JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 55-59.doi: 10.6040/j.issn.1671-7554.0.2015.1101

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Expressions and significance of Th17 and Th22 cells in peripheral blood of patients with glioma

MAN Qi1, ZHANG An1, ZHANG Yuan2, FAN Mingde1, MA Daoxin3, WANG Chengwei1   

  1. 1. Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-12 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To investigate the expression and significance of Th17 and Th22 cells in the peripheral blood of patients with glioma. Methods A total of 34 patients with glioma and 24 normal controls were enrolled. The percentages of Th17 and Th22 cells in peripheral blood were measured by flow cytometry. Retinoid-related orphan nuclear receptor(RORC)and arylhydrocarbon receptor(AHR)mRNA in peripheral blood mononuclear cells were detected by reverse-transcriptional polymerase chain reaction(RT-PCR). The plasma level of interleukin-22(IL-22)was detected by ELISA. Results There was no difference between patients and healthy controls in circulating Th17 cells(P>0.05), and no difference was found between the high-grade group(WHO Ⅲ-Ⅳ)and low-grade group(WHO Ⅰ-Ⅱ)(P>0.05). The percentage of circulating Th22 cells in glioma was significantly increased compared with that in controls(P<0.05), and the difference between different grade groups was obvious(P<0.05). A positive correlation between Th22 and Th17 cells was observed in glioma(r=0.40, P=0.03), no correlation was found in controls(P>0.05). The difference of RORC and AHR between patients and controls was not significant(P>0.05, P>0.05), 山 东 大 学 学 报 (医 学 版)54卷10期 -满琦,等.脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义 \=-and no difference was found between different grade groups(P>0.05, P>0.05). The serum IL-22 level in patients was significantly increased compared with that in controls(P<0.05). However, there was no difference of serum IL-22 levels between different grade groups(P>0.05). Conclusion There exist abnormal increases of Th22 cells and IL-22 levels in the peripheral blood of glioma, and Th17 and Th22 cells may jointly participate in the pathogenesis and progression of glioma.

Key words: Th17 cells, Th22 cells, Interleukin-22, Glioma

CLC Number: 

  • R739.41
[1] Ostrom QT, Gittleman H, Liao P, et al. CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2007-2011[J]. Neuro Oncol, 2014, 16(Suppl 4): iv1-iv63.
[2] Rolle CE, Sengupta S, Lesniak MS. Challenges in clinical design of immunotherapy trials for malignant glioma[J]. Neurosurg Clin N Am, 2010, 21(1): 201-214.
[3] Galea I, Bernardes-Silva M, Forse PA, et al. An antigen-specific pathway for CD8 T cells across the blood-brain barrier[J]. J Exp Med, 2007, 204(9): 2023-2030.
[4] Harrington LE, Hatton RD, Mangan PR, et al. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type1 and 2 lineages[J]. Nat Immunol, 2005, 6(11): 1123-1132.
[5] Ivanov II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells[J]. Cell, 2006, 126(6): 1121-1133.
[6] Trifari S, Kaplan CD, Tran EH, et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells[J]. Nat Immunol, 2009, 10(8): 864-871.
[7] Duhen T, Geiger R, Jarrossay D, et al. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells[J]. Nat Immunol, 2009, 10(8): 857-863.
[8] Muranski P, Boni A, Antony PA, et al. Tumor-specific Th17-polarized cells eradicate large established melanoma[J]. Blood, 2008, 112(2): 362-373.
[9] Wang L, Yi T, Kortylewski M, et al. IL-17 can promote tumor growth through an IL-6-Stat3 signaling pathway[J]. J Exp Med, 2009, 206(7): 1457-1464.
[10] Wainwright DA, Sengupta S, Han Y, et al. The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma[J]. PLoS One, 2010, 5(10): e15390. doi: 10.1371/journal.pone.0015390.
[11] Hu J, Mao Y, Li M, et al. The profile of Th17 subset in glioma[J]. Int Immunopharmacol, 2011, 11(9): 1173-1179.
[12] Wolk K, Witte E, Witte K, et al. Biology of interleukin-22[J]. Semin Immunopathol, 2010, 32(1):17-31.
[13] Zenewicz LA, Yancopoulos GD, Valenzuela DM, et al. Interleukin-22 but not interleukin-17 provides protection to hepatocytes during acute liver inflammation[J]. Immunity, 2007, 27(4): 647-659.
[14] Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor(IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9[J]. J Immunol, 2000, 164(4):1814-1819.
[15] Zenewicz LA, Flavell RA. Recent advances in IL-22 biology[J]. Int Immunol, 2011, 23(3):159-163.
[16] Witte E, Witte K, Warszawska K, et al. Interleukin-22: a cytokine produced by T, NK and NKT cell subsets, with importance in the innate immune defense and tissue protection[J]. Cytokine Growth Factor Rev, 2010, 21(5): 365-379.
[17] Xie MH, Aggarwal S, Ho WH, et al. Interleukin(IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R[J]. J Biol Chem, 2000, 275(40): 31335-31339.
[18] Akil H, Abbaci A, Lalloue F, et al. IL22/IL-22R pathway induces cell survival in human glioblastoma cells[J]. PLoS One, 2015, 10(3): e0119872. doi: 10.1371/journal.pone.0119872.
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