JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 41-44.doi: 10.6040/j.issn.1671-7554.0.2014.538

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Preliminary study in the protective role of p120 catenin in inflammatory lung injury

SUI Xiangyu, ZHANG Xiangchun, ZHANG Guangyong, HU Sanyuan, WANG Yanlei, DAI Yong   

  1. Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-08-18 Revised:2014-11-12 Published:2014-12-10

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Abstract: Objective To investigate the role of p120 catenin in inflammatory cytokine tumor necrosis factor-α (TNF-α)-induced lung microvascular endothelial cells activation, and explore the underlying mechanism. Methods p120 expression level in endothelial cells was downregulated with specific p120 small interference RNA (siRNA). Difference of TNF-α-induced nuclear factor-κB (NF-κB) activation, intracellular adhesion molecular-1(ICAM-1) expression, and neutrophil transendothelial migration were detected. Mechanism of p120's regulatory role in inflammatory lung injury with specific NF-κB inhibitor was investigated. Results TNF-α reduced p120 level in lung microvascular endothelial cells. p120 downregulation in endothelial cells significantly aggravated TNF-α-induced NF-κB activation, ICAM-1 expression, and neutrophil transendothelial migration. These effects were remarkably suppressed by NF-κB inhibitor. Conclusion p120 plays a critical protective role in TNF-α-induced lung microvascular endothelial cells activation and subsequent neutrophil transendothelial migration through the inhibition of NF-κB activation.

Key words: Endothelial cells, Lung injury, Catenin, p120, Inflammatory cytokine

CLC Number: 

  • R563.1
[1] Hu G. p120-Catenin: a novel regulator of innate immunity and inflammation[J]. Crit Rev Immunol, 2012, 32(2): 127-138.
[2] Kourtidis A, Ngok S P, Anastasiadis P Z. p120 catenin: an essential regulator of cadherin stability, adhesion-induced signaling, and cancer progression[J]. Prog Mol Biol Transl Sci, 2013, 116:409-432.
[3] Yang Z, Sun D, Yan Z, et al. Differential role for p120-catenin in regulation of TLR4 signaling in macrophages[J]. J Immunol, 2014, 193(4): 1931-1941.
[4] Zhou Z, Connell M C, MacEwan D J. TNFR1-induced NF-kappaB, but not ERK, p38MAPK or JNK activation, mediates TNF-induced ICAM-1 and VCAM-1 expression on endothelial cells[J]. Cell Signal, 2007, 19(6): 1238-1248.
[5] O'Donnell J J 3rd, Zhuge Y, Holian O, et al. Loss of p120 catenin upregulates transcription of pro-inflammatory adhesion molecules in human endothelial cells[J]. Microvasc Res, 2011, 82(2): 105-112.
[6] Reynolds A B. p120-catenin: past and present[J]. Biochim Biophys Acta, 2007, 1773(1): 2-7.
[7] 南润玲,尚培中.连环蛋白家族的新成员—p120-连环蛋白[J]. 中国普外基础与临床杂志, 2011, 18(4):462-467.
NAN Runling, SHANG Peizhong. p120-catenin-a new member of catenin family[J]. Chin J Bases Clin General Surg, 2011, 18(4): 462-467.
[8] 盖文君,姚宏,姚孟飞,等. SOX2 OCT4 β-连环蛋白 p120ctn在甲状腺癌中的表达及临床意义[J]. 中国药物与临床, 2014, 14(7): 885-890.
GAI Wunjun, YAO Hong, YAO Mengfei, et al. Expression and clinical significance of SOX2, OCT4, catenin and p120ctn in thyroid cancer[J]. Chinese Remedies & Clinics, 2014, 14(7): 885-890.
[9] Liu X, Yi C, Wen Y, et al. Interactions between MUC1 and p120 catenin regulate dynamic features of cell adhesion, motility, and metastasis[J]. Cancer Res, 2014, 74(5): 1609-1620.
[10] Zebda N, Tian Y, Tian X, et al. Interaction of p190RhoGAP with C-terminal domain of p120-catenin modulates endothelial cytoskeleton and permeability[J]. J Biol Chem, 2013, 288(25): 18290-18299.
[11] Nanes B A, Chiasson-MacKenzie C, Lowery A M, et al. p120-catenin binding masks an endocytic signal conserved in classical cadherins[J]. J Cell Biol, 2012, 199(2): 365-380.
[12] Zhu Y T, Han B, Li F, et al. Knockdown of both p120 catenin and Kaiso promotes expansion of human corneal endothelial monolayers via RhoA-ROCK-noncanonical BMP-NFκB pathway[J]. Invest Ophthalmol Vis Sci, 2014, 55(3): 1509-1518.
[13] Perez-Moreno M, Davis M A, Wong E, et al. p120-catenin mediates inflammatory responses in the skin[J]. Cell, 2006, 124(3): 631-644.
[14] Hu G, Vogel S M, Schwartz D E, et al. Intercellular adhesion molecule-1-dependent neutrophil adhesion to endothelial cells induces caveolae-mediated pulmonary vascular hyperpermeability[J]. Circ Res, 2008, 102(12): 120-131.
[15] Wang Y L, Malik A B, Sun Y, et al. Innate immune function of the adherens junction protein p120-catenin in endothelial response to endotoxin [J]. J Immunol, 2011, 186(5): 3180-3187.
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