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Table of Content

      
    16 September 2010
    Volume 48 Issue 9
    Articles
    Effect of aldosterone on PTEN expression and the PI3K-Akt pathway in 3T3-L1 adipocytes
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  1. 
    Abstract ( 1489 )   PDF (939KB) ( 506 )   Save
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    Objective    To investigate the effects of aldosterone  on the expression of phosphoserine 473-Akt and PTEN in normal or insulin resistant adipocytes,  with the aim to explore the role of  aldosterone in the pathogenesis of insulin resistance. Methods    Insulin resistant 3T3-L1 adipocytes were induced by dexamethasone and insulin. The protein expressions of  PTEN and phosphoserine 473-AKT in normal and insulin resistant adipocytes treated with aldosterone or  spironolactone were measured by Western blot. Results    Compared with normal adipocytes, expression of PTEN protein increased(0.83±0.11)vs(0.63±0.06), P<0.05] and phosphoserine 473-AKT protein  decreased(0.52±0.12)vs(0.78±0.12), P<0.05] in insulin resistant models. Aldosterone-induced upregulation of PTEN and degradation of phosphoserine 473AKT was markedly attenuated by spironolactone,  a mineralocorticoid receptor antagonist(all P<0.05). Conclusion    Aldosterone induces insulin resistance possibly via the PI3K-Akt pathway in 3T3-L1 adipocytes.

    Contribution of overweight and obesity to the prevalence rate of diabetes and its other risk factors
    LI Peng-fei1, LOU Neng-jun2, LIU Yun-tai3,XIN Ying1, LIAO Lin1, DONG Jian-jun4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  5-8. 
    Abstract ( 1245 )   PDF (376KB) ( 281 )   Save
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    Objective    To investigate the contributions of overweight and obesity to the prevalence rate of diabetes and its other risk factors on the basis of a questionnaire of the diabetes epidemic survey model. Methods    Based on a questionnaire of epidemiological survey, obese and non-obese target populations were enrolled. According to the body mass index(BMI), the participants were divided into 2 groups: group A (BMI≥24kg/m2) and group B (BMI <24kg/m2). The prevalence rate of diabetes and impaired glucose tolerance(IGT) were calculated. Logistic regression analysis was carried out to screen the primary risk factors of diabetes. The score of the variables was determined by β-coefficients, and used to predict the risk of correlation factors. Results    The prevalence rate of diabetes and IGT were 6.7% and 17.5% in group A, and  1.1% and 7.0% in group B, respectively.  Age, family history of diabetes, waist-hip ratio (WHR), systolic pressure,  pulse, and history of high glucose were singled out as risk factors  in group A. Age, diastolicpressure,family history of diabetes, and history of high glucose were risk factors in group B.  A trend χ2 test yielded a statistical significance (P<0.001). Conclusion    The prevalence rate of diabetes and IGT were much higher in the overweight and obese population than in the nonobese population. Lifestyle modification can control this factor, it is promising to prevent or delay the development of diabetes mellitus and IGT. Age, blood pressure, family history of diabetes, and history of high blood sugar are all independent risk factors for diabetes.

    Epidemiological characteristics of overweight and obesity among adults of the Shandong  coastal area in 2004 
    SUN Rui-xia, CAO cai-xia, Li li, XIAN yu-xin, L wen-shan, WANG jing, GAO Yan-yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  9-13. 
    Abstract ( 1321 )   PDF (374KB) ( 319 )   Save
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    Objective    To investigate epidemiological characteristics of overweight and obesity among adults of the coastal area in Shandong Province in 2004, and to identify populations and  regions of overweight and obesity with high risk. Method    A random stratified cluster sampling was conducted in 5 coastal cities(Yantai, Weihai, Rizhao, Dongying, Qingdao). Data of general conditions were collected by face-to-face interview. Height and weight was obtained by physical examination. Prevalence and influencing factors of overweight and obesity were analyzed. Results    4752 participants were recruited . Mean body mass index(BMI) of the total population was(24.12±3.57)kg/m2 and no significant difference was found between males and females(24.22±3.43 vs 24.07±3.69, P>0.05), or cities and rural areas(24.22±3.56 vs 24.06±3.57, P>0.05). Mean waist circumference(WC) was(84.09±11.01)cm and a significant difference was observed between males and females(86.69±10.36 vs 81.70±11.06, P<0.01), but not between cities and rural areas (P>0.05). The standardized prevalence rates of overweight, obesity and abdominal obesity were 32.2%、13.3%、54.9%, respectively. The prevalence rates of overweight were higher in males than that in females (35.6% vs 30.8%,P<0.01), and in cities than that in rural areas(35.8% vs 32.4%,P<0.01). No significant differences were demonstrated between males and females, cities and rural areas in obesity and prevalence rates ofResults of logistic analysis showed the major factors influencing overweight included age, gender, location of residence,smoking/drinking habits, labor intensity, and physical exercises. Age, drinking habits, labor intensity, physical exercises were influencing factors of abdominal obesity. Conclusion    About half of the  residents of coastal areas in Shandong Province are overweight or obese, therefore, early interventions are desirable.

    Emodin ameliorates high-glucose-induced mesangial cell hypocontractility
    LIU You-xia1, LIU Yi2, CAO Ming-feng1, WAN Qiang1, WANG Qun1, WANG Rong1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  14-18. 
    Abstract ( 1263 )   PDF (1024KB) ( 420 )   Save
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    Objective    To explore whether emodin ameliorates high-glucose-induced mesangial cell hypo-contractility by inhibiting p38MAPK over-activation.  Methods    Mesangial cells were divided into five groups: the normal glucose group (5.6mmol/L glucose, NG group), the high glucose group (30mmol/L glucose, HG group), the low-dose emodin group (50mg/L emodin+ 30mmol/L glucose, LE group), the high-dose emodin group (100mg/L emodin+30mmol/L glucose, HE group), and the GW9662 group (10μmol/L GW9662+ 30mmol/L glucose+100mg/L emodin, GW group). Angiotensin Ⅱ-stimulated cell surface reduction was measured to evaluate cell contractility. p38MAPK activity and PPARγ expression were detected by using Western blot and real-time polymerase chain reaction(Real-time PCR).  Results    High-glucose resulted in an increase   in p38MAPK activity and significant impairment of mesangial contractility(P<0.05); emodin treatment dose-dependently inhibited high-glucose-induced p38MAPK overactivation(a 40% decrease for 50mg/L emodin and a 73% decrease for 100 mg/L emodin), and mesangial hypo-contractility was ameliorated by emodin(P<0.05); both the PPARγ mRNA and protein levels were elevated after emodin treatment(P<0.05);  inhibition of PPARγ using GW9662 effectively blocked ameliorating effects of emodin on high-glucose-induced p38MAPK over-activation and mesangial hypo-contractility(P<0.05). Conclusion     Emodin effectively ameliorates high-glucose-induced p38MAPK over- activation via activation of PPARγ, and therefore, ameliorateshypo-contractility of mesangial cells.

    eNOS gene transfection on pulmonary artery hypertensionand hypertension crisis in rabbits
    ZHANG Feng-wei1, WU Shu-ming2, CAO Guang-qing2, LU Xian-shuo2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  19-24. 
    Abstract ( 1324 )   PDF (678KB) ( 301 )   Save
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    Objective     Effects of transfection of the endothelial nitric oxide synthase (eNOS) gene were studied in rabbits with pulmonary hypertension  induced by left to right shunt or pulmonary hypertension crisis  induced by hypoxia . Methods    Eighteen pulmonary hypertension rabbits were divided into the experimental group(n=9) and the control group(n=9).In the experimental group,an adenoviral vector encoding eNOS was intratracheally transfected .The same dosage of saline was intratracheally transfected in the control group. The leftright shunt was ligated 4 days after transfection, while 10% oxygen was inhaled through endotracheal intubation. Changes of pulmonary artery systolic pressure (SPAP),mean pulmonary artery pressure(MPAP) and mean arterial pressure(MAP) were detected. Concentration of NO and transgene expression mRNA were investigated.  Results     Compared with that before transfection, pulmonary artery pressure  significantly decreased(P<0.01) in the experimental group, while there was no change in the control group. There were significant differences between two groups after transfection. 60min after hypoxia, all rabbits presented pulmonary hypertension crisis in the control group, but only 4 rabbits in the experimental group developed a crisis. Decreased PaO2 and MAP, increased PaCO2, SPAP and MPAP occurred in these two groups after hypoxia. But after the oxygen supply was restored, the variables recovered to normal levels in the experimental group. The variables of SPAP and MPAP were higher than their baseline, and MAP was lower in the control group. Comparing the variables (after  vs before hypoxia) between the control group and the experimental group, the increasing extent of SPAP,MPAP and PaCO2 and decreasing extent of MAP, CO and PaO2 in the experimental group were lower than those in the control group . With immunohistochemical staining,  expression of  the eNOS gene in alveolar endothelial cells, smooth muscle cells and endothelial cells of small pulmonary vessels of the experimental group increased more than that of the control group. The concentration of NO in the experimental group was higher than that in the control group. Agarose gel electrophoresis showed an amplified 3.77kb specific band in the experimental group, but not in the  control group. Conclusions    Intratracheal adenoviral-mediated eNOS gene transfection can reduce rabbits′ pulmonary hypertension induced by left-to-right shunt and decrease occurrence rate of pulmonary hypertension crisis.

    Relationship between anti-implantation effect of arnebia root and expression of muc1 in the endometrium of early pregnant mice
    REN Shao-da1, YU Hui2, MA Bao-hua1, LI Li-li1, HAO Ai-jun1, WANG Zhe-min3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  25-28. 
    Abstract ( 1482 )   PDF (1117KB) ( 377 )   Save
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    Objective     To explore the relationship between anti-implantation effect of arnebia root and expression of the muc1 protein in the endometrium of early pregnant mice.  Methods    Mated female mice were randomly divided into the experimental groups and the control group. The experimental groups were fed with an arnebia root solution at different concentrations and the control group was fed with saline on day 1,2, 3, 4 of pregnancy. The uteri of the mice were removed at night on day 4 of pregnancy. In order to observe the morphological changes and expression of the muc1 protein in the endometrium, one side of the uteri were stained with HE and immunohistochemistry. Expression of the muc1 protein on the other side of the uteri was measured by Western blot. The mated mice were executed to assess the implantation rate on day 8 of pregnancy. Results     The endometrium in the experimental groups exhibited morphological changes compared with that in the control group. Expression of the muc1 protein was positively related to the doses of arnebia root, and the difference between experimental groups and the control group was significant(P<0.05).The rates of embryo implantation in the experimental groups and the control group were significantly different(P<0.05). Conclusion    The arnebia root can prevent embryo implantation by inhibiting disappearance of the muc1 protein.

    Construction and verification of a recombinant plasmid encoding HBV surface genes S and PreS2 as a genetic adjuvant
    ZHAO Qun-li, MIN Juan, ZHOU Huai-yu, GU Qin-min, CONG Hua, LI Ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  29. 
    Abstract ( 1373 )   PDF (1117KB) ( 444 )   Save
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    Objective     To construct and verify a recombinant eukaryotic expression plasmid pVAX1-SPreS2 for the further development of a new genetic adjuvant to enhance or adjust humoral and cellular immune responses to a Toxoplasma gondii DNA vaccine. Methods    The S and PreS2 genes from HBV were fused with a polypeptide linker (GSGSGS) by overlapping extension PCR, constructing the cloning plasmid pMD18T-SPreS2 and recombinant eukaryotic expression plasmid pVAX1-SPreS2. Then the construction was verified by PCR, restriction enzyme digestion and sequencing. The specific expression protein SPreS2 was detected by SDS-PAGE and Western blot after transient transfection of pVAX1-SPreS2 into HFF cells, which were treated with lipofectin to induce the establishment of an in vitro eukaryotic cell transient expression system. Results    PCR, restriction enzyme digestion and sequencing results showed the correct construction of the recombinant cloning plasmid pMD18T-SPreS2 and eukaryotic expression plasmid pVAX1-SPreS2. It was found that the fusion gene SPreS2 was transiently expressed in eukaryotic cells and its product had, to some extent, an immunological activity. Conclusion     The recombinant eukaryotic expression plasmid pVAX1-SPreS2 has been successfully constructed and it may function as a new genetic adjuvant for a T. gondii DNA vaccine.

    Construction and expression of a prokaryotic vector encoding outer membrane protein OprF of Pseudomonas aeruginosa
    WANG Yan1, DOU Heng-li2, HU Cheng-jin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  34-39. 
    Abstract ( 1659 )   PDF (842KB) ( 892 )   Save
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    Objective     To clone the outer membrane protein OprF of Pseudomonas aeruginosa(PA), and to construct and identify a prokaryotic expression plasmid. Methods    The DNA genome was extracted from PA, and the carboxyl terminus of the OprF gene was amplified by primers of PCR. The recombinant plasmid pET28b-F was constructed by cloning the OprF cDNA into the prokaryotic expression vector pET28b. After being identified by restriction endonuclease digestion analysis and DNA sequencing, the pET28b-F was transformed into E. coli BL21 and induced by IPTG. The expressed protein was analyzed by SDS-PAGE and Western blot. Monoclonal antibodies(McAbs) against OprF were prepared by the hybidoma technique and screened by ELISA. Results    The construction of the recombinant expression plasmid pET28b-F was correct by restriction enzyme, PCR and DNA sequencing. Then the expression plasmid expressed a corresponding protein OprF after induction of IPTG. Four McAbs could specifically combine with the OprF protein of PA. Conclusion    Successful construction of OprF in the prokaryotic expression vector and specific McAbs established with this method can provide the basis for further research into preparing the specific antibody to OprF and into conveniently detecting PA in wound infection.

    Effect of tacrolimus (FK506) on apoptosis cells and MMP and AIF expressions in rats with epilepsy
    XUE Ping,LIU Jin-zhi, HUANG Yan-fei,WANG Ai-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  40-43. 
    Abstract ( 1234 )   PDF (1078KB) ( 526 )   Save
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    Objective    To observe the effect of immunosuppressant tacrolimus (FK506) on neuronal apoptosis in the hippocampus, mitochondrial membrane potential (MMP) and apoptosis-inducing factor (AIF) expression so as to provide a theoretical basis of cerebral protection after epilepsy. Methods    108 healthy adult male Wistar rats were randomly divided into the epilepsy group, the tacrolimus group and the control group. The epilepsy group and the tacrolimus group were intraperitoneally injected with pilocarpine to establish status epilepitcus models.The tacrolimus guoup was pre-treated with tacrolimus 24h and 1h before pilocarpine injection, while the control group only received an injection of an equal amount of saline. The number of apoptotic cells was detected by TUNEL technique, fluorescence intensity of rhodamine 123 and size of mitochondria by flow cytometry, and expression of AIF by immunohistochemical analysis. Results    Compared with the tacrolimus group and the control group, the number of apoptotic cells and expression of AIF in the epilepsy group were higher and the mitochondrial membrane potential was increased(P<0.05),and the cerebral protection group and the control group had no significant difference(P>0.05).Conclusion    FK506 plays an important role in neuro-protection by reducing neuronal apoptosis and inhibiting MMP changes.

    Influence of hypoxia on expressions of relative factors of the Wnt/β-catenin signal pathway in neural stem cells in vitro
    CUI Xiao-ping1, LIN Hang1, CHEN Jian-mei2, MU Jun-shan1, YE Jian-xin1, YIN Hong-bing1, YING Da-jun3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  44-48. 
    Abstract ( 1324 )   PDF (919KB) ( 611 )   Save
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     Objective    To observe expressions of relative factors of the Wnt/β-catenin signal pathway in neural stem cells (NSCs) cultured in vitro in normoxia(20%O2) and hypoxia (3±2%O2) and explore the mechanism of proliferation of NSCs in hypoxia. Methods    NSCs obtained from the hippocampus of a neonatal mouse were cultured in normoxia and hypoxia, then expressions of major factors of the Wnt/β-catenin pathway were detected by RT-PCR. After the luciferase reporter gene plasmid was transfected into NSCs by electroporation, responsive ability of the Wnt/β-catenin pathway to hypoxia was detected, and expressions of β-catenin and CyclinD1 in NSCs were detected by Western blot.  Results    The major factors in the Wnt/β-catenin signal pathway were detected in NSCs and the Wnt/β-catenin signal pathway was obviously responsive to hypoxia. The β-catenin and CyclinD1 protein levels were increased after hypoxia treatment compared with those in normoxia(P<0.05). Conclusion    The Wnt/β-catenin signal pathway may promote the proliferation of NSCs in hypoxia by increasing expressions of β-catenin and CyclinD1 in vitro.

    Protective effect of Naoxintong on reperfusion after focal cerebral ischemia in rats
    QU Li-xin1,2, SU Shao-hua2, YANG Li-ling1, LI Jia-long1, REN Xiao-yan1, DU Yi-feng1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  49. 
    Abstract ( 1375 )   PDF (1170KB) ( 458 )   Save
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    Objective     To investigate the protective effect of Naoxintong on reperfusion after focal cerebral ischemia in rats and explore effective methods to interfere with inflammation following acute cerebral ischemia-reperfusion injury. Methods     Thirty male SD rats were randomly divided into three groups: the sham operation group(group A, n=10), the ischemic group (group B, n=10), and the Naoxintong group(group C, n=10). Group B and C had focal cerebral ischemia-reperfusion produced by right middle cerebral artery occlusion. Activation of microglia, and expressions of CD68,IL-1β and TNF-α were detected by immunohistochemistry and Westernblot. Results    Expressions of CD68, IL-1β and TNF-α were more markedly increased in group B than those in group A, and significantly lower in group C than those in group B. Conclusion    Naoxintong can inhibit inflammation reaction after cerebral ischemia, which can provide an inhibitory effect on cerebral ischemiareperfusion injury in rats.

    Differential expressions of the mitochondrial proteome in the hippocampus of pharmaco-resistant epileptic rats
    JIANG Wen-jing1, DU Bin-feng2, CHI Zhao-fu3, MA Lin1, SHAN Pei-yan1, ZHANG Xu-hua4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  53-58. 
    Abstract ( 1191 )   PDF (781KB) ( 630 )   Save
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    Objective    To explore the pathogenesis mechanism and the potential therapeutic targets of intractable epilepsy through the technique of mitochondrial proteomics in pharmacoresistant epileptic models. Methods    Expression profiles of mitochondrial proteins in pharmacoresistant rats and non-pharmaco-resistant rats were compared and analyzed by using immobilized pH gradient, SDS-PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Results    All 19 protein spots were differentially expressed when compared with non-pharmaco-resistant epileptic rats. 5 up-regulated proteins in pharmaco-resistant epileptic hippocampal mitochondria were glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase-1, voltage-dependent anion channel 1, aldose A, and tubulin beta 2. 14 down-regulated proteins in pharmacoresistant epileptic hippocampal mitochondria were aconitase, glutamate dehydrogenase 1, malate dehydrogenase, NADH dehydrogenase 1 alpha subcomplex 10, ATP synthase alpha subunit precursor, similar to pyruvate kinase isoenzyme, ATP binding cassette, subfamily B, member 10, phospholipase A2, serine proteinase inhibitor, serpin, heat shock protein 70, uncoupling protein, cofilin 1, aldehyde dehydrogenase, and voltage-dependent anion channel 2. Conclusion    Many mitochondrial proteins are differentially expressed, which are perhaps involved in the pathogenesis of intractable epilepsy.

    Effects of the plateletderived growth factor on proliferation and extracellularmatrix of human umbilical cord mesenchymal stem cells
    ZHUANG Yong1, LI Dong2, SHI Qing2, WANG Da-kun2, CAI Zhi-feng1, JU Xiu-li1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  59-63. 
    Abstract ( 1335 )   PDF (1239KB) ( 387 )   Save
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    Objective    To investigate the effects of the platelet-derived growth factor(PDGF) on the proliferation, extracellular matrix  synthesis and its probable mechanism in human umbilical cord mesenchymal stem cells(UCMSCs). Methods    Cultured UCMSCs were divided into 6 groups: the control group, and the PDGF-treated groups(2, 5, 10, 20 and 50μg/L).  Morphological changes of the cells were observed under an inverted microscope.  MTT assay was used to detect the PDGF effects on cell proliferation.  Expressions of ECM mRNA and apoptosis-related genes(Bcl-2, Bax)were detected by qRT-PCR. Results    With an increased concentration of PDGF, UCMSCs proliferation was gradually promoted compared with the control group. 20μg/L PDGF greatly promoted UCMSCs proliferation, however, 50μg/L showed  inhibitive effects. Expressions of ECM mRNA and apoptosis-related factor (Bcl-2/Bax) were down-regulated in the 2μg/L PDGF group but up-regulated when PDGF≥5μg/L. 10μg/L PDGF greatly upregulated ECM mRNA expression and apoptosis-related factor (Bcl2/Bax). Conclusion    PDGF can promote the proliferation of UCMSCs, stimulate ECM synthesis and improve the ability to withstand apoptosis of UCMSCs in vitro. 10μg/L PDGF was the most effective concentration.

     Effects of low-dose ketamine administration on behavior and ultrastructures of   the hippocampus and prefrontal cortex in Cynomolgus monkeys
    WANG Li1, JIANG Hong1, YU Hong-luan2, SUN Lin1, PAN Fang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  64. 
    Abstract ( 1333 )   PDF (1518KB) ( 478 )   Save
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    Objective    To explore effects of long-term low-dose ketamine on behavior and ultrastructures of the hippocampus and prefrontal cortex in Cynomolgus monkeys.  Methods    12 male Cynomolgus monkeys were randomly divided into the ketamine group (n=8) and the control group (n=4). The ketamine group was daily intravenously injected with ketamine(1mg/kg), while the control group injected with normal saline(1mL/kg).  Observations on behavior were regularly made and ultrastructural changes of the hippocampus and prefrontal cortex were observed by a transmission electron microscope after 6 months. Results    There was no statistically significant difference in locomotor activities between the two groups(P﹥0.05).Ultrastructural changes in the ketamine group, including neuron necrosis, glial cell apoptosis and lipofuscin accumulation, appeared in the hippocampus; a mass of autophagosomes, reduction of the rough endoplasmic reticulum, neuron necrosis, and glial cell apoptosis were observed in the prefrontal cortex in the ketamine group, while not observed in the control group. Conclusion     Long-term low-dose ketamine induces ultrastructural changes in the hippocampus and prefrontal cortex, including neuron degeneration, necrosis and apoptosis, which appear earlier than behavioral changes do.

    Effects of different preparation techniques on the sealing of root fillings in curved teeth in vitro
    LIU Zhi-yong1, SHENG Fang1, HAO Xin-yu1, XIONG Shi-jiang1,2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  68-71. 
    Abstract ( 1296 )   PDF (369KB) ( 603 )   Save
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    Objective     To evaluate the sealing ability of curved root canals prepared by different preparation methods. Methods     Sixty curved human maxillary anterior and premolar teeth were divided randomly into four experimental groups (A,B,C,D; 12 teeth per group)and two control groups(E,F; 6 teeth per group). The experimental groups were prepared by using the balanced force technique(A), the ProTaper/crown-down technique(B), the step-down technique(C), the step-back technique(D) and obturated by cold laterally compacted gutta-percha with Cortisomol sealer. Control groups included six positive and six negative subjects. Leakage along the root filling was measured by concentration of glucose in the apical reservoir on the thirtieth days with the O-TB method. Results     Leakage was outstanding in the positive controls but could not be detected in the negative controls. Mean concentrations of glucose of the four experimental groups were (4.22±0.53), (4.51±0.70), (4.86±1.30) and (7.62±1.35)mmol/L, respectively. There was significant difference between the step-back technique and the other three preparation methods (P<0.05). No difference among the balanced force technique group, the ProTaper/crown-down technique and step-down technique group was observed (P>0.05). Conclusion     The sealing ability of the balanced force technique, the ProTaper/crown-down technique and the step-down technique is better than that of  the step-back technique in curved teeth in vitro.

    Clinical research of risk factors related to sexual activity  after myocardial infarction
    XING Shan-shan1, ZHANG Wei2, XING Qi-chong3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  72-75. 
    Abstract ( 1363 )   PDF (357KB) ( 554 )   Save
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    Objective    To investigate whether sexual activity after myocardial infarction can trigger a new myocardial ischemia or injury. Methods     A total of 32 men experiencing first myocardial infarction were examined. All subjects wore a Holter ECG during both sexual activity and physical exercise. Heart rate variability (HRV) and SDNN, SDANNI, RMSSD, LF,  HF were quantitatively analyzed. Results    The index of SDNN, SDANNI, RMSSD, LF, HF were 95.3ms, 76.5ms, 18.3ms, 738.5ms2, 520.7ms2 respectively during sexual activity; and 114.5ms, 87.4ms, 25.4ms, 583.7ms2, 548.5ms2 respectively during physical exercise. The differences were significant (P<0.01). Conclusion    Sexual activity might induce myocardial ischemia/injury or arrhythmia. Quantitative analysis of HRV can reflect vagal tone and sympathetic nerve activity, and therefore, it may guide safe sexual activity after myocardial infarction.

    Analysis of visual performance corrected by rigid gas permeable contact lens in keratoconus
    WEN Ying1,2, WU Xin-yi1, JI Peng2, WANG Gui-min2, LI Hai-yan2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  76. 
    Abstract ( 1458 )   PDF (541KB) ( 327 )   Save
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    Objective     To analyze visual performance in keratoconus with rigid gas permeable contact lens (RGPCL). Methods      18 keratoconic patients (36 eyes) who were fitted with RGPCL were enrolled as keratoconus group. 9 myopic patients(18 eyes) with RGPCL were enrolled as the myopia group. Visual acuity(VA) and contrast sensitivity were examined to evaluate visual performance(VP). Corneal astigmatism and wavefront error were measured to analyze the influencing factors of VP. Results    All keratoconic eyes got corrected VA of 0.6 or better.61.11% of them reached 1.0. There was no difference between the two groups in low frequency of contrast sensitivity(P>0.05).But in the middle frequency of light-off contrast sensitivity and in the high frequency of contrast sensitivity,the keratoconus group was significantly lower than the myopia group(P<0.05). Corneal astigmatism was reduced from (8.25±3.61)D to (1.25±0.13)D in the keratoconus group and from(1.28±0.34)D to (0.42±0.06)D in the myopia group. The root-meansquare of total higher-order aberrations (HOAs), coma-like aberration and spherical-like aberrations in the keratoconus group were significantly higher than those in the myopia group(P<0.05). Conclusion     Through RGPCL, keratoconic eyes can get corrected VA as well as myopic eyes with RGPCL, but can not get ideal visual performance. The degradation of visual performance may be related to residual corneal astigmatism and HOAs in keratoconic eyes with RGPCL.

    Value of SP-D levels in the sputum in the diagnosis of pulmonary tuberculosis
    HU Hua, XU Yong, CHI Jingyu, JU Yunfei, YANG Yan, HOU Bao-qing, LI Xue-zheng, MENG Fan-min, LI Lei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  79-81. 
    Abstract ( 1324 )   PDF (313KB) ( 367 )   Save
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    Objective    To observe the level of surfactant protein-D(SP-D) in the sputum in pulmonary tuberculosis patients, and explore its value in the diagnosis of pulmonary tuberculosis. Methods     The SP-D level in the sputum was detected by ELISA in 100 samples from pulmonary tuberculosis patients and 84 samples from healthy subjects. The leucocyte count, neutrophilic granulocyte percentage and lymphocyte percentage were determined with the electric resistance method, erythrocyte sedimentation rates by the sedimentation process, and tubercle bacillus antibodies by ELISA. The PPD trial was executed with the Mantoux method. Results     Level of SP-D in the sputum in pulmonary tuberculosis was much lower than that in healthy subjects(P<0.05), and there was no obvious difference between pulmonary tuberculosis patients with different genders, ages and other factors (P>0.05). Conclusions     Detection of SP-D in the sputum is of great value in the diagnosis and differential diagnosis of pulmonary tuberculosis. The gender, age and other factors have nothing to do with the level of SP-D in the sputum in pulmonary tuberculosis.

    Complicated parapneumonic effusions treated by medical thoracoscopy
    DU Li-ming, ZHOU Zhen, WANG Zeng, XIE De-rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  82. 
    Abstract ( 1496 )   PDF (371KB) ( 394 )   Save
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    Objective     To determine the optimal treatment of complicated parapneumonic effusions. Methods     Twenty-one patients with confirmed parapneumonic effusions were randomly divided to receive either chest tube pleural drainage plus streptokinase (CTSKs, n=10) or medical thoracoscopy (MTs, n=11). Outcome analysis with respect to treatment efficacy, hospital duration, chest tube duration, hospital costs, and need for subsequent procedures was performed.  Results    Compared with the CT-SK group, the MT group had a significantly higher primary treatment success [10/11, 91% vs 4/9, 40%; P<0.05 Fisher′s Exact Test], lower chest tube duration (5.8±1.1 vs 9.8±1.3 days; P<0.03), shorter total hospital days (8.7±0.9 vs 12.8±1.1 days; P<0.05), and less hospital costs (11045±3411 vs  18475 ±5164, P<0.05) also favored the MTs group. Of note, all the CT-SKs treatment failures could be salvaged with MTs, and none required thoracotomy.  Conclusion     In patients with complicated parapneumonic effusions and thoracic empyema, a primary treatment strategy of MTs is associated with a higher efficacy, shorter hospital duration, and less cost than a treatment strategy that utilizes catheterdirected fibrinolytic therapy.

    Clinical effects of Biapenem in patients with acute exacerbation ofchronic obstructive pulmonary disease
    WU Yan, HUI Fu-xin, FAN Xiao-dong, WANG Jia-kun, SUN Hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  85-88. 
    Abstract ( 1456 )   PDF (355KB) ( 391 )   Save
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    Objective    To observe the efficacy and safety of Biapenem in the treatment of Anthonisen I and mechanical ventilation will be demanded in acute exacerbation of chronic obstructive pulmonary disease(AECOPD). Methods     A control study of Biapenem (the treatment group) and imipenem-cilastatin (the control group) in the treatment of 68 patients was carried out.The C-reactive protein levels were measured on admission, and on day 3 patients with a decline of <50% in CRP levels in the two groups were compared. Results    The total effective rates of Biapenem and imipenem-cilastatin were 90.6.3% and 86.1% and bacterial clearance rates were 90.3 and 78.3%, respectively. An obvious decrease of CRP level was found in 3 days, but patients with a decline of <50% in CRP had no obvious difference between the two groups. Serious adverse effect was not seen. Conclusion     Biapenem is effective and safe in treating patients with Anthonisen I and mechanical ventilation will be demanded in AECOPD and has good bacterial clearance rates.

    Peripheral  expressions CD81 and CD4 in chronic HCV  patients
    CHENG Guang-xia1,2, ZHANG Ling1, LIU Jing-wen2, FENG Zhao-lei2, LI Cui-ling1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  89. 
    Abstract ( 1284 )   PDF (1013KB) ( 376 )   Save
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     Objective     To investigate peripheral CD81 and CD4 expressions in patients infected by chronic Hepatitis C Virus (HCV),  and explore the modulation effect of CD81 on the immune system. Methods      Samples were collected from 55 chronic hepatitis C patients (infection group) and 25 healthy persons (control group). Peripheral expression of CD81 and CD4 were measured by flow cytometry. Serum level of IL2 and  HCV RNA were detected by ELISA and RTPCR, respectively. Results    The peripheral level of  CD81+ cells and the serum level of HCV RNA were positively correlated(r=0.474, P<0.01) in the infection group. The numbers of the peripheral CD81+  lymphocytes and the CD3+CD4+ CD81+T cells among CD3+CD4+ T cells in the infection group were higher than those in the control group (P<0.01). Both were positively correlated(r=0.581,P<0.01). Also, the number of peripheral CD3+CD4+T cells and the serum level of IL2 in the infection group were considerably lower than those in the control group (P<0.05). Both were also positively correlated(r=0.378, P<0.05). Conclusion    After HCV infection, the consistent presence of HCV RNA can promote expression of CD81 while number of CD3+CD4+T cells and secretion of  IL-2 decline, which participate in the immuno-regulation of the patients.

    Fibroscan in the diagnosis of hepatitis B related fibrosis
    SHAN Rong1, WANG Ai-guang2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  93-96. 
    Abstract ( 1533 )   PDF (480KB) ( 514 )   Save
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    Objective     To investigate the clinical value of fibroscan (FS) in the diagnosis of hepatitis B related fibrosis. Methods     Stiffness, hyaluronic acid(HA), laminin(LN), propeptide type Ⅲ of procollagen (PⅢP) and collagen Type Ⅳ(CV-IV) were detected in 59 chronic hepatitis B patients having undergone liver biopsy. The diagnostic values of FS and serum liver fibrosis markers were tested by the receiver operating characteristic (ROC) curve, using liver biopsy as the  gold standard. Results    In patients with moderate or serious fibrosis, the stiffness value was significantly high, and correlated with the fibrosis degree (r=0.76, P=0.00).The ROC curve analysis showed that the areas under the curve for stiffness, HA, LN, PⅢP and CV-IV were 0.98, 0.82, 0.74, 0.59 and 0.76 respectively. The sensitivity and specificity for  stiffness were higher than those with serum liver fibrosis markers. Conclusion    FS is an effective method in the diagnosis of hepatitis B related fibrosis.

    Preliminary application of Montreal cognitive assessment in vascular cognitive impairment
    WANG De-wei, XIE Zhao-hong, LAI Chao, ZHU Zheng-yu, WANG Yun, JIN Su-qin, BI Jian-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  97. 
    Abstract ( 1657 )   PDF (330KB) ( 540 )   Save
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     Objective     To study the application of Montreal cognitive assessment (MoCA) in vascular cognitive impairment (VCI), and make a benchmark for a cutoff score. Methods   60 healthy elderly controls and 50 patients meeting the clinical criteria for VCI were tested by MoCA and mini-mental state examination(MMSE). The validities of MoCA and MMSE were compared. The age, sex, education and nature of the work in the two groups were stratified and analyzed. Results    Education and nature of the work had statistical differences between MoCA and MMSE. In persons whose education level was below junior high school, the cutoff score for MoCA was ≥22, and the sensitivity was 83.3%, which was higher than that for MMSE sensitivity (46.7%); in those whose education level was above junior high school, the cutoff score for MoCA was ≥25, the sensitivity was 85%, which was higher than that for MMSE (50%). Conclusion     MoCA has a higher screening sensitivity for VCI than MMSE do. Adjustment in the cutoff scores would improve the detection of VCI by reducing false negativity. MoCA scores should be used to identify current cognitive difficulties but not be used to make formal diagnosis.

    Expressions of estrogen receptor, progesterone receptor and c-erbB-2and their relationship with prognosis in breast carcinoma tissues
    XU Yue-juan, QIU Shao-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  100-103. 
    Abstract ( 1522 )   PDF (540KB) ( 375 )   Save
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    Objective     To study expressions of estrogen receptor(ER),progesterone receptor(PR) and c-erbB-2and to explore their relationship with prognosis in breast carcinoma tissues. Methods    The immunohistochemical SP method was used to detect expressions of c-erbB-2, ER and PR from 193 breast carcinoma tissues. Results    Expression rate of c-erbB-2, ER and PR was 40.9%, 62.0% and 58.0%, respectively. Positive expression rate of c-erbB2 was correlated with tumor histological stage, clinical stage, and axillary lymph node metastasis. The total five-year survival rate was lower in the positive expression of cerbB2 group than in the negative group. Expression of ER had positive correlation with PR(P﹤0.01). The positive expression of c-erbB-2 had negative correlation with ER and PR(P﹤0.05).Conclusion     Detecting expressions of c-erbB-2, ER and PR gene in breast carcinoma tissues has an important role for leading clinical therapy and determining prognosis of breast carcinoma patients.

    Expression of BclXl and its relationship with clinical pathology in breast cancer tissues
    LIU Yun-cong1, RAN Li2, XIE Fei3, TAN Shi-sheng1, LI Hang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  104-106. 
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    Objective    To detect  apoptosis-suppressor gene Bcl-xl expression in breast cancer tissues, and to discuss its correlation with clinical pathology characteristics and estrogen receptor (ER) and  progesterone receptor (PR) expressions. Methods    Bcl-xl, ER and PR expressions were determined in 39 cases of breast cancer tissues, 39 cases of breast tissues adjacent to carcinoma, 30 cases of hyperplastic breast tissues and 30 cases of normal breast tissues by the S-P method. Results    Compared with  normal breast tissues adjacent to carcinoma and hyperplasia and normal breast tissues, the positive rate of Bcl-xl in breast cancer tissues was significantly higher than the former (P<0.05). There were relations between the clinical stage and expression of Bclxl, the higher the stage, the more the expression (P<0.05). The same situation was on classification of tumor cells. There were some negative correlations among expressions of ER, PR and Bcl-xl (P<0.05), also there was a relationship with axillary lymph node metastasis (P<0.05). Conclusion    The apoptosis suppressor gene Bcl-xl in breast cancer tissues had significant relation to clinical stage, classification of tumor cells, axillary lymph node metastasis or not and expressions of ER and PR, it may play an important epigenetics role in breast cancer.

    Evaluation of therapeutic efficiency of analgecine injection onpostherpetic neuralgia
    ZHANG Guo-zhuan1, LIANG Li-shuang1, YU Shao-hua2, LIU Xu-dong1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  107-109. 
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    Objective     To estimate the effectiveness of analgecine injection on postherpetic neuralgia(PHN). Methods     A total of 87 elderly outpatients suffering from PHN were enrolled. All patients aged more than 60 years were randomly divided into group A and group B: patients in group A were treated with conventional therapy, including analgesia, neurotrophic, and physical therapies,  whereas patients in group B were given additional 3.6IU of analgecine twice daily for 15 days. All patients were tested by VAS and QS when treated (T0) and followed-up for 7 days(T1),15days(T2),and 1 month(T3). Results     Scores of VAS and QS in group A and group B were significantly ameliorated compared with those before treatment (P<0.01).As time went on, there was a more significant amelioration in group B than that in group A. Compared with group A, there was no side effect related with analgecine injection in group B, such as hepatic lesion and anaphylactic reactions. Conclusion     Analgecine injection can rapidly and significantly alleviate the dispersion of pain and improve the QS of the patients with PHN. Analgecine injection is effective and safe in the treatment of PHN.

    Endovascular treatment for intracranial aneurysms associated with Moyamoya disease
    MENG Lei1, PANG Qi2, XU Shang-chen2, ZHAO Guang-yu2, DONG Ya-nan1, LUAN Li-ming2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  110-112. 
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    Objective     To explore endovascular treatment for intracranial aneurysms associated with Moyamoya disease. Methods    The clinical data of five cases of intracranial aneurysm associated with Moyamoya disease treated with embolization were retrospectively analyzed. Results      Four of  five cases presented subarachnoid hemorrhage and one intraventricular hemorrhage. Four cases belonged to the major artery type and one peripheral artery type. All of the aneurysms were successfully embolized. All patients had excellent clinical outcomes. Conclusion    Endovascular treatment is feasible for aneurysms associated with Moyamoya disease.

    Influencing factors on the traction plus epidural block for lumbar disc herniation
    LIU Xiu-hua1, GAO Jie2, YUE Shou-wei1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  113-116. 
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    Objective    To explore  related factors with the effect of epidural block plus SLR exercise for lumbar disc herniation, and further improve the efficacy of treatment. Methods    The data of 270 lumbar disc herniation cases, treated with epidural block plus SLR exercise from January 2005 to January 2009 at the Department of Rehabilitation Qilu Hospital of Shandong University,  were reviewed. Gender, age, incentives, occupation, duration, past medical history, clinical symptoms (JOA score),  protruding type, protruding segment, protruding size, and spinal canal or lateral recess stenosis were observed.  Which treatment efficacy was the dependent variable, while the 12 other factors were the variables. Single factor analysis was performed, and multivariate logistic regression analysis was be done on the significant factors singled out. Results    Univariate analysis showed that duration, place of stenosis (spinal canal or lateral recess), with or without predisposing factors, clinical symptoms (JOA score), protruding type, and protruding segment were significantly related to the prognosis of lumbar disc herniation(P<0.05). Multivariate logistic regression analysis further revealed that duration, place of stenosis, and predisposing factors were independent risk factors affecting the efficacy. Conclusion    Long duration, spinal canal and/or lateral recess stenosis, and no predisposing factors are independent risk factors which can affect the results of epidural block plus SLR exercise for lumbar disc herniation. These factors should be considered during the treatment.

    Analysis of nosocomial infection according to surveillance data of 38195 inpatients
    ZHANG Shui-hong1, WANG Lu2, LI Yu-zhu3, ZHANG Xia1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  117. 
    Abstract ( 1452 )   PDF (350KB) ( 413 )   Save
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     Objective    To study the status and development tendency of nosocomial infection in the patients of a provincial integrated hospital and to provide a basis for taking effective preventive and control measures. Methods    Everyday the nosocomial infection case report card was completed according to nosocomial infection cases screened by specialized staffs and the cases diagnosed by clinical physicians in different departments . The surveillance data were analyzed by software SPSS11.5. Results    A total of 38195 inpatients were screened from January 2008 to December 2009. Nosocomial infection was found in 630 inpatients (647 times), and the infection rate and infection times rate were 1.65% and 1.69% respectively. The upper respiratory tract was the most common infection site (38.79%) and ICU was the department with the highest infection rate (32.34%). Gram-negative bacteria were the majority of pathogens for nosocomial infections. The main pathogens included Pneumonia subspecies of Klebsiella pneumoniae, P seudomonas aeruginosa and Staphyloccocus aureus. Conclusions    During the surveillance period, the distribution and pathogens of nosocomial infection in this hospital were similar to the results of related reports. And there was no outbreak of severe nosocomial infection. Some measures should be taken to effectively reduce the occurrence of nosocomial infection.

     

    Review of nine cases of Xlinked agammaglobulinemia
    SUN Li-feng1, JU Yun-fei2, JIANG Li-ping3, WANG Jin-rong1, FENG Yi-zhen1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  120-123. 
    Abstract ( 1413 )   PDF (376KB) ( 352 )   Save
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    Objective    To explore clinical features, therapeutics and prognosis of X-linked agammaglobulinemia(XLA). Methods    9 confirmed XLA patients were analyzed by detecting the peripheral level of immunoglobulin and molecules on the cells′ surface. Clinical features were reviewed. BTK (Bruton′s tyrosine kinase ) gene  sequencing were performed in 2 cases. Results    Recurrent upper respiratory infections and pneumonia were found in all patients. Refractory otitis(3/9) and  purulent arthritis (4/9) were common. Skin infection(1/9), paralysis of legs(1/9), dilated cardiomyopathy(1/9), chronic diarrhea(1/9) and polyarthritis(1/9) appeared in different cases. All but one cases showed normal growth and development. The prominent signs at diagnosis were markedly decreased or absent tonsils and lymph nodes. Concentration of  serum immunoglobulins (Igs) and numbers of peripheral B cells in all cases were dramatically decreased. Two cases demonstrated mutations  of the BTK genes. Conclusion    XLA is preventable and curable. Standard treatment can yield good prognosis.

    Differentiation of HMSCs into hepatocyte-like cells in Fischer 344 rats in vivo
    CHEN Jian-feng1, GAO Yi2, GONG Lin1, PAN Ming-xin2, JIANG Ze-sheng2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  124. 
    Abstract ( 1265 )   PDF (775KB) ( 595 )   Save
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    Objective     To investigate the possibility of inducing human bone marrow mesenchymal stem cells(HMSCs) to differentiate into hepatocyte-like cells in liver injury rat models.   Methods    HMSCs were induced to differentiate into hepatocyte-like cells after being transplanted through the portal vein into the injured Fischer 344 rats which previously underwent  partial hepatectomy (PH) or whole body irradiation (IR). The hepatic metabolic functions  were measured by determining the alanime transaminase(ALT), total bilirubin(TBiL) and prothrombin time(PT) after model-building. Expression of the human specific DNA fragment Alu-sx was detected in the liver of Fischer 344 rats by PCR. Results   The hepatic metabolic functions of the rats in cell transplantation groups were apparently better than in the control group. Expressions of human specific DNA fragment Alu-sx and rat specific DNA fragment Sox11 were positive in the livers of cell transplantation groups. Conclusion    HMSCs can survive, differentiate into functional hepatocyte-like cells and express the human specific antigen in injured livers of Fischer 344 rats, contributing to the recovery of liver functions.

    Preoperative assessment of UPPP with a portable apneagraph     
    HUANG Xia-fei1, LIU Wei-zhi2, XIAO Kuan-lin3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2010, 48(9):  127-130. 
    Abstract ( 1880 )   PDF (352KB) ( 355 )   Save
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    Objective    To evaluate the clinical value of a portable apneagragh in the preoperative assessment of  uvulopalatopharyngoplasty(UPPP). Methods    Twenty-one patients with obstructive sleep apnea-hypopnea syndrome(OSAHS) were subjected to  UPPP by the Friedman staging system. Two days before UPPP, all  underwent a portable apneagraph study in the ward. Results   1.The apneagraph provided detailed parameters about sleep-related breathing disorders and the level of upper airway obstruction. 2.Most of the patients had both upper and lower obstruction. Sixteen cases(76.19%) showed predominantly upper pharyngeal obstruction and five cases(23.81%) showed predominantly lower pharyngeal obstruction. 3.There was little correlation between the retropalatal obstruction localized by the apneagraph and the Friedman′s stages. Conclusion    The portable apneagraph can analyze sleep-related breathing disorders and identify the level of upper airway obstruction. It has great directive significance in selecting patients for UPPP.