Abstract:

Objective    To observe the level of surfactant protein-D(SP-D) in the sputum in pulmonary tuberculosis patients, and explore its value in the diagnosis of pulmonary tuberculosis. Methods     The SP-D level in the sputum was detected by ELISA in 100 samples from pulmonary tuberculosis patients and 84 samples from healthy subjects. The leucocyte count, neutrophilic granulocyte percentage and lymphocyte percentage were determined with the electric resistance method, erythrocyte sedimentation rates by the sedimentation process, and tubercle bacillus antibodies by ELISA. The PPD trial was executed with the Mantoux method. Results     Level of SP-D in the sputum in pulmonary tuberculosis was much lower than that in healthy subjects(P<0.05), and there was no obvious difference between pulmonary tuberculosis patients with different genders, ages and other factors (P>0.05). Conclusions     Detection of SP-D in the sputum is of great value in the diagnosis and differential diagnosis of pulmonary tuberculosis. The gender, age and other factors have nothing to do with the level of SP-D in the sputum in pulmonary tuberculosis.

Key words: Tuberculosis, pulmonary；Sputum；Diagnosis；Surfactant protein-D