JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 41-45.doi: 10.6040/j.issn.1671-7554.0.2014.618

Previous Articles     Next Articles

Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines

GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong   

  1. Department of Pharmacology, Weifang Medical University, Weifang 261053, Shandong, China
  • Received:2014-09-17 Revised:2014-11-25 Online:2015-03-10 Published:2015-03-10

PDF (PC)

216

Abstract: Objective To investigate the expression of the cutaneous T-cell lymphoma-associated antigen 5(cTAGE5) in tumor cell lines and its biological role in the development of tumor preliminarily. Methods Expression of cTAGE5 in tumor cell lines was detected by immunofluorescence. Ovarian clear cell adenocarcinoma cell line ES-2, which was proved to be positive expression of cTAGE5, were divided into normal control group, liposome control group, siRNA transfection group and epirubicin control group(final concentration 1×10-6mol/L). The Proliferation and growth ability of ES-2 were detected by plate clone formation test and MTT test respectively, and migration ability was detected by cell scratch assay. Results cTAGE5 was expressed in cell lines got from human breast cancer, lung cancer and ovarian cancer, but little in cell lines from human stomach and liver cancer. Compared with normal control group, growth and proliferation ability of tumor cells in siRNA transfection group decreased(P<0.01), migration ability reduced (P<0.01), and proliferation ability of tumor cells in epirubicin control group decreased(P<0.01), also migration ability reduced (P<0.01). Conclusion Expression of cTAGE5 in tumor cell lines from different tissue was different. Inhibition of cTAGE5 expression can depress the proliferation, growth and migration ability of tumor cells.

Key words: Migration, Cutaneous T-cell lymphoma-associated antigen 5, Tumor cells, Proliferation, RNA interfering

CLC Number: 

  • R966
[1] 严月华, 徐丽, 吴剑波, 等.白藜芦醇对人皮肤T细胞淋巴瘤Hut-78细胞增殖和凋亡影响观察[J]. 中华肿瘤防治杂志, 2013,20(23):1807-1811. YAN Yuehua, XU Li, WU Jianbo, et al. Effect of resveratrol on inducing proliferation and apoptosis of T cell line Hut-78 cells[J]. Chinese Journal of Cancer Prevention and Treatment, 2013, 20(23):1807-1811.
[2] Usener D, Schadendorf D, Koch J, et al. cTAGE:a cutaneous T cell lymphoma associated antigen family with tumor-specific splicing[J]. J Invest Dermatol, 2003, 121(1):198-206.
[3] Eichmuller S, Usener D, Dummer R, et al. Serological detection of cutaneous T-cell lymphoma-associated antigens[J]. Proc Natl Acad Sci U S A, 2001, 98(2):629-634.
[4] Comtesse N, Reus K, Meese E. The MGEA6 multigene family has an active locus on14q and at least nine pseudogenes no different chromosomes[J]. Genomics, 2001, 75(1-3):43-48.
[5] 陈万青, 张思维, 曾洪梅, 等.中国2010年恶性肿瘤发病与死亡[J].中国肿瘤, 2014,23(1):1-10. CHEN Wanqing, ZHANG Siwei, ZENG Hongmei, et al. Report of Cancer Incidence and Mortality in China,2010[J]. China Cancer, 2014, 23(1):1-10.
[6] 姜云霞, 王冰, 崔韶晖, 等.阳离子脂质体基因载体的细胞转染研究[J]. 安徽农业科学, 2009,37(17):7862-7864. JANG Yunxia, WANG Bing, CUI Shaohui, at al. Study on the Cell Transfection of the Cationic Liposome Gene Carrier[J]. Journal of Anhui Agricultural Sciences, 2009, 37(17):7862-7864.
[7] 王博龙.乌索酸对人肝星状细胞凋亡及TGFβ_1 mRNA表达的影响[J].中国药理学通报, 2010,26(3):417-418. WANG Bolong. Effect of ursolic on apoptosis and expression of TGFβ_1 mRNA in human heportic stellate cells[J]. Chinese Phamacological Bulletion, 2010, 26(3):417-418.
[8] 王苗苗, 刁雪芹, 任桂杰, 等.嘌呤霉素敏感的氨肽酶对Taup301L诱导细胞凋亡的影响[J].山东大学学报:医学版, 2014,52(4):1-7. WANG Miaomiao, DIAO Xueqin, REN Guijie, et al. Influence of puromycin-sensitive aminopeptidase on apoptosis induced by Taup301L in cells[J]. Journal of Shandong University:Health Sciences, 2014, 52(4):1-7.
[9] 张艳丽, 刘新风, 王传新, 等.循环miR-128在结直肠癌患者血清中的表达及其对细胞迁移侵袭能力的影响[J].山东大学学报:医学版, 2014,52(8):57-62. ZHANG Yanli, LIU Xinfeng, WANG Chuanxin, et al. Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells[J]. Journal of Shandong University:Health Sciences, 2014, 52(8):57-62.
[10] Comtesse N, Niedermayer I, Glass B, et al. MGEA6 is tumor specific overexpressed and frequently recognized by patient serum antibodies[J]. Oncogene, 2002, 21(2):239-247.
[11] Boon T, Old LJ. Cancer tumor antigens[J]. Curr Opin. Immunol, 1997, 9(5):681-683.
[12] 鹿蓉, 姚振威.分子探针在疾病诊疗中的应用与展望[J].上海医药, 2014,35(13):16-22. LU Rong, YAO Zhenwei. Exploration and prospect of molecular probes for disease diagnosis and treatment[J]. Shanghai Medical & Pharmaceutical Journal, 2014, 35(13):16-22.
[13] Kaiser J. A detailed genetic portrait of the deadliest human cancers[J]. Science, 2008, 321(5894):1280-1281.
[14] Lemos RR, Oliveira DF, Zatz M, et al. Population and computational analysisof the MGEA6 P521A variation as a risk factor for familial idiopathic basal ganglia calcification (Fahr's disease)[J]. J Mol Neurosci, 2011, 43(3):333-336.
[15] Saliminejad K , Ashtari F, Kamali K. Analysis of the CTAGE5 P521A Variation with the Risk of Familial Idiopathic Basal Ganglia Calcification in an Iranian Popula[J]. J Mol Neurosci, 2013, 49(3):614-617.
[16] Oliveira JR, Sobrido MJ, Spiteri E, et al. Analysis of candidate genes at theIBGC1 locus associated with idiopathic basal ganglia calcification (“Fahr's disease”)[J]. J Mol Neurosci, 2007, 33(2):151-154.
[17] Brodaty H, Mitchell P, Luscombe G, et al. Familial idiopathic basal ganglia calcification (Fahr's disease) without neurological, cognitive and psychiatric symptoms is not linked to the IBGC1 locus on chromosome 14q[J]. Hum Genet, 2002,110(1):8-14.
[18] Bacarizo J, Camara-Artigas A. Atomic resolution structures of the c-Src SH3 domain in complex with two high-affinity peptides from classes I and II[J]. Acta Crystallogr D Biol Crystallogr, 2013, 69(5):756-766.
[19] Ren S, Peng Z, Mao JH, et al. RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings[J]. Cell Res, 2012, 22(5):806-821.
[20] 王丽, 刘晓丽, 李智慧, 等. ITSN1-S的SH3功能域对恶性胶质瘤细胞U87增殖能力的影响[J]. 中国肿瘤临床, 2013, 40(18):1089-1093. WANG Li, LIU Xiaoli, LI Zhihui, et al. ITSN1-S SH3 domains regulate human glioblastoma U87 cell proliferation[J]. Chinese Journal of Clinical Oncology, 2013, 40(18):1089-1093.
[1] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[2] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[3] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[4] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[5] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38-46.
[6] WANG Kaimin, TAN Juanjuan, YAN Zhiqiang, LI Zhiqiang. Effects of Sirtuin on hypoxia-induced proliferation of human umbilical cord-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 23-30.
[7] SHI Peikun, ZENG Beini, WU Weifang, HU Xiaoyan, MA Tianjia, ZHOU Yabin. Expression and role of Keap1 in renal cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 94-99.
[8] SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.
[9] WANG Tiantian, CHEN Jie. Expression and clinical significance of EFEMP2 in endometrial cancer tissues and cell [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 52-58.
[10] LU Heng, LIU Yanguo, LI Shuguang, CHEN Xiaokang, TIAN Qi, YI Cuihua, WANG Xiuwen. Regulation of epithelial ovarian carcinoma SKOV-3 cell migration by YKL-40 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 33-38.
[11] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[12] TIAN Siqi, LIU Riqiang, YANG Ning, WEI Wei, YANG Huawei. Effect of expression of ribosomal protein S6 Kinase 4 variants on the proliferation of breast cancer cell MDA-MB-231 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 32-36.
[13] WANG Wei, CAO Yushan, SUN Daquan, HUANG Xiaoqiong, XU Guoqiang. Role of human TIMP-2 protein in the migration and invasion of hepatocellular carcinoma cell [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 11-17.
[14] MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan. Down-regulation of α-catulin inhibits the invasion and migration of human tongue squamous cancer cell line Tscca in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 12-15.
[15] WEI Xiujuan, WU Xiuyin, TONG Dongdong, LI Jing, YANG Xiaolu, ZHANG Fenghe. Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 50-54.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd