Abstract:

Objective    To explore the effect of glutathione(GSH) combined with oxaliplatin(L-OHP) and cisplatin(CDDP) on the proliferation and apoptosis of gastric carcinoma cell line SGC-7901 cells.  Methods    The cells in five groups were treated according to the following drug administrations: GSH, CDDP, L-OHP, GSH combined with CDDP, and GSH combined with L-OHP. Cell proliferation was evaluated by SRB assay, inverted microscope and fluorescent microscope were used to observe morphological changes, and the distribution of cell cycle and the rate of apoptosis were observed by flow cytometery respectively. Results    GSH combined with L-OHP and CDDP obviously inhibited the proliferation of SGC-7901. The combination group of GSH and CDDP had no significant difference compared with the CDDP group (P>0.05), while the combination group of GSH and L-OHP had a significant difference compared with the L-OHP group (P<0.05). Whether cells were treated by single platinum derivative or by combination of GSH and platinum derivative, cancer cells showed the morphological change of apoptosis when observed microscopically. Flow cytometry analysis showed that the combination group of GSH and L-OHP and the L-OHP group had reduced number of cells in S phase and showed an apparent peak of apoptosis. However, the L-OHP group showed more obvious growth inhibition to SGC-7901 than the combination groups. Conclusion    GSH dose not reduce the effect of CDDP on inhibiting the growth of SGC-7901, but it reduces the effect of L-OHP on SGC-7901 cells, and reduces the apoptosis of gastric cancer cells.

Key words: Glutathione; Cisplatin; Oxaliplatin; Gastric cancer cell line; Cell proliferation; Apoptosis