JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 41-45.doi: 10.6040/j.issn.1671-7554.0.2014.618

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Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines

GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong   

  1. Department of Pharmacology, Weifang Medical University, Weifang 261053, Shandong, China
  • Received:2014-09-17 Revised:2014-11-25 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To investigate the expression of the cutaneous T-cell lymphoma-associated antigen 5(cTAGE5) in tumor cell lines and its biological role in the development of tumor preliminarily. Methods Expression of cTAGE5 in tumor cell lines was detected by immunofluorescence. Ovarian clear cell adenocarcinoma cell line ES-2, which was proved to be positive expression of cTAGE5, were divided into normal control group, liposome control group, siRNA transfection group and epirubicin control group(final concentration 1×10-6mol/L). The Proliferation and growth ability of ES-2 were detected by plate clone formation test and MTT test respectively, and migration ability was detected by cell scratch assay. Results cTAGE5 was expressed in cell lines got from human breast cancer, lung cancer and ovarian cancer, but little in cell lines from human stomach and liver cancer. Compared with normal control group, growth and proliferation ability of tumor cells in siRNA transfection group decreased(P<0.01), migration ability reduced (P<0.01), and proliferation ability of tumor cells in epirubicin control group decreased(P<0.01), also migration ability reduced (P<0.01). Conclusion Expression of cTAGE5 in tumor cell lines from different tissue was different. Inhibition of cTAGE5 expression can depress the proliferation, growth and migration ability of tumor cells.

Key words: Migration, Cutaneous T-cell lymphoma-associated antigen 5, Tumor cells, Proliferation, RNA interfering

CLC Number: 

  • R966
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