Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (3): 34-40.doi: 10.6040/j.issn.1671-7554.0.2016.1640

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Effects of capsaicin on the invasion and metastasis of bladder cancer

LIU Yaxiao, SHI Benkang   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-12-09 Published:2022-09-27

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Abstract: Objective To explore the inhibitory effect of capsaicin on the invasion and metastasis of human bladder cancer 253J and RT112 cells. Methods Human bladder cancer 253J and RT112 cells were treated with 0, 100, 200 μmol/L capsaicin for 0 h, 12 h and 24 h. The viability, migration and invasion of cells were measured with MTT assay, wound healing assay, and Transwell assay, respectively. The expressions of epithelial-mesenchymal transition(EMT)markers(E-cadherin, vimentin, and N-cadherin)and nuclear erythroid-related factor 2(Nrf2)proteins were analyzed with Western blotting. Results Compared with 0 μmol/L, in the 100 and 200 μmol/L capsaicin groups, the inhibition rate of cell viability increased(P<0.01), the index of invasion decreased(P<0.01), and migration decreased(253J: P<0.01; RT112: P=0.026 or P=0.014). The expressions of E-cadherin and C-nrf2 protein increased, while the expressions of vimentin and N-nrf2 protein decreased in a dose- and time-dependent manner. Conclusion Capsaicin can inhibit the invasion and metastasis of human bladder cancer cells possibly due to mediation of Nrf2 activity.

Key words: Capsaicin, Bladder cancer, Nuclear erythroid-related factor 2, Epithelial-mesenchymal transition

CLC Number: 

  • R737.14
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