JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (8): 8-12.

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Effects of oridonin on the progress of apoptosis in human glioma U251 cells and its mechanisms

LIN Tao1， LI Gang1, LIU Qing-lin1，WANG Min-qing1，WANG Jun-tao1，FENG Jin-bo2   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China；
    2. Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2010-06-21 Online:2010-08-16 Published:2010-08-16

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Abstract:

Objective    To investigate apoptosis-induction effects of oridonin on human glioma U251 cells and its mechanisms in vitro. Methods    MTT method was used to investigate the inhibitory effects of oridonin on U251 cells. Cellular morphologic changes were observed by means of phase contrast microscopy and fluorescence microscopy with Annexin V-FITC/PI staining. Apoptosis-induction effects were evaluated by flow cytometry. Expression of bcl-2, bax and caspase-3 was evaluated by Realtime PCR. Results    Oridonin could inhibit the growth of U251 cells significantly in a time-and dose-dependent manner. Marked morphological changes of cell apoptosis were observed 24h later after the cells were exposed to oridonin. The apoptosis-induction  effects of oridonin were in a dose-dependent manner（P<0.05）. Along with the process of apoptosis，bcl-2 expression was down-regulated and bax,caspase-3 expressions were up-regulated concurrently（P<0.05）.Conclusion    Oridonin can inhibit cell growth by inducing apoptosis in U251 cells via down-regulating the expression of bcl-2 and up-regulating that of bax and caspase-3.

Key words: Oridonin;Human glioma U251 cells; Apoptosis; Gene,bax; Gene,bcl-2;Gene,caspase-3

CLC Number: 

  • R651.1
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