JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 45-49.doi: 10.6040/j.issn.1671-7554.0.2014.113

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A new method of inducing adipose-derived stem cells into cartilage tissue

SHI Yehua, CHEN Zhipeng, MA Qikui, JU Ruihua, WANG Qirong   

  1. Department of Otorhinolaryngology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-03-04 Revised:2014-11-04 Published:2014-12-10

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Abstract: Objective To rexplore a new method of inducing adipose-derived stem cells (ADSCs) into cartilage tissue. Methods ADSCs were isolated from rat adipose tissues and characterized by cell-surface markers CD90 and CD44. The passage-6 cells were seeded in alginate beads and induced into chondrocytes in medium containing TGF-β1. After 2 weeks of culture, the cells in alginate beads were released and induced for another 2 weeks. After the induced tissues were digested by type Ⅱ collagenase, the cells were harvested and identified, and expression of type Ⅱ collagen protein was detected by cell immunohistochemistry. Results Adipose-derived stem cells exhibited a fibroblast-like morphology and were positive for typical MSC-related markers CD44 and CD90. The expression of typeⅡcollagen protein in cells of induced tissues showed that it was a new method of chondrogenic engineering to induce adipose-derived stem cells released from alginate. Conclusion Chondrogenic differentiation of cells released from ADSCs-alginate beads after 2 weeks of chondrogenic inducing is a new method of cartilage tissue engineering.

Key words: Cartilage, Adipose-derived stem cells, Alginate, Type Ⅱ collagen protein

CLC Number: 

  • R318.08
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