JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 41-46.doi: 10.6040/j.issn.1671-7554.0.2014.098

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Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells

ZHAO Yiyun1, HUANG Jingchun1, WANG Yuelian1, SHAO Shan1, SUN Guangping1, CUI Enmei1, ZHUO Shaoyang2, LIU Shaohua3   

  1. 1. Department of Stomatology, Third Hospital of Jinan, Jinan 250101, Shandong, China;
    2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Stomatology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-02-26 Revised:2014-12-17 Published:2015-01-10

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Abstract: Objective To explore the effects of pingyangmycin (PYM) combined with sodium hyaluronate (HA) on human lymphatic malformations endothelial cells (HLMECs) in vitro. Methods Primary HLMECs were isolated from a HLMs tissue and cultured with endothelial cell medium (ECM). By lymphatic endothelial specific marker LYVE1 and PROX1 staining, the HLMs specimen and HLMECs were identified. The proliferation effects of HLMECs treated with gradient densities of PYM (1, 10, 100, 500 μg/mL) or HA (50, 100, 300, 500 μg/mL) for 12, 24 and 48 hours, were compared with MTT assay, and the optimal concentration of PYM and HA were selected. After that, the HLMECs were treated with PYM and HA of the optimal concentration. After 24 hours, the cell proliferation and apoptosis were evaluated with MTT assay and TUNEL respectively. Results ① Both LYVE1 and PROX1 were expressed in the HLMs tissue and HLMECs. ② The optimal concentration of PYM and HA was 10 μg/mL and 100 μg/mL respectively. ③ The combination of PYM and HA was more effective than PYM alone on inhibiting HLMECs growth and promoting HLMECs apoptosis. Conclusion The combination of PYM and HA is effective for HLMECs, which can be an alternative in the clinical treatment of lymphatic malformations.

Key words: Lymphatic malformations endothelial cells, Sodium hyaluronate, Pingyangmycin

CLC Number: 

  • R782
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