JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 41-46.doi: 10.6040/j.issn.1671-7554.0.2014.098

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Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells

ZHAO Yiyun1, HUANG Jingchun1, WANG Yuelian1, SHAO Shan1, SUN Guangping1, CUI Enmei1, ZHUO Shaoyang2, LIU Shaohua3   

  1. 1. Department of Stomatology, Third Hospital of Jinan, Jinan 250101, Shandong, China;
    2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Stomatology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-02-26 Revised:2014-12-17 Published:2015-01-10

Abstract: Objective To explore the effects of pingyangmycin (PYM) combined with sodium hyaluronate (HA) on human lymphatic malformations endothelial cells (HLMECs) in vitro. Methods Primary HLMECs were isolated from a HLMs tissue and cultured with endothelial cell medium (ECM). By lymphatic endothelial specific marker LYVE1 and PROX1 staining, the HLMs specimen and HLMECs were identified. The proliferation effects of HLMECs treated with gradient densities of PYM (1, 10, 100, 500 μg/mL) or HA (50, 100, 300, 500 μg/mL) for 12, 24 and 48 hours, were compared with MTT assay, and the optimal concentration of PYM and HA were selected. After that, the HLMECs were treated with PYM and HA of the optimal concentration. After 24 hours, the cell proliferation and apoptosis were evaluated with MTT assay and TUNEL respectively. Results ① Both LYVE1 and PROX1 were expressed in the HLMs tissue and HLMECs. ② The optimal concentration of PYM and HA was 10 μg/mL and 100 μg/mL respectively. ③ The combination of PYM and HA was more effective than PYM alone on inhibiting HLMECs growth and promoting HLMECs apoptosis. Conclusion The combination of PYM and HA is effective for HLMECs, which can be an alternative in the clinical treatment of lymphatic malformations.

Key words: Lymphatic malformations endothelial cells, Sodium hyaluronate, Pingyangmycin

CLC Number: 

  • R782
[1] Perkins JA, Manning SC, Tempero RM, et al. Lymphatic malformations: current cellular and clinical investigations[J]. Otolaryngol Head Neck Surg, 2010, 142(6): 789-794.
[2] Zheng JW, Yang XJ, Wang YA, et al. Intralesional injection of Pingyangmycin for vascular malformations in oral and maxillofacial regions: an evaluation of 297 consecutive patients[J]. Oral oncology, 2009, 45(10): 872-876.
[3] Massi D, Gököz Ö. The biological significance of lymphangiogenesis in human tumours[J]. Diagnostic Histopathology, 2010, 16(6): 295-305.
[4] Waner MSJ. Hemangiomas and vascular malformations of the head and neck[M]. New York: Wiley Liss, 1999: 49-51.
[5] 雷博程, 高小波, 刘征宇, 等. 平阳霉素治疗颈部大囊型淋巴管畸形的临床疗效观察[J]. 上海口腔医学, 2013(2):233-236. LEI Bocheng, GAO Xiaobo, LIU Zhengyu, et al. Intralesional pingyangmycin injection in the management of macrocystic lymphatic malformations in cervical region[J]. Shanghai Journal of Stomatology, 2013(2):233-236.
[6] 白轶, 孙志军, 赵怡芳. 淋巴管畸形病变发生机制的研究进展[J]. 国际口腔医学杂志, 2006, 33(5): 384-386.
[7] Smith MC, Zimmerman MB, Burke DK, et al. Efficacy and safety of OK-432 immunotherapy of lymphatic malformations[J]. Laryngoscope, 2009, 119(1): 107-115.
[8] Perkins JA, Maniglia C, Magit A, et al. Clinical and radiographic findings in children with spontaneous lymphatic malformation regression[J]. Otolaryngol Head Neck Surg, 2008, 138(6): 772-777.
[9] 管宏伟, 郑晓涛. 口腔颌面部血管畸形临床治疗[J].中国伤残医学, 2013(11): 174-175.
[10] 江成鸿, 郭志辉, 黄拔瑞, 等. 平阳霉素在面颈部混合型淋巴管畸形术中及术后应用的疗效分析[J]. 齐齐哈尔医学院学报, 2011(23): 3838-3839.
[11] Yura J, Hashimoto T, Tsuruga N, et al. Bleomycin treatment for cystic hygroma in children. Nihon geka hokan Archiv fur japanische Chirurgie[J]. Nihon Geka Hokan, 1977, 46(5):607-614.
[12] 黄文雅, 肖天, 李春旺, 等. 平阳霉素聚桂醇联合治疗儿童颌面部微囊型淋巴管畸形的疗效分析[J]. 中国卫生产业, 2013(32):123-124.
[13] Nobs L, Buchegger F, Gurny R, et al. Biodegradable nanoparticles for direct or two-step tumor immunotargeting[J]. Bioconjug Chem, 2006, 17(1):139-145.
[14] 张晓燕, 金晓明, 刘晨光, 等. 阿霉素-透明质酸纳米颗粒对口腔鳞状细胞癌细胞的作用[J] . 中国组织工程研究与临床康复, 2009(25): 4985-4988.
[15] 汤朝晖, 侯春林, 顾其胜. 几丁聚糖和透明质酸钠对血管内皮细胞增殖的影响[J]. 上海生物医学工程, 2002(2):12-14.
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