连. 经皮自体移植浓缩骨髓干细胞治疗骨不连的实验研究[J]. 江西医药, 2013, 15(11): 968-970.
[11] 庞伟峰, 李会军, 李东利,等. 富血小板血浆在骨折不愈合中的应用[J]. 中国矫形外科杂志, 2015, 20(16): 1535-1536.
[12] Marx RE, Carlson ER, Eichstaedt RM, et al. Platelet-rich plasma: growth factor enhancement for bone grafts[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1998, 85(6): 638-646.
[13] Freymiller EG, Aghaloo TL. Platelet-rich plasma:ready or not?[J]. J Oral Maxillofac Surg, 2004, 62(4): 484-488.
[14] Marx R. Platelet-rich plasma: evidence to support its use-reply[J]. J Oral Maxillofac Surg, 2004, 62(8): 1046-1048.
[15] Weibrich G, Hansen T, Kleis W, et al. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration[J]. Bone, 2004, 34(4): 665-671.
[16] Anitua E, Andia I, Ardanza B, et al. Autologous platelets as a source of proteins for healing and tissue regeneration[J]. Thromb Haemost, 2004, 91(1): 4-15.
[17] Eckardt H, Bundgaard KG, Christensen KS, et al. Effects of locally applied vascular endothelial growth factor(VEGF)and VEGF-inhibitor to the rabbit tibia during distraction osteogenesis[J]. J Orthop Res, 2003, 21(2): 335-340.
[18] Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis[J]. Biomaterials, 2005, 26(27): 5474-5491.
[19] Chen YJ, Wurtz T, Wang CJ, et al. Recruitment of mesenchymal stem cells and expression of TGF-beta 1 and VEGF in the early stage of shock wave-promoted bone regeneration of segmental defect in rats[J]. J Orthop Res, 2004, 22(3): 526-534.
[20] Hausdorf J, Sievers B, Schmitt-Sody M, et al. Stimulation of bone growth factor synthesis in human osteoblasts and fibroblasts after extracorporeal shock wave application[J]. Arch Orthop Trauma Surg, 2011, 131(3): 303-309.
[1] WANG Kaimin, TAN Juanjuan, YAN Zhiqiang, LI Zhiqiang. Effects of Sirtuin on hypoxia-induced proliferation of human umbilical cord-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 23-30.
[2] LI Jingyuan, SONG Ling, LIN Song, SONG Hui. Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 31-37.
[3] LIU Ning, L(¨overU)Xin, LI Dong, HUNAG Zhiwei, LIU Yi, ZHANG Leling. Construction and identification of lentiviral vector for angiopoietin-1 gene and its expression in human umbilical cord mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 1-7.
[4] WANG Na, CHEN Naiyao. Effect of umbilical cord mesenchymal stem cells on microglial polarization and proliferation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 16-20.
[5] LI Shangzhi, LIU Haichun, WU Wenliang, CAO Cong, CHEN Yunzhen. Curative effect of mechanical stress in the treatment of nonunion [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 22-26.
[6] FU Haiyan, HU Zhansheng, DU Hongyang, LI Chao, BAO Cuifen. Effect of rehmannia glutinosa polysaccharide on the differentiation and proliferation of over-expressed Notch1(NICD) gene in rat bone marrow mesenchymal stem cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 34-40.
[7] LIU Juan, HAN Guoqing, LIU Hui, QIN Chengyong. Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 1-5.
[8] LIU Jinwei1, LIU Haichun1, WU Wenliang1, CHEN Liang2, LI Shangzhi1, CAO Cong1, CHEN Yunzhen1. A femoral osteoporotic nonunion model established with an adjustable external fixator in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 33-36.
[9] GE Jun-ke, ZHAO Sheng-tian. Protective effect of bone marrow mesenchymal stem cells and CD133+ renal cells on acute renal injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(9): 55-59.
[10] HU Su1,2, PANG Shu-guang2, CUI Ying2, YU Chun-xiao1, ZHAO Jia-jun1, GUAN Qing-bo1. Osteogenic potential of bone marrow mesenchymal stem cells from streptozotocininduced diabetic rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 7-12.
[11] CHEN Yan1, LI Dong2, ZHANG Zhe1, SHI Qing2, WANG Da-kun2, LIU Huan2, WANG Guo-yun1. Effects of human umbilical cord mesenchymal stem cells on nerve fibers in the lesions of rat endometriosis  model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 48-51.
[12] DU Hong-yang1, LI Dong-ning1, FU Hai-yan2, BAO Cui-fen2, QIN Shu-jian1. Effect of rehmannia glutinosa polysaccharide on Notch signal pathway in rat bone marrow mesenchymal stem cells during differentiation into neuron-like cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(12): 1-6.
[13] SHANG Xiao1, CUI Yuan-xiao1, LIU Xiao-yu1, FENG Xiao-ya2. Experimental study of human umbilical cord mesenchymal stem cells combined with minimal hematoma aspiration in the therapy of rats with intracerebral hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 10-14.
[14] CHEN Nai-yao1, SHEN Na2, HE Jing-an3, ZHENG Li-kun4. Changes of BDNF after treatment of traumatic brain injury with human umbilical cord blood mesenchymal stem cells in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(1): 22-.
[15] LIU Hua1,2, CHEN Ya-na3, ZHENG Yan1, CHEN Li4, LI Cai-xin4, ZHUANG Xiu-ping4, WANG Yun-shan1,2. Effects of human umbilical cord mesenchymal stem cells on the growth of Hela cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(06): 29-33.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!