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Berberines effects on the proliferation, apoptosis and DNA damage and repair of human renal cell carcinoma cells
- LI Xiaofeng, DU Xiaoyi, LIU Hainan, LIU Cheng, FAN Yidong
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Journal of Shandong University (Health Sciences). 2018, 56(3):
54-59.
doi:10.6040/j.issn.1671-7554.0.2017.1202
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Objective To determine the effects of berberine(BBR)on the growth and DNA damage of human renal cell carcinoma(RCC)cells. Methods The effects of BBR on the proliferation of A498 and 786-O RCC cells at concentrations of 0-240 μmol/L were tested with CCK-8 testing kit. Based on the results of CCK-8 test, RCC cells were divided into control group(0 μmol/L), 30 μmol/L group and 60 μmol/L group. The cell apoptosis after BBR treatment was evaluated with Annexin V-FITC/PI Apoptosis Detection Kit. The expressions of proteins involved in the apoptotic pathway and DNA damage repair pathways, including pro-caspased3, cleaved-caspased3, γH2A.X and KU70, were assessed with Western blotting. Results The proliferation of RCC cells was significantly suppressed by BBR in a time- and dose-dependent manner(A498: P<0.001; 786-O: P=0.002). Cell apoptosis was obviously increased after BBR 山 东 大 学 学 报 (医 学 版)56卷3期 -李孝峰,等. 小檗碱对人肾细胞癌细胞增殖、凋亡、DNA断裂及损伤修复的影响 \=-treatment at an optimal concentration and time point. In comparison with the control group, the 30 and 60 μmol/L groups showed increased expressions of cleaved-caspase3(A498: P=0.018, P<0.001; 786-O: P=0.038, P<0.001), and γH2A.X(A498: P<0.001, P<0.001; 786-O: P<0.001, P<0.001), but decreased expression of KU70(A498: P=0.002, P<0.001; 786-O: P<0.001, P<0.001). Compared with the 30 μmol/L group, the 60 μmol/L group showed increased expressions of cleaved-caspase3(A498: P=0.020; 786-O: P=0.010), and γH2A.X(A498: P=0.002; 786-O: P<0.001), but decreased expression of KU70(A498: P<0.001; 786-O: P=0.005). Conclusion BBR can effectively suppress RCC cell proliferation, induce RCC cell apoptosis and DNA damage, and inhibit DNA damage and repair.
