JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 16-21.doi: 10.6040/j.issn.1671-7554.0.2015.1096

Previous Articles     Next Articles

CXCL16 gene silencing alleviates injury of mouse podocytes treated with the oxidized low-density lipoprotein

WANG Xue1, LI Qian1, WANG Li1, SUN Shuzhen1, MA Aihua2   

  1. 1. Department of Paediatric Nephrology &
    Rheumatism and Immunology, 2. Department of Paediatric Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-11-10 Online:2016-06-20 Published:2016-06-20

PDF (PC)

225

Abstract: Objective To investigate the role of chemokine ligand 16(CXCL16)gene silencing on alleviating injury of podocytes treated with oxidized low-density lipoprotein(ox-LDL). Methods The mouse podocytes were transfected with CXCL16 gene short hairpin RNA(shRNA)lentivirus vector and negative control lentivirus vector. The protein and mRNA expressions of CXCL16 were determined with Western blotting and Real-time PCR. The vitality of podocytes was detected with CCK8 assay. The mouse podocytes(cell line)were divided into 4 groups: negative control group(NC group), ox-LDL group, CXCL16-shRNA group, and ox-LDL+CXCL16-shRNA group. The total cholesterol was detected with cholorimetric detection to measure intracelluar lipid accumulations. The expressions of CXCL16 and integrin-β1 were detected with Western blotting. The expression of ROS was detected with ROS Deep Red Dye. Results The inhibition rate of CXCL16 mRNA was about 70%, and that of CXCL16 protein was about 60%, compared to the negative control(both P=0.008). CCK8 revealed no significant difference in the vitality of podocytes between NC group and CXCL16-shRNA group(P=0.712). Compared to the ox-LDL group, in the ox-LDL+CXCL16-shRNA group, the intracelluar lipid accumulations were reduced(P<0.001), integrin-β1 was increased(P=0.004), 山 东 大 学 学 报 (医 学 版)54卷6期 -王雪,等.CXCL16基因沉默减轻ox-LDL对小鼠足细胞损伤 \=-and ROS was reduced(P<0.001). Conclusion CXCL16 gene silencing is likely to play an important role for podocytic protection after the treatment of ox-LDL by reducing the synthesis of ROS and elevating the expression of integrin-β1.

Key words: Chemokine ligand 16, Reactive oxygen species, Integrin-β1, Podocyte, Oxidized low-density lipoprotein, Short hairpin RNA

CLC Number: 

  • R726.9
[1] Diez-Sampedro A, Lenz O, Fornoni A, et al. Podocytopathy in diabetes: a metabolic and endocrine disorder[J]. Am J Kidney Dis, 2011, 58(4): 637-646.
[2] Gutwein P, Abdel-Bakky MS, Schramme A, et al. CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein[J]. Am J Pathol, 2009, 174(6): 2061-2072.
[3] 王聪, 孙书珍, 甄军晖, 等. CXCL16在阿霉素肾病小鼠中的作用及辛汀对其影响的作用[J]. 山东大学学报(医学版), 2015, 53(9): 24-29. WANG Cong, SUN Shuzhen, ZHEN Junhui, et al. Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin[J]. Journal of Shandong University(Health Sciences), 2015, 53(9): 24-29.
[4] Wang L, Sun S, Zhou A, et al. Ox-LDL-induced lipid accumulation in glomerular podocytes: role of IFN-γ, CXCL16, and ADAM10[J]. Cell Biochem Biophys, 2014, 70(1): 529-538.
[5] 许艺怀, 孙书珍, 甄军晖, 等. CXCL16和oxLDL在阿霉素肾病小鼠中的变化及意义[J]. 山东大学学报(医学版), 2014, 52(5): 68-72. XU Yihuai, SUN Shuzhen, ZHEN Junhui, et al. Role of CXCL16 and ox-LDL in mice with adriamycin induced nephropathy[J]. Journal of Shandong University(Health Sciences), 2014, 52(5): 68-72.
[6] Norlander AE, Saleh MA, Madhur MS, et al. CXCL16: a chemokine causing chronic kidney disease[J]. HHS Author Manuscriopts, 2013, 62(6): 1008-1010.
[7] Gutwein P, Abdel-Bakky MS, Doberstein K, et al. CXCL16 and ox-LDL are induced in the onset of diabetic nephropathy[J]. J Cell Mol Med, 2009, 13(9b): 3809-3825.
[8] Daehn I, Casalena G, Zhang T, et al. Endothelial mitochondrial oxidative stress determines podocyte depletion in segmental glomerulosclerosis[J]. J Clin Invest, 2014, 124(4): 1608-1621.
[9] Herman-Edelstein M, Scherzer P, Tobar A, et al. Altered renal lipid metabolism and renal lipid accumulation in human diabetic nephropathy[J]. J Lipid Res, 2014, 55(3): 561-572.
[10] Dai R, Lin Y, Liu H, et al. A vital role for Angptl3 in the PAN-induced podocyte loss by affecting detachment and apoptosis in vitro[J]. BMC Nephrol, 2015, 16: 38, doi:10.1186/s12882-015-0034-4.
[11] Pozzi A, Jarad G, Moeckel GW, et al. β1 integrin expression by podocytes is required to maintain glomerular structural integrity[J]. Dev Biol, 2008, 316(2): 288-301.
[12] Müller-Deile J, Schiffer M. The podocyte power-plant disaster and its contribution to glomerulopathy[J]. Front Endocrinol, 2014, 5: 209, doi: 10.3389/fendo.2014.00209.
[13] Zorov DB, Juhaszova M, Sollott SJ, et al. Mitochondrial reactive oxygen species(ROS)and ROS-induced ROS release[J]. Physiol Rev, 2014, 94(3): 909-950.
[1] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24-31.
[2] ZHAO Zuohui, LI Cuiling, WANG Daoguang, WANG Fengqin, QU Hongyi, DING Sentai, GONG Jing, LÜ Jiaju, YANG Jinghua. Effect of MnSOD acetylation on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 31-35.
[3] LI Shuai, WANG Yalin, SUN Zhongwen, ZHU Meijia. Expression changes and mechanism of Nod-like receptor protein 3 inflammasome in the cerebral microvascular endothelial cells in type 2 diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 6-11.
[4] WANG Cong, SUN Shuzhen, ZHEN Junhui, LI Qian, XU Yihuai. Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 24-29.
[5] LIU Hui, CHEN Tongshuai, LI Na, WANG Shujian, LI Jingyuan, BU Peili. Role of Sirt3 on the senescence of human umbilical vein endothelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 41-45.
[6] YANG Lu, ZHOU Yingze, NI Ming, FAN Xiushuang, MAN Jianmei, GUO Juntang. Beta-synuclein promotes the expression of vesicular monoamine transporter-2 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 61-64.
[7] QIU Zongjian, YANG Juan, SONG Qiang. Role of decitabine in the mitochondria biogenesis of synchronized G0/G1 phase MDS-L cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 5-9.
[8] SUN Gao-ying1, WANG Xiao-yuan2, ZHANG Xiao-jin1, HAO Xiu-yu1， GUO Yuan-fang1, WANG Xiao1, BI Wen-xiang1. Effects of N-acetylcysteine  on resistance to paclitaxel in cervical carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 42-47.
[9] CHANG Xiang-di1, WANG Jie1, LI Bing1, ZHEN Jun-hui2, JIANG Hong3, HU Zhao1. Protective effect of FK506 on glomerular podocyte in
early diabetic nephropathy rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 20-25.
[10] JIANG Chong-hui1, AI Xiao-hong2, YANG Xiao-hong3, LUO Qiong3, CAO Jian-guo3. Generation of reactive oxygen species in apoptosis of human gastric cancer
SGC-7901 cells induced by 5,7-Dihydroxy-8-nitrochrysin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 34-37.
[11] DONG Chuan-fang1, LIU Xue-ping1, WANG Mei-xia1, XU Song1, YIN Qin-qing1, SUN Zhi-jian1, YOU Li2. Effect of advanced glycation end products on oxidative stress andapoptosis of SH-SY5Y cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 9-.
[12] SUN Rong-guo1, XU Long-jin2, HU Hong-yan1, CAI Xiao-jun1, WANG Meng1, Lv Yi-jie1 . Effect of polyinosinic acid on expression of lectin-like oxidized low-density lipoprotein receptor-1 and its  antiatherogenic role in atherosclerotic rabbits [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 78-82.
[13] ZHU Wei1,2， PAN Fang1， JIANG Hong1， YU Hong-luan1， SUN Lin1， WANG Li1. Effects of ketamine on cerebrovascular pathology and NADPH oxidase activation in cynomolgus monkeys [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 47-51.
[14] YIN Hai-qin1, WEI Yu-ping2, WANG Xiao-yuan3， PENG Nan1, BI Wen-xiang2, KONG Feng2, ZHANG Pei-hai1. Comparison of antioxidant abilities of cervical squamous  carcinoma and adenocarcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 55-.
[15] YIN Hai-qin1, WEI Yu-ping2, WANG Xiao-yuan3， PENG Nan1, BI Wen-xiang2, KONG Feng2, ZHANG Pei-hai1. Comparison of antioxidant abilities of cervical squamous  carcinoma and adenocarcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 55-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd