JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (4): 76-81.doi: 10.6040/j.issn.1671-7554.0.2016.782

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Expression and role of Helios gene in regulatory T cells of pediatric precursor B-cell acute lymphoblastic leukemia

LI Xue1,2, LI Dong2, SHI Qing2, ZHOU Panpan2, JU Xiuli1,2   

  1. 1. Shenzhen Research Institute, Shandong University, Shenzhen 518057, Guangdong, China;
    2. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-07-05 Online:2017-04-10 Published:2017-04-10

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Abstract: Objective To detect the proportion of human CD4+CD25+FoxP3+ regulatory T cells(Treg)in patients with pre-B acute lymphoblastic leukemia(ALL)and to explore the role of transcriptional factor Helios in pre-B ALL Treg cells. Methods A total of 10 primary ALL cases(ALL group)and 6 cases of osteoarthropathy free from tumors, hematological diseases and immunological diseases(control group)were involved. Peripheral blood samples were collected from all cases. The proportion of Treg cells was tested with flow cytometry, and the expressions of Helios, FoxP3 and related transcription inhibitors in CD4+CD25+ Treg cells of pre-B ALL samples were tested with real-time quantitative polymerase chain reaction(qRT-PCR). The lentiviral vectors expressing Helios mRNA and Helios-siRNA were constructed to regulate Helios expression in vitro to assay the role of Helios. Results The percentage of Treg cells in the ALL group and control group was(6.16±0.79)% and(2.62±0.34)%, respectively, with significant statistical difference(P=0.005). The expressions of Helios, FoxP3 and in TGF-β1 mRNA were upregulated in Treg cells. The abnormal expression of Helios was positively correlated to the immunosupression function of Treg cells. 山 东 大 学 学 报 (医 学 版)55卷4期 -李雪,等.Helios在儿童急性淋巴细胞性白血病调节性T细胞中的表达及功能 \=- Conclusion The immunosuppressive function of Treg cells can affect the immunity of pediatric pre-B ALL by regulating the expression of Helios gene. Helios gene plays an important role in the function of ALL Treg cells. Helios may serve as a target gene in the immunotherapy of Treg-dependent cancer.

Key words: Helios gene, TGF-β1, Acute lymphoblastic leukemia, Regulatory T cells

CLC Number: 

  • R725.5
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