JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 60-64.doi: 10.6040/j.issn.1671-7554.0.2014.303

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Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro

ZHUANG Yong1, LI Dong2, FU Jinqiu1, SHI Qing2, JU Xiuli1   

  1. 1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-05-09 Published:2014-11-10

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Abstract: Objective To induce and culture dendritic cells (DC) derived from normal children and childhood B lineage acute lymphoblastic leukemia (B-ALL) patients, and compare their biological features in vitro. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from 10 B-ALL patients (ALL group) and 10 healthy donors (control group). The isolated PBMCs were co-cultured with rhGM-CSF(20 ng/mL), rh IL-4(10 ng/mL) and TNF-α(10 ng/mL) for 8 days. The morphological features were observed by inverted microscope. CD11c, CD80, CD83, CDD86 expressions were assayed by flow cytometry. The concentration of IL-12 was measured by ELISA. The antigen presenting function of the cells were tested by mixed lymphocyte reaction(MLR). Electrochemical measurement was used for the detection of glucose metabolism. Results The typical DC morphological features were not observed from the cells in ALL group. The cells in ALL group expressed lower levels of CD11c, CD83, CD80 and CD86 than the cells in control group(P<0.05). The cells in ALL group secreted lower level of IL-12 than control group(P<0.05). The cells in ALL group displayed weaker activity of stimulating T lymphocyte proliferation than control group in MLR test. Glucose concentration in culture medium of ALL group was higher than that of control group(P<0.05). Conclusion DC derived from B-ALL patients displays a less mature phenotype with compromised functions. Low glucose metabolism ability might be a possible reason for the DC defects in ALL.

Key words: Glucose metabolisom, Leukemia, acute, Lymphoblastic, Dendritic cells

CLC Number: 

  • R725.5
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