Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (3): 72-78.doi: 10.6040/j.issn.1671-7554.0.2017.1191

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Comparative study on biological characteristics of mesenchymal stem cells from human umbilical cord and adipose

HUANG Ping1, ZHANG Kun1,2, LI Fang1,2, HUANG Guobao3, YAN Bing4,XIAO Dongjie1,2, WANG Yunshan1,2, LIU Hua1,2   

  1. 1. Medical Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Shandong Research Center of Transplantation and Tissue, Jinan 250013, Shandong, China;
    3. Department of Burn and Plastic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    4. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2017-12-01 Published:2022-09-27

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Abstract: Objective To study the biological characteristics of mesenchymal stem cells(MSCs)derived from human umbilical cord and adipose in vitro. Methods Umbilical cord-derived mesenchymal stem cells(UC-MSCs)and adipose-derived mesenchymal stem cells(AD-MSCs)were isolated. Cell growth curve was detected using CCK-8 method, and cell surface antigens were measured using flow cytometry. The adipogenic and osteogenic differentiation assays were analyzed by Oil Red O, Alizarin Red S and Von Kossa staining. The immunomodulatory properties of UC-MSCs and AD-MSCs on lymphocytes were evaluated through the co-culture assay with PHA activated adult peripheral blood monocuclear cells(PBMCs). Results Cell growth curve showed that UC-MSCs proliferated more rapidly than AD-MSCs. Flow cytometry analysis showed that UC-MSCs and AD-MSCs were positive for CD13、CD44、CD73 and CD90, and 山 东 大 学 学 报 (医 学 版)56卷3期 -黄平,等.脐带和脂肪源性间充质干细胞生物学特性比较 \=-negative for CD34 and CD45. AD-MSCs had the stronger adipogenetic ability than UC-MSCs, but there were no difference on osteogenetic ability between these two cells. UC-MSCs has the stronger suppression effects on PBMC proliferation than AD-MSCs. Conclusion UC-MSCs are the more attractive cell population for usage in immune cellular therapy.

Key words: Umbilical cord-derived mesenchymal stem cells, Adipose-derived mesenchymal stem cell, Cell proliferation, Cell differentiation, Immunosuppression

CLC Number: 

  • R329.2
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