阿尔茨海默病细胞模型中lncRNA RP11-543N12.1对CDH13表达的调控作用

doi:10.6040/j.issn.1671-7554.0.2016.102

Abstract

Abstract: Objective To explore the regulatory function of long noncoding RNA(lncRNA)RP11-543N12.1 on the expression of CDH13 in Alzheimers disease(AD)cell model. Methods Firstly, differently expressed lncRNAs and mRNAs were screened through microarray, and upregulated expressions of both RP11-543N12.1 and CDH13 were verified by real-time PCR. Secondly, SH-SY5Y cells were transfected with RP11-543N12.1-siRNA, and then Aβ_25-35 was added to the transfected cells. After 48 h, the survival rate was detected with MTT; RNA expressions of RP11-543N12.1 and CDH13 were detected with real-time PCR; protein expression of CDH13 was determined with Western blotting; morphological changes were observed with Hoechst33258 staining; cellular apoptosis rate was detected with flow cytometry. Results Compared with control SH-SY5Y cells, cells treated with Aβ_25-35 showed inhibited proliferation(P<0.05), upregulated RNA and protein expressions of CDH13(both P<0.01), and increased cellular apoptosis rate(P<0.01). Apoptotic nuclei were also observed. In cells transfected with RP11-543N12.1-siRNA and treated with Aβ_25-35, cellular survival rate, RNA and protein expressions, and cellular apoptosis rate were all close to normal levels. Nuclei apoptosis disappeared. Conclusion LncRNA RP11-543N12.1 can regulate expression of CDH13, and knock-down of RP11-543N12.1 can downregulate the expression of CDH13.

Key words: CDH13, Expression regulation, SH-SY5Y cell, LncRNA RP11-543N12.1, Alzheimers disease

CLC Number:

R574

LI Mengmeng, WANG Miaomiao, DIAO Xueqin, TIAN Keli, XU Xia, REN Guijie. Regulatory effect of lncRNA RP11-543N12.1 on the expression of CDH13 in Alzheimers disease cell model[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 12-18.

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Regulatory effect of lncRNA RP11-543N12.1 on the expression of CDH13 in Alzheimers disease cell model

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