JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 19-24.doi: 10.6040/j.issn.1671-7554.0.2016.187

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FOXM1 regulates transcription of SYK in breast cancer cell line MCF-7

MENG Fanchao1, FENG Lu2, SHAO Guangrui1   

  1. 1. Department of Imaging;
    2. Department of Clinical Laboratory, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2016-02-25 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To explore the underlying mechanism of Forkhead box M1(FOXM1)regulating spleen tyrosine kinase(SYK)expression in breast cancer cell line MCF-7. Methods PCR and Western blotting assay were used to analyze the mRNA and protein levels of SYK in MCF-7, T47D, SKBR3 and MDA-MB-231 cells. The methylation status of SYK promoter CpG island was detected. FOXM1 was overexpressed in MCF-7 cells, and then mRNA and protein levels of SYK and FOXM1 were detected. Chromatin immunoprecipitation(ChIP)assay and luciferase reporter assay were adopted to examine whether FOXM1 directly regulated the promoter activity of SYK. Results MCF-7 cells expressed endogenous SYK while T47D, SK-BR3 and MDA-MB-231 cells did not. The rate of DNA methylation of SYK in MCF-7 cells was lower than that in control cells(P<0.05). Overexpression of FOXM1 decreased SYK expression in MCF-7 cells and silence of FOXM1 in SK-BR3 cells increased SYK expression. ChIP assay indicated that FOXM1 directly regulated the transcriptional level of SYK gene. Knockdown of FOXM1 increased the basal SYK promoter activity and overexpression of FOXM1 decreased luciferase activity of SYK in MCF-7 cells. Conclusion SYK is a target gene of FOXM1 in MCF-7, while FOXM1 is involved in the genesis and development of breast cancer by directly regulating SYK.

Key words: MCF-7 cells, Forkhead box protein M1, Transcriptional regulation, Breast cancer, Spleen tyrosine kinase

CLC Number: 

  • R34
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