Abstract:

Objective    To establish a high-performance liquid chromatography (HPLC) fingerprint of Shandong trueborn Pinellia ternata (Thunb.) Breit. for quality control.  Methods    HPLC was used by ODS column(4.6mm×250mm, 5μm) with the gradient elution solvert system composed of acetonitrile-water. The flow rate was 1.0mL/min, and the detection wavelength was 260nm. The HPLC fingerprints were compared by the similarity evaluation software and SPSS software.  Results    The HPLC fingerprint of Shandong trueborn Pinellia was established, and methods established for Pinellias conformed to the technical standards in fingerprint spectrum.  All the samples from Shandong Province were similar and were clustered into one group, and they were different from the commodity pinellia.  Conclusion    The established HPLC fingerprint has desirable accuracy, repeatability and stability. It can be used for the quality control of Pinellia ternata (Thunb.) Breit.       

Key words: Pinellia ternata (Thunb.) Breit.; Stratography, High-performance liquid chromatography; Similarity evaluation; SPSS statistical analysis