Abstract:

Objective     To investigate the protective effect of Naoxintong on reperfusion after focal cerebral ischemia in rats and explore effective methods to interfere with inflammation following acute cerebral ischemia-reperfusion injury. Methods     Thirty male SD rats were randomly divided into three groups: the sham operation group(group A, n=10), the ischemic group (group B, n=10), and the Naoxintong group(group C, n=10). Group B and C had focal cerebral ischemia-reperfusion produced by right middle cerebral artery occlusion. Activation of microglia， and expressions of CD68，IL-1β and TNF-α were detected by immunohistochemistry and Westernblot． Results    Expressions of CD68, IL-1β and TNF-α were more markedly increased in group B than those in group A, and significantly lower in group C than those in group B． Conclusion    Naoxintong can inhibit inflammation reaction after cerebral ischemia, which can provide an inhibitory effect on cerebral ischemiareperfusion injury in rats.

Key words: Hypoxia-ischemia, brain; Tumor necrosis factor;Interleukin-1; Inflammation; Rats, Sprague-Dawley