JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 6-9.doi: 10.6040/j.issn.1671-7554.0.2014.348

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Extraction and identification of mouse islet microvascular endothelial cells (MS-1)-derived exosomes

XIAO Fang1,2,3, JIANG Weidong1, WANG Xiaohong1, QIN Aiqiong1, HAN Min1, CHEN Li2,4   

  1. 1. Department of Cadre Health Care, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Institute of Endocrinology and Metabolism, Shandong University, Jinan 250033, Shandong, China;
    3. Research Centre for Cell Therapy, Shandong University, Jinan 250033, Shandong, China;
    4. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-05-28 Revised:2014-10-22 Published:2015-01-10

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Abstract: Objective To establish a method for mouse islet microvascular endothelial cells (MS-1)-derived exosome extraction and identification. Methods Exosomes from the supernatant of MS-1 cells were collected by the combination of step-by-step centrifugations and ultracentrifugation, then characterized by transmission electron microscopy (TEM) and Western blotting. Results The extract was of round vesicle structure, surrounded by an intact double membrane, containing low electron density material inside. The diameters of exosomes ranged from 40-100 nm. The exosomes scattered or gathered into a micelles, and positively expressed CD63, CD9, and TSG101 molecules. Conclusion The extracts are proved to be exosomes and the method for extraction and identification will facilitate further clinical and basic researches.

Key words: Mouse islet endothelial cells, Exosome, Centrifugation, Isolation

CLC Number: 

  • R587.1
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