Table of Content

  

16 August 2010
Volume 48 Issue 8

Articles


Articles

Renal protective effects of licorice extracts in diabetic rats

DU Hua-sheng1,2, ZHANG Rui-bin2, WANG Pu2, WANG Xiao-ping2, PANG Shu-guang3

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  1-4. 

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Objective    To investigate the protective effects of licorice extracts on the kidney of diabetic rats and the mechanism. Methods    48 Wistar rats were randomly divided into 3 groups: control group, diabetic group and therapy group. There were 16 rats in each group.  At the 8th, 12th week, detected KI, detected FBG by glucose-oxidase method, detected GHbAlc by micro-column method, detected 24h urinary albumin by Coomassie brilliant blue method, detected urine creatinine and serum creatinine by automatic biochemistry analyzer, calculated Ccr. Collected blood and made nephridial tissue homogenate, detected the content of MDA and the activity of  SOD, SeGSHPx and AR by spectrophotometer. Results    In contrast to control group, all the indexes in diabetic group and therapy group were significantly different(P＜0.05). In contrast to diabetic group, KI, FBG, GHbAlc, 24h urinary albumin, Ccr, MDA and the AR activity of blood and nephridial tissue homogenate in therapy group were obviously decreased(P＜0.05). In contrast to diabetic group, the activity of SOD and SeGSHPx of blood and nephridial tissue homogenate in therapy group were obviously increased(P＜0.05). Conclusion    Licorice extracts provide protective effects on renal function of diabetic rats, and the mechanism of this action is related to its antioxidization and ability to decrease FBG and the AR activity in renal tissue.

Prevalence of thyroid nodules in healthy population

QIAO Ling-yan1, SONG Xin-hong2, LIN Hai-yan2, GAO Ling3, ZHAO Jia-jun1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  5. 

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Objective    To assess the incidence and features of thyroid nodules in physical examinations. Methods     A total of 4687 adults without a history of thyroid disease were enrolled in the respective study. Thyroid ultrasonography was performed in all subjects and serum thyrotropin(TSH) was evaluated in 526 of them who were subdivided into two groups according to the presence or absence of thyroid nodules. And  serum TSH was compared between these two groups. Results    The prevalence of thyroid nodules was 30.12% in the studied population and increased with age increasing. Thyroid nodules were significantly more common in females than  males (34.13% vs 27.33%，P＜0.01). Spearman rank correlation analysis indicated a positive correlation between age and the occurrence of thyroid nodules(P＜0.01). Multiple nodules were more popular in elderly people, but no obvious difference was observed in the number of thyroid nodules between men and women. Most nodules were smaller than 1cm（65.79%）and the echostructure of nodules was   solid in 56.94%,  mixed in 27.12% and cystic in 15.93%  of the  cases. There was also no significant difference in serum TSH between groups with and without thyroid nodules(P＞0.05). Conclusion     The prevalence of thyroid nodules is high in healthy adults and increases with age increasing. Most nodules are found small, solitary and solid in the studied population.

Effects of oridonin on the progress of apoptosis in human glioma U251 cells and its mechanisms

LIN Tao1， LI Gang1, LIU Qing-lin1，WANG Min-qing1，WANG Jun-tao1，FENG Jin-bo2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  8-12. 

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Objective    To investigate apoptosis-induction effects of oridonin on human glioma U251 cells and its mechanisms in vitro. Methods    MTT method was used to investigate the inhibitory effects of oridonin on U251 cells. Cellular morphologic changes were observed by means of phase contrast microscopy and fluorescence microscopy with Annexin V-FITC/PI staining. Apoptosis-induction effects were evaluated by flow cytometry. Expression of bcl-2, bax and caspase-3 was evaluated by Realtime PCR. Results    Oridonin could inhibit the growth of U251 cells significantly in a time-and dose-dependent manner. Marked morphological changes of cell apoptosis were observed 24h later after the cells were exposed to oridonin. The apoptosis-induction  effects of oridonin were in a dose-dependent manner（P<0.05）. Along with the process of apoptosis，bcl-2 expression was down-regulated and bax,caspase-3 expressions were up-regulated concurrently（P<0.05）.Conclusion    Oridonin can inhibit cell growth by inducing apoptosis in U251 cells via down-regulating the expression of bcl-2 and up-regulating that of bax and caspase-3.

Protective effects and mechanism of Pioglitazone on TBI-induced  damages of  hippocampus neurons in rats

WANG Fen1, GAO Jian-xin1, QIAO Bao-hua1, LIU Ke-jing1, Guo-ying BING2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  13-17. 

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Objective    To investigate the effects and mechanism of Pioglitazone on traumatic brain injury（TBI）-induced deficits of learning memory ability and damages of hippocampus neurons in rats. Methods    Left lobus parietalis damage models were made in 24 SD rats which were randomly divided into four groups: Sham group, Vehicle group, Pio group and Pio +Ant group. Morris water maze was used to observe the changes of their learning memory behavior. On the 15th day the rats were sacrificed to make brain coronal frozen sections. NeuN,OX-42 and GFAP immunohistochemistry were used to stain neuron, microglia and astrocyte, and their numbers in hippocampus CA1,CA2 and CA3 areas were counted. Results    Morris water maze test indicated that compared with the Sham group the learning ability of the Vehicle group dropped significantly which manifested as extension of latency（P<0.01）and distance（P<0.01）; the number of neurons was decreased (P<0.01) and NeuN stain was light; the numbers of microglias(P<0.01) and astrocytes (P<0.01) were increased. The Pio group had shorter latency and distance than the Vehicle group（both P<0.05）; the number of survival neurons was increased(P<0.05) and the expression of NeuN was strengthened; thenumbers of microglias(P<0.05) and astrocytes were decreased(P<0.05). Conclusion    According to PPARγ pathway Pioglitazone can alleviate the TBI-induced inflammatory reaction in hippocampus and plays an important role in neuron protection and learning memory ability.

Effect of Qindan Capsule on expressions of vascular walll collagenⅠand mRNA in spontaneous hypertensive rats

GUO Xue-feng1, ZHANG Ji-dong1, JIANG Hong2, WANG Bo1, REN Min1, FENG Li1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  18-21. 

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Objective    To investigate the effect of Qindan Capsule (QD) on expression of vascular wall collagen Ⅰin old spontaneously hypertensive rats (SHRs), and further research into the mechanism of vascular remodeling. Methods    Thirty-two 40 week old SHRs were divided into the model group(SHR group), the QD high dosage group(SHR+QDH group), the QD low dosage group(SHR+QDL group), and the losartan group(SHR+Los group). At the same time, 16 Wistar-Kyoto (WKY) rats at the same age were taken as the normal control group(WKY control group )and QD group(WKY+QD group). All rats were administered with corresponding drugs or normal saline. After 12 weeks, the level of collagenⅠ in thoracic aorta was detected by immune-histochemical staining, and mRNA expression of collagenⅠ in thoracic aortic wall was analyzed by real-time quantitative fluorescent PCR. Results    The order in expression of collagen Ⅰfrom low to high was as follows: WKY control group, WKY+QD group, losartan group, QD high dosage group, QD low dosage group, and the model group. Compared with the normal control group,  expression of collagenⅠ in the QD group was reduced (P＜0.05). The difference in the reduction of expression of collagenⅠbetween the QD high dosage group and the losartan group was unconspicuous(P＞0.05) . Conclusions    QD could lower and inhibit expression of collagen Ⅰin the thoracic aortic wall, and modify collagen remodeling. Also, it could suppress andeven reverse thoracic aorta vascular remodeling.

Expression and significance of COX-2 in myocyte apoptosis induced by intestinal perforations due to abdominal firearm wound

XUE Hui-chao1，LI Ze-xin1，FENG De-yuan2，WU Hui2，LIU Jiang-wei2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  22-24. 

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Objective    To investigate the role of COX-2 in signal transduction of myocyte apoptosis in order to know the mechanism of heart injury after intestinal perforations due to abdominal firearm wound. Methods    A total of 42 Chang-bai piglets were randomized into 7 groups: the control group and 6 wound groups(1-, 2-, 4-, 8-, 12- and 24-h groups after wound). The model of intestinal perforations due to abdominal firearm wound was established in the wound groups. Expression of COX-2 in myocardial cells in all groups was measured with immunohistochemical staining and image analysis. Myocardial apoptosis indexes and plasma LPS levels were determined at the same time. Results    Expression levels of COX-2 in myocardial cells were significantly elevated, and myocardial apoptosis indexes and plasma LPS levels were markedly increased in the wound groups than in the control group (P＜0.05). All these changes were positively related to the changes of myocardial COX-2 activity. Conclusion    Myocardial COX-2 expression increases after intestinal perforations due to abdominal firearm wound, which can induce myocardial apoptosis and heart injury.

Oxidative effect of grape seed proanthocyanidin extract on atherosclerosis in rabbits

LIU Xiang-ju, GAO Hai-qing, QIU Jie, CHEN Huan-qin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  25. 

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Objective    To evaluate oxidative and anti-atherosclerotic effects of grape seed proanthocyanidin extract(GSPE) in rabbits by detecting malondialdehyde(MDA) and oxidized low-density-lipoprotein-cholesterol(oxLDL).  Methods    24 male New Zealand rabbits were randomized into three groups (control, model  and GSPE group), with 8 rabbits in each group. Blood samples were drawn from the middle arteries of rabbits′ ear at different time points, i.e. before the experiment, the fourth, the eighth, and the twelfth weekend of the experiment. Blood samples were analyzed for oxLDL and MDA with ELISA method. Aorta were excised and observed  morphologically through HE staining and electron microscope.  Results    At the 12th weekend, the serum oxLDL and MDA  increased significantly in model group than control group (54.26±5.33)mg/L vs (24.48±3.13)mg/L, (7.49±0.53)μmol/L vs (3.08±0.13)μmol/L; all P﹤0.01). In GSPE group, oxLDL and MDA were (45.46±4.01)mg/L and (6.90±0.59)μmol/L, respectively(P﹤0.01). Morphologically, the progress of atherosclerosis was obviously alleviated by GSPE while GSPE group was better than model group.  Conclusion    GSPE has  antioxidative effect in rabbits and it can alleviates the development of atherosclerosis significantly by inhibiting the formation of oxLDL.

Inbibitory effect of Azelnidipin on left ventricular hypertrophy in spontaneous hypertension rats

ZHANG Wei1，LI Liang1, JIANG Yao2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  28-31. 

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Objective    To explore the inbibitory effect of Azelnidipin on left ventricular hypertrophy in spontaneous hypertension rats(SHRs).  Methods    Twenty healthy SHRs were randomLy divided into the Azelnipin treatment group and the SHR group．At the same time，ten WKY rats( the WKY group) served as the normal control group. Rats in the Azelnidipin group were treated with Azelnidipin for 10 weeks and rats in the SHR and WKY groups were treated with 0.9％ NaCl of the same volume simultaneously. Ten weeks later, SBP, HR and the indexes of LVW／BW, TNF-α and NO were determined. Results    Compared with the WKY group,  SBP, LVW／BW and TNF-α of the SHR  groups had a great increase（P＜0.05）, and meanwhile NO in the serum and myocardium decreased（P＜0.05）. Compared with the SHR group, SBP, LVW/BW and NO in the serum and myocardium of the Azelnidipin group significantly increased（P＜0.05）, while TNF-αdecreased（P＜0.05）. Conclusion     Azelnipin may inhibit left ventricular hypertrophy of SHR, which may be related to the increase of NO and decrease of TNFα.

Inhibitory effect of the polysaccharide of Grifola frondosa on carbon tetrachloride-induced injury to liver cell line L-02

WANG Yu-zhuo1，XIE Shan-shan 1，SUN Tao1，ZHANG Dao-lai1，SUN Qing-ji2, SUN Yun-fan3，FENG Yu-xin1，XIN Hua1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  32. 

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Objective    To investigate the inhibitory effect of the polysaccharide of grifola frondosa (PGF) on carbon tetrachloride (CCl4)-induced injury to liver cell line L-02.  Methods    Human liver cell line L-02 was cultured and the CCl4 -induced liver cell injury model was set up . The cells were divided into the normal control group, the CCl4-damaged group and the PGF inhibitory groups (50, 100, 200 and 400μg/mL). MTT assay was used and morphology was observed to measure cell activity. The contents of alanine aminotransferase (ALT) , aspartate aminotranferase (AST) , superoxide dismutase（SOD） and malondialdehyde (MDA) were detected. The changes of mitochondrial membrane potential of L-02 were analyzed by flow cytometry. The concentration of the intracellular calcium ion was determined by Fluo3-AM. Expressions of  bcl-2 and bax proteins were measured by Western blot analysis.  Results     Compared with the CCl4-damaged group, PGF improved the relative survival of L-02, lowered activities of the AST and ALT in medium, prevented the elevation of MDA, kept the high degree of SOD, prevented the decrease of the mitochondrial membrane potential and increase of the concentration of Ca2+,  up-regulated expression of bcl-2, and down-regulated expression of bax. PGF at a concentration of 100μg/mL had the best inhibitory effect. Conclusion    PGF can significantly prevent L-02 cells from damage induced by CCl4, and the mechanism may be related to scavenging free radicals, inhibiting  intracellular calcium overload, stabilizing mitochondrial membrane potential, and regulating expressions of bcl-2 and bax proteins.

Protein nitrotyrosine and cell apoptosis in the liver and brain of hepatic encephalopathy rats

YE Qing1, RONG Lin2, WANG Xin-ting3, SHAO Li-hua2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  38-41. 

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Objective    To investigate protein nitrotyrosine and cell apoptosis in the liver and brain tissues of rat model of hepatic encephalopathy (HE). Methods      Male Wistar rats were randomly divided into HE group and control group. To manufacture the HE model, the HE group was treated with thioacetamide (TAA) by intraperitoneal injection. The control group was treated with normal saline by the same way. Then frozen sections were made from the rat liver and brain of the 2 groups. In the frozen sections, protein nitrotyrosine (3-NT) and nitricoxide synthase (NOS) was tested by immunoenzymic method of SABC, and cell apoptosis was tested by TdT-mediated dUTP nick-end labeling（TUNEL）. Cell apoptosis index was calculated. Results    Significant nitrate protein deposition was observed in the rat liver frozen sections in the HE group, while it was rare in the control group. The expression of NOS was much more in the rat liver and brain tissues of the HE group. Cell apoptosis index was significantly higher in the HE group than in the control group （P<0.01）. Conclusions    There is a close relationship between HE and protein nitration in the liver and brain tissues.

Effect of glutathione combined with platinum derivative on gastric cancer cell lines in vitro

XU Yan-yan1， YU Hui-min2, JIANG Na3

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  42-46. 

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Objective    To explore the effect of glutathione(GSH) combined with oxaliplatin(L-OHP) and cisplatin(CDDP) on the proliferation and apoptosis of gastric carcinoma cell line SGC-7901 cells.  Methods    The cells in five groups were treated according to the following drug administrations: GSH, CDDP, L-OHP, GSH combined with CDDP, and GSH combined with L-OHP. Cell proliferation was evaluated by SRB assay, inverted microscope and fluorescent microscope were used to observe morphological changes, and the distribution of cell cycle and the rate of apoptosis were observed by flow cytometery respectively. Results    GSH combined with L-OHP and CDDP obviously inhibited the proliferation of SGC-7901. The combination group of GSH and CDDP had no significant difference compared with the CDDP group (P>0.05), while the combination group of GSH and L-OHP had a significant difference compared with the L-OHP group (P<0.05). Whether cells were treated by single platinum derivative or by combination of GSH and platinum derivative, cancer cells showed the morphological change of apoptosis when observed microscopically. Flow cytometry analysis showed that the combination group of GSH and L-OHP and the L-OHP group had reduced number of cells in S phase and showed an apparent peak of apoptosis. However, the L-OHP group showed more obvious growth inhibition to SGC-7901 than the combination groups. Conclusion    GSH dose not reduce the effect of CDDP on inhibiting the growth of SGC-7901, but it reduces the effect of L-OHP on SGC-7901 cells, and reduces the apoptosis of gastric cancer cells.

Pharmacokinetics of levofloxacin in rabbit blood and tissue fluid after intravenons injection

SHENG Mei-yan1，2，LIU Ming-tao1，3，ZHANG Yun1， 3，WEN Kun2, WANG Ben-jie4, LI Yu1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  47. 

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Objective    To measure blood and tissue fluid concentrations of levofloxacin (LVFX) in rabbits and calculate the drug and pharmacokinetic parameters after intravenous injection， and then provide the basis for the transition of common pathogens such as Pseudomonas aeruginosa in vitro studies to in vivo. Methods    The rabbit cage models were established, and then the rabbit blood and  tissue fluid concentrations of LVFX at different times were measured by high performance liquid chromatography(HPLC). The pharmacokinetic parameters were calculated. Results    After 10mg/kg LVFX was intravenously injected in rabbits，the peak concentration (Cmax ) of LVFX in blood and tissue fluid was (8.27±0. 9) and (2.48 ±0. 3)mg／L ; the half-life ( T1/ 2β) of various concentrations in blood and tissue fluid was (1.06±0.1) and (3.63±1.3)h; the apparent volume of distribution was （1.55±0.3）and（4.89±2.1）L/kg, respectively. Conclusion    LVFX shows good penetration when administered by intravenous injection in the tissue fluid. Both the long half-life of LVFX and small fluctuation of drug concentration could be helpful to maintain therapeutic levels in tissue fluid.

Effects of ⁹⁹Tc-MDP on PGE1 and PGE2 in rats of adjuvant arthritis

ZHANG Yu-jun, DING Feng，WANG Chun-xiao， LI Xing-fu，YU Xiao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  50-53. 

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Objective     To observe the effects of ⁹⁹Tc-MDP (technetium-99 conjugated with methylene diphosphonate) on adjuvant arthritis in rats, and study its anti-inflammatory mechanism. Method    The AA(adjuvant arthritis) rat model was established by intradermal injection of complete Freud′s adjuvant. Thirty male Wistar rats were divided randomly into three groups: control group, adjuvant arthritis group and ⁹⁹Tc-MDP treatment group. In treatment group,  ⁹⁹Tc-MDP （2.5mL/kg）was administered  through caudal vein on the 10th day and repeated 15 days later after arthritis was induced. Same volume normal saline was given to the other two groups under the same protocol. Then, left-right diameter of left hind ankle, arthritis index of each rat were recorded while  PGE1 and PGE2 in peripheral blood were measured by ELISA. Results    ①The leftright diameter of left hind ankle in both  control group and  ⁹⁹Tc-MDP treatment group reduced with time, but the diameter in  ⁹⁹Tc-MDP treatment group significantly reduced（P＜0.05）than that of AA group； The arthritis index of AA group and ⁹⁹Tc-MDP treatment group both decreased with time, but the index  decreased much more significantly（P＜0.05）in the latter. ②There were no significant differences among all groups on the level of PGE1（P＞0.05）,and the level of PGE2 of the AA group and  ⁹⁹Tc-MDP treatment group were notably higher than the control（P＜0.05）. The PGE2 level of control l group was much higher than that of ⁹⁹Tc-MDP treatment group（P＜0.05）. Conclusion      ⁹⁹Tc-MDP  can reduce the left-right diameter of hind ankle and arthritis index in adjuvant arthritis rats.  ⁹⁹Tc-MDP may have a certain anti-inflammatory effect on adjuvant arthritis rats.

Effects of estrogen on the disease development in the collagen-induced arthritis  animal models

WEI Chao1, WU Wei-ping1, ZHAO Yan1, WANG Lin1, YAN Xin-feng2, CHANG Xiao-tian1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  54-59. 

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Objective    To investigate the effects of estrogen on the disease development and the expressions of peptidylarginine deiminase 4 (PADI4) and thioredoxin domain containing protein 5 (TXNDC5) in collagen induced arthritis (CIA) models. Methods    Twenty-nine male Lewis rats were randomly divided into four groups: the healthy control group (group H, n=6）, the CIA group (group C, n=8), the CIA group treated with estrogen (group T, n=8) and the estrogen group（group E, n=7). CIA was induced subcutaneously with bovine type Ⅱcollagen (CⅡ) and estrogen treatment was operated during CⅡsensitization. ELISA was used to measure the levels of TNFα, IL-1β and VEGF in serum samples. Immunohistochemisty, Western-blot and real-time PCR were applied to detect the expressions of PADI4 and TXNDC5 in synovial membranes. Results    Compared with group C, the onset of CIA significantly delayed for 6 days (P=0.045) with no effects on inflammatory severity and incidence rates of the disease in group T; the serum levels of TNFαand IL-1βwere not evidently altered, but the level of VEGF was considerably increased (P=0.023). Synovial tissues in group T showed more hyperplasia with angiogenesis, in which PADI4 was mainly expressed in the lining and infiltrated inflammatory cells of synovium and TXNDC5 was mainly presented in the lining of synovium and endotheliocytes of capillaries. Estrogen pronouncedly stimulates the expression of TXNDC5 (P<0.05) rather than the expression of PADI4. Conclusion    Although estrogen can delay the onset of arthritis, it may stimulate angiogenesis in rheumatoid arthritis synovium via the up-regulation of TXNDC5.

Effect of Dipsacaceae and Salvia on orthodontic teeth movement

MEI Yinsheng1，2, JIA Yulong1， ZHANG Lina1， JIN Shumei1， ZHANG Jun1，3

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  60-64. 

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 Objective    To detect and compare the effect of the traditional Chinese drugs, Dipsacaceae and Salvia, on the reconstruction of paradentium in orthodontic teeth movement. Methods    72 female, 8-week-old SPF Wistar rats were selected and the animal models for orthodontic tooth movement were established. The rats were divided into three groups randomly: Dipsacaceae group, Salvia group and control group. Each group had 24 rats. Dipsacus asperoides decoction(6g/kg), Salvia decoction(6 g/kg)  and 3mL NS were drenched daily to the rats in the Dipsacaceae group, Salvia group and  control group respectively.  Stability of the appliances were checked everyday and the applied force was raised by adjusting the springs every 7 days. The rats were executed in batch on the 7th、14th、21st、28th day by peeling off the skulls. Distances of the teeth movement were measured and slices of the periodontium of the maxillary first molar were observed under optical microscope. Data were analyzed with PASWstatistics18. Results    Teeth movement in the Dipsacaceae group and Salvia group were greater than control group（P＜0.05）while  no significant difference（P＞0.05）was found between them. Light microscope revealed the number of osteoclasts in all 3 groups increased rapidly at the beginning but flatly later. Osteoclasts in  Dipsacaceae and Salvia group showed much more increase than the control （P＜0.05）, however, no significant difference existed between them(P＞0.05). Though alveolar bone density showed the trend of decrease in all 3 groups, Dipsacaceae and Salvia group decreased slowly(P＜0.05) and nosignificance was discovered between the two(P＞0.05). Conclusion    Salvia and Dipsacaceae decoction can promote the multiplication and differentiation of the osteoclasts in rats, benefit  alveolar bone reconstruction and accelerate orthodontic tooth movement.

Regulation of Toll-like receptor 4  by ISO-1 in HUVECs

HAN Ye-ming, JANG Shi-qin, ZHANG Chao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  65-69. 

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Objective    To study the effect of ISO-1 ［(S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester］, a specific antagonist of MIF (Macrophage migration inhibition factor),  on TLR4 (Toll-like receptor) activities in HUVEC (Human umbilical vein endothelial cells), and to provide an experimental evidence for  the treatment of  TLR4-related diseases in which ISO-1 is targeted at. Methods   HUVECs were cultured and divided into 4 groups: LPS (lipid polysaccharide) group, ISO-1 pretreatment group, LPS+ISO-1 group and Medium control group.  Effects of ISO-1 on TLR4 expression, TNFα (tumor necrosis factor) and NOS (nitric oxide synthase ) secretion were investigated with RT-PCR, immunofluorescense staining, radioimmunoassay and enzyme-chemical reactions. Results    The expression of TLR4 in HUVEC was obviously inhibited by pretreatment with high concentration of ISO-1, especially in 25 and 50μmol/L groups (P＜0.05).  Low expression of TLR4 was also detected at 0.5, 1, 2 and 3h after the pretreatment with 50μmol/L ISO-1, in which the lowest appeared at 1h (P＜0.05) . Immunofluorescense showed low level TLR4 in  normal HUVEC, however, the expression could be enhanced by LPS (10ng/mL). TLR4 expression, TLR4-induced TNFα and NOS secretion were markedly suppressed 1h later after the pretreatment with 50μmol/L ISO-1, and such inhibition was does-dependent (P＜0.05). The effective time of ISO-1 was short and the maximal was at 1h. Conclusion    ISO-1 can exert the inhibitory effects through down-regulating the TLR4 activities of HUVEC, which suggests that ISO-1 may be  a potential target-agent in the treatment of TLR4-related diseases, such as atherosclerosis and inflammation.

Intervention effects of Chinese herbal compound on the behavior and growth of mice with epilepsy induced by pentetrazole

WANG Bin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  70-73. 

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Objective    To observe the effect of AAP, a Chinese herbal compound, on the behavior and growth of mice with seizures induced by pentetrazole (PTZ). Methods    Sixty healthy mature male Kunming mice were randomly divided into 6 groups (n=10 in each group): the control group (CK group), the model group (PTZ group), three AAP groups with large, medium and small doses (AAPl group, AAPm group and AAPs group), and sodium valproate group (VPA group). The control group and model group were given intragastric administration of distilled water (4mL/kg·d)； the three AAP groups were given intragastric administration of AAP at doses of 14.8g/kg ,7.4g/kg and 3.7g/kg respectively； and the VPA group was given intraperitoneal injection of sodium valproate (20mg/kg·d). After 7 days of treatment, the seizure was induced by intraperitoneal injection with pentylenetetrazol（95mg/kg）in all groups other than the control group. Behavioral observation was conducted with Yuhas 5grade assessment during the establishment of the models, and the weight gain values were recorded. Results    Compared with the model group, the latent period was prolonged and time span of the seizure was reduced in the AAPs group (both P＜0.05).The weight gain value in all the three AAP groups increased significantly (P＜0.05) compared with that in the VPA group. Conclusion    AAP can extend the latency, reduce the duration of epileptic seizures induced by PTZ, and increase the weight gain, so it has a positive anticonvulsant effect on mice.

Precisely mapping the antigenicity and immunogenicity on N-terminal regions of  the dengue virus non-structural protein 1 and detecting DENV-immunized animal serum samples using an epitope-based peptide antigen

CHEN Yue, PAN Yu-xian， QIU Li-wen， DING Xi-xia, CHE Xiao-yan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  74-79. 

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Objective     To precisely determine the antigenicity and immunogenicity on N-terminal regions of DENV NS1 and develope serodiagnosis of DENV infection using an epitope-based peptide antigen. Methods    The synthesis peptide sequences of N-terminal regions of DENV NS1 were derived from the four serotype DENV standard strains and named D-1 P1, D-2 P1, D-3 P1 and D-4 P1. ELISA was performed to analyze antibody responses to these peptides from monoclonal antibodies (MAbs) with dengue serotype specificity as well as antisera from mice immunized with the dengue virus serotypes. The specific reactivity of peptides with corresponding MAbs was further conformed by competitive inhibition experiments. The degree of conservation and variability of N-terminal regions of DENV NS1 within DENV isolates which were reported in Pubmed were analysised by Bioinformatics. Results    Six DENV-1 serotype-specific MAbs (5A48A3, 5A65A2, 5B29A1, 5B71A8, 5C29A3, and 5E68A17) showed a strong reaction with D-1 P1. Six DENV-2 serotype-specific MAbs (5D21A1, 5E19A5, 5A62A12, 5A70A4, 5E30A5 and 5E48A15) showed a strong reactionwith D-2 P1. D-1 P1 only reacted with all 3 sera from DENV-1-immunized mice. The other peptides were not different from sera from mice immunized with all four dengue virus serotypes. Alignment revealed that the conservation of 100% homology for N-terminal regions of DENV-1,2,3,4 NS1 was 98.96%, 89.09%, 83.58% and 57.81%, respectively.  Conclusions    N-terminal regions of Dengue virus-1 non-structural protein 1 contained an immunodominant serotype epitope and N-terminal regions of Dengue virus-2 non-structural protein 1 contained a serotype epitope. D-1 P1-based peptide antigen will be useful for serologic diagnosis of DENV-1 infection. These novel serotype B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and serodiagnosis assays.

Effect of  tyrosine kinase Etk/BMX on nasopharyngeal carcinoma cells

HAN Xiao-bing1, YU Hong-bo2, WANG Yu-jun3

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  80. 

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Objective    To study the relationship between the tyrosine kinases Etk/BMX and nasopharyngeal carcinoma (NPC) cell proliferation. Methods    Wild type Etk/BMX pcDNA3.1Etk was recombinated into low expression of Etk/BMX NPC cell line sune-1 using plasmid 2000, and thus the sune-wt cell line which Etk was highly and stably expressed was established, and cells which transfected by pcDNA3.1 empty plasmid (sune-vector) was used as controls.The transfection rate was examined using flow cytometry and then the cells were detected  by a clone plate experiment and inoculated  into nude mice  to observe tumorigenesis. Results    The sune-wt cells with high Etk expression were successfully obtained, showing an expression rate of (75.5±2.34)%. Clone plate experiment showed that grow rate of sune-wt cells was higher than that of sune-vector and sune-1 cells. sune-wt had a higher rate of growth and tumorigenesis than both sune-vector and sune-1 cells in nude mice，but there were no significant differences in tumor growth between sune-vector and sune-1 cells. Conclusion    Etk/BMX may increase the oncogenicity and cell proliferation in nasopharyngeal carcinoma.

Diagnostic value of DTI in patient with unilateral middle cerebral artery occlusion on 3.0T MR

MENG Xiang-shui1, CHAI Xiang-jun2, MA Xiang-xing1, WANG Qing1, ZHANG XIAO-ming1, WANG Qian1, LI Chuan-fu1, HOU Jin-wen1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  83-87. 

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Objective    To evaluate the diagnostic value of the diffusion tensor imaging (DTI) with different b values in patients with unilateral middle cerebral artery occlusion. Methods    Thirty-six patients with severe stenosis or occlusions of unilateral middle cerebral artery underwent DTI imaging study with different b values（3100～400s/mm2）on 3.0T MR. ADC and FA values in white matter of affected hemicerebrum were analyzed by comparing with that of the contralateral healthy part. Results    In affected hemicerebrum, ADC values with b values from 33100 to 1000s/mm2 were bigger than that of the healthy(P＜0.05), while FA values with b values from 33100 to 1300s/mm2 were smaller(P＜0.05). There were no significant difference in ADC values (b values from 700 to 400s/mm2) and FA values (b values from 1000 to 400s/mm2) between the two sides(P＞0.05). Conclusion    Abnormal diffusion can be detected by ADC and FA values more sensitively with high b values than conventional b values when the conventional MRI failed in the patients with unilateral middle cerebral artery severe stenosis or occlusion.

Diagnostic model of lung cancer established with tumor markers and artificial neural network

JIANG Man1， GENG Ling-yun1， CAI Hong-fei1， WANG Ming-ming2， WANG Dong-fang1, WANG Jia-lin3， HAN Ming-yong1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  88. 

Abstract ( 1193 )   PDF (544KB) ( 270 )   Save

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Objective    To establish a diagnostic model for lung cancer with artificial neural network(ANN) and tumor markers. Methods    Serum levels of CYFRA21-1、NSE、TPS、sIL2R、CEA、CA242 and p53-Ab were detected in 86 lung cancer patients and 80 healthy subjects. The tumor markers were evaluated by area under curves anda diagnostic model with artificial neural network was further established. Results    The levels of CYFRA-211、NSE、TPS、SIL-2R、CEA、CA242 and p53-Ab were detected; The diagnostic mode of lung cancer was built with artificial neural network. Accuracy, sensitivity and specificity of this model were84.1%,  86.3% and 94.8%, respectively, in the diagnosis of lung cancer. Conclusion     ANN model of tumor markers has a high sensitivity and specificity in diagnosis of lung cancer.

Comparative study of multislice spiral computed tomography and pathology in relationship between solitary pulmonary nodule and bronchus

ZHANG Jing-dong， MA Xiang-xing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  91-95. 

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Objective    To evaluate the relationship between solitary pulmonary nodule (SPN) and bronchus and its value in predicting the nature of nodule. Methods    66 SPN cases (29 benign and 37 malignant tumors) with clear diagnosis were retrospectively studied, All patients were scanned by multi-slice spiral computed tomography ( MSCT) and the images were reformed by multi-planar reformation(MPR) in the workstation to show SPNbronchus relationship from various angles. SPN- bronchus relationship was analyzed and compared with the examination result of microscopic specimens. Results    The SPN and bronchus was related in 51 of the 66 cases, including 18 (62.1%) benign nodules and 33 (89.2%) malignant nodules. There was a significant difference bwteen the benign and malignant groups (P＜0.01). The SPNbronchus relationship was identified as five types: typeⅠ: the bronchus was abruptly obstructed at the edge of SPN; typeⅡ: the bronchus penetrated into SPN with tapered narrow and interruption; type Ⅲ: the bronchus ran through the whole SPN; typeⅣ: the bronchus ran at the periphery of SPN; and typeⅤ: the bronchus converged on the SPN. The SPN-bronchus relationship in most malignant nodules belonged to typesⅡ,Ⅲ and Ⅴ. TypesⅡ and Ⅲ was more showed in adenocarcinoma. There was a significant difference between malignant and benign tumors in typesⅡand typeⅤ(P＜0.01). Conclusion    MSCT volume scan and MPR can clearly show the relationship between the SPN and bronchus, and the relationship is valuable in determining the nature of differentiation of SPN. Certain patterns of SPN- bronchus may reflect definite pathologic changes of SPN.

Three-dimensional anatomic study of superior mesenteric artery stem and adjacent structures of adults on multislice CT

CUI Yi1, HE Jing-zhen1, WANG Lei2, WANG Qing1, HOU Jin-wen1, MA Xiang-xing1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  96-99. 

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Objective    To measure the caliber extent of superior mesenteric artery (SMA) stem and adjacent structures in different genders and ages. Methods    The data from 260 patients (140 females, 120 males) were acquired. The caliber of SMA stem was measured and the adjacent anatomic structures were identified in patients who underwent abdominal contrast-enhanced multi-slice helical CT (MSCT). Sagittal imaging of SMA was acquired by thin slab maximum intensity projections(TSMIP) postprocessing technique. Results    The diameter of SMA was（0.68±0.10）cm, the distance between SMA and celiac artery (CA) was (1.55±0.33）cm， the distance between SMA and inferior mesenteric artery (IMA) was（6.69±0.88）cm, and the angel between SMA and abdominal aorta (AA) was（59±18）°. The statistical difference between males and females was observed in the diameters of SMA, CA and AA（P＜0.05）. There was no statistical difference between males and females in the distances of SMA and CA, the distance of SMA and IMA, and the angel of SMA and AA（P＞0.05）. The diameter of CA had no significant difference（P＞0.05）, but there was statistical difference in the diameter of AA in different ages （P＜0.05）. Conclusion    SMA stem and adjacent anatomical structures were delineated limpidly by MSCT and the extent of diameter could be measured exactly.

Relationship of expression level of serum IL-8  with HCV genotypes and antivirus treatment in patients with chronic hepatitis C

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  100-102. 

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Objective    To study the relationship of expression level of serum interleukin-8 (IL-8)  and HCV genotypes in patients with chronic hepatitis C and observe the change of serum IL-8 level after antivirus treatment.  Methods    HCV genotypes were determined using DNA chip assay in 74 chronic hepatitis C patients. Serum IL-8 of these patients was determined using bi-antibody sandwich ABC-ELISA assay. Levels of IL-8 in chronic hepatitis C patients with different genotypes were compared. The change of IL8 level after 12 weeks of antivirus treatment was observed. Results    Levels of serum IL-8 in chronic hepatitis C patients and healthy controls were (42.95±50.00)pg/mL and( 11.06±1.39)pg/mL, respectively（P＜0.01），and in patients infected with genotype 1b and genotype 2a were ( 52.72±60.67)pg/mL and (26.93±13.97)pg/mL, respectively（P＜0.05）. After 12 weeks of antivirus treatment, the serum IL-8 level in patients with undetectable HCV RNA decreased from (41.20±25.89)pg/mL to (23.89±10.59 )pg/mL（P＜0.01）.  Conclusions    The serum level of IL-8 in patients with chronic hepatitis C significantly increases compared with that in healthy controls. The IL8 level in patients with genotype 1b is higher than that in patients with genotype 2a. The IL-8 level in patients with undetectable HCV RNA significantly decreases after antivirus treatment.

Clinical efficacy of magnesium isoglycyrrhizinate in the management of virus hepatitis with hyperbilirubinemia

GUO Qian, NONG Cun-li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  103-106. 

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Objective    To evaluate the clinical efficacy of magnesium isoglycyrrhizinate in the management of virus hepatitis with hyperbilirubinemia. Methods    117 patients with virus hepatitis with hyperbilirubinemia were randomly divided into the therapy group and the control group. Besides routine therapy, patients in the therapy group received management of magnesium isoglycyrrhizinate. The serum biochemical parameters were determined in all patients at the onset and end of treatment. Results    Four weeks after the treatment, the total effective response was 82.5% in the therapy group and 70.4% in the control group with a significant difference (P=0.004). After four week follow up, the total effective response was 88.9% in the therapy group and 53.7% in the control group with a significant difference (P<0.001). The treatment with magnesium isoglycyrrhizinate was effective in reducing liver fibrosis and without significant side effects. Conclusion    Magnesium isoglycyrrhizinate is effective for virus hepatitis with hyperbilirubinemia.

Endoscopic resection of esophageal mesenchymal tumors 

ZHANG Xiaoqian， ZHANG Hongmei， SUN Xiuju， FENG Yuguang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  107-110. 

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Objective    To explore the methods of endoscopic dissection of esophageal mesenchymal tumors and to evaluate their safety and efficiency for the treatment  of esophageal mesenchymal tumors. Methods    Endoscopic electrosection or submucosal dissection was performed in 39 cases of esophageal mesenchymal tumors. The tumors which originated from mucosal muscularis determined by endoscopic ultrasonography (EUS) and were less than 1.0cm in diameter were resected by snare electrosection. The tumors which originated from mucosal muscularis and were more than 1.0cm in diameter, which were too flat to snare, or which originated from muscularis propria determined by EUS were resected by endoscopic submucosal dissection ( ESD). Results    Comeplete resection rate: the tumors originating from mucosal muscularis in all the 28 cases were comepletely resected. In the 11 cases whose tumors originated from muscularis propria, much bleeding was not successfully controlled by the endoscopy and was then treated by surgery in 1, the tumor adhered to the muscularis propria and was not resected by the endoscopy in 2, and the comeplete resection rate was 72.7%. There was a significant difference in comeplete resection rate between the two groups（P＜0.05）. Complications: 1 case had bleeding and no one had perforation in the 28 cases whose tumors originated from mucosal muscularis, and the complication rate was 3.5%; 2 cases had bleeding and 2 cases had perforation in 11cases whose tumors originated from muscularis propria, and the complication rate was 36.3%. There was a significant difference in complication rate between the two groups（P＜0.05）. Conclusion    It is safe and effective to conduct endoscopic resection for esophageal  mesenchymal tumors originating from mucosal muscularis.  For tumors originating from muscularis propria, the comeplete resection rate decreases and the complication rate increases, and they should be treated by surgery.

Experience of emergency surgery on ruptured abdominal aortic aneurysm（a report of 19 Cases）

ZHONG Zhen-yue , WANG Mo, GAO Bin-bin, DONG Dian-ning, ZHANG Shi-yi , SUN Yan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  111. 

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Objective    To explore  diagnosis, treatment and factors affecting the prognosis of ruptured abdominal aortic aneurysm（RAAA）. Methods    Between March 2003 and February 2009,  clinical data of 19 patients (15 males  4 females ; age range 5082 years, mean age 64.7 years) with RAAA below the level of renal arteries treated with emergency surgery in Shandong Provincial Hospital were analyzed retrospectively. All of the patients were subjected to sudden acuteness bellyache and ensued shock . Among them,  2 cases had a history of AAA and 8 cases had pulsating abdominal masses.  Concomitant diseases included 7  primary hypertension, 3 coronary heart disease and 1 emphysema. All  the patients underwent emergency operation: The ruptures of the abdominal aorta below the level of renal arteries were obstructed by using clamp ring or transluminal balloon according to the conditions of each patient. The artificial vascular graft was taken after the hemorrhage were stopped. After the operation, every patient was followed up for an average of (24±2) months. Results    3 (15.8%) patients died within 30 days after the operation. The causes of death included acute myocardial infarction (1 case) and multiple organ failure (2 cases). Early complications consisted of acute renal failure (4 cases), lung infection (2 cases), and diarrhea (3 cases). During follow-up, no death, anastomotic false aneurysm, graft infection, intravascular thrombus formation or other complications ever occurred. Conclusion    Early diagnosis, emergency surgery to stop bleeding, intensive perioperative management  are the keys to reduce the mortality of RAAA.

Effectiveness of   lifestyle intervention in nonalcoholic fatty liver disease

ZHANG Rong1,2， WU Feng-yun2， XU Gui-fa1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  113-116. 

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Objective    To evaluate therapeutic effectiveness of lifestyle intervention in non-alcoholic fatty liver disease. Methods    105 cases of  non-alcoholic fatty liver patients were enrolled.  Lifestyle interventions was applied to all of them, including learning knowledge of health, dietary control and exercises .There were 64 mild ,35 moderate  and 6 severe cases  diagnosed by ultrasonic tomography. Triglyceride (TG), total cholesterol（TC）, HDL-cholesterol (HDL) and LDL-cholesterol(LDL) were also measured. After 12 months of therapy, the effectiveness was finally evaluated. Results    After 12-month lifestyle intervention, 40 mild fatty liver recovered to normal（P＜0.01) while among moderate cases, 28 turned into mild and 3 recovered to normal（P＜0.01). 3 severe cases became moderate（P＜0.05）. And TG、TC、LDL decreased significantly. Conclusion    Lifestyle intervention can effectively reduce the morbidity rate and increase the recovery rate in non-alcoholic fatty liver patients.

Effect of nonpharmacotherapy in decreasing the levels of serum lipid in simple hyperlipidemia patients

ZHANG Min1，2, XU Gui-fa1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  117-119. 

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Objective    To investigate a non-pharmacotherapy program for patients with simple hyperlipidemia diagnosed in health examination and assess its effectiveness. Methods    197 patients was divided into two groups randomly: the experimental group (99 cases) and the control group (98 cases). The experimental group was given non-pharmacotherapy once a week and the therapy lasted for 6 weeks. The content of non-pharmacotherapy consisted of: a) teaching related knowledge about hyperlipidemia; b)  guiding healthy life-style, such as diet and exercise. Patients were followed up every week. The control group didn′t undergo any  non-pharmacotherapy program. A selfcompletion questionnaire on hyperlipidemia-related knowledge, questionnaires on diet and exercise were obtained and serum TG, TC, LDL-C and BMI were measured at entry point and at the end of 6-month study. Results    The hyperlipidemia-related knowledge and weekly physical exercise time of experimental group increased while the daily oil intaking and BMI decreased. The mean of serum TG, TC and LDL-C of experimental group lowered significantly comparing to control group. The TG lowered from(2.81±1.32)mmol/L to(2.06±0.91)mmol/L，TC from(6.67±0.93)mmol/L to(5.49±0.95)mmol/L，LDL-C from(4.69±0.89)mmol/L to(3.83±1.02)mmol/L， and all the differences above were significant. The declining of serum lipid was related to the increasing of hyperlipidemia-related knowledge, exercise frequency and  the decreasing of oil intaking. Conclusion    Non-pharmacotherapy is to be effective for the patients with simple hyperlipidemia and it can be used as basic therapy.

Fingerprint of Shandong trueborn Pinellia ternata(Thunb.) Breit. analyzed by HPLC

LI Bin, CHENG Xiu-min, ZHOU Yong-yan, LOU Hong-xiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  120-123. 

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Objective    To establish a high-performance liquid chromatography (HPLC) fingerprint of Shandong trueborn Pinellia ternata (Thunb.) Breit. for quality control.  Methods    HPLC was used by ODS column(4.6mm×250mm, 5μm) with the gradient elution solvert system composed of acetonitrile-water. The flow rate was 1.0mL/min, and the detection wavelength was 260nm. The HPLC fingerprints were compared by the similarity evaluation software and SPSS software.  Results    The HPLC fingerprint of Shandong trueborn Pinellia was established, and methods established for Pinellias conformed to the technical standards in fingerprint spectrum.  All the samples from Shandong Province were similar and were clustered into one group, and they were different from the commodity pinellia.  Conclusion    The established HPLC fingerprint has desirable accuracy, repeatability and stability. It can be used for the quality control of Pinellia ternata (Thunb.) Breit.       

Effects of Shenqi Fuzheng Injection Powder on immune function of mice

ZHANG Cheng-mei1, ZHANG Cheng-e2, XIN Qing3, WU Chuan-tao1, YIN Zhi-sheng1， YU Xue-hui1， LIU Zhao-ping1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(8):  124. 

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Objective    The objective of this paper is to investigate the effects of Shenqi Fuzheng Injection Powder on immune function and immune regulation of mice after intravenous injection. Methods    Each test needs 50 KM mice (25 male and 25 female).These 50 KM mice were randomly divided into five groups (Group Veh, Group 20mg/10g, Group 40mg/10g， Mand 80mg/10g of Shenqi Fuzheng Injection Powder as well as Group 0.8mL/10g of Shenqi Fuzheng Injection)according to their body weight. After being treated intravenously for 7 days, detected the immune parameters like the index of organs， the number of hemolysis plaque, the level of serum hemolysin, the delayed hypersensitivity， the transform and proliferation of T lymphocytes， the index of carbon clearance as well as the cyto activity of NK cells. Results    Compared with Group Veh, there were significant statistical differences（P< 0.05 or P<0.01）of the index of organs， the number of hemolysis plaque, the level of serum hemolysin,the delayed hypersensitivity， the transform and proliferation of T lymphocytes， the index of carbon clearance as well as the cyto activity of NK cells were found in the group of Shenqi Fuzheng Injection Powder of 40, 80mg/10g and Shenqi Fuzheng injection of 0.8mL/10g after the intravenous administration; while compared with Group Veh, the index of carbon clearance and the cyto activityof NK cells also showed significant statistical differences（P<0.05）in the group of Shenqi Fuzheng Injection Powder of 20mg/10g after the intravenous administration. Conclusion     From the experimental results, it can be speculated that Shenqi Fuzheng Injection Powder can enhance the non-specific and specific immune function on mice, and the immune function of the organism can be effectively improved.